Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,246,914	T→C	intergenic (‑145/+149)	cirA ← / ← lysP	colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,246,914	0	T	C	100.0%	191.8 / NA	53	intergenic (‑145/+149)	cirA/lysP	colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

TCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGA  >  NC_000913/2246770‑2247059                                                                                                                                                 |                                                                                                                                                  tCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCt                                                                                                                                             >  2:41582/1‑151 (MQ=255)   cATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCttt                                                                                                                                           >  2:313249/1‑151 (MQ=255)          ccATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCtttttt                                                                                                                                        <  2:698001/147‑1 (MQ=255)           cATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTg                                                                                                                                   >  2:228372/1‑151 (MQ=255)           cATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTg                                                                                                                                   >  2:171491/1‑151 (MQ=255)                   aaCTACGAAAATAAAATGAGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTaa                                                                                                                           <  1:258135/151‑1 (MQ=255)                         gAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATaa                                                                                                                     <  2:536233/151‑1 (MQ=255)                            aataaaatGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGc                                                                                                                  >  2:598749/1‑151 (MQ=255)                             ataaaatGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGc                                                                                                                  >  1:618720/1‑150 (MQ=255)                             ataaaatGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGc                                                                                                                  >  1:61476/1‑150 (MQ=255)                               aaaatGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCaa                                                                                                                <  2:744596/150‑1 (MQ=255)                                aaatGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAAc                                                                                                              >  1:314360/1‑151 (MQ=255)                                      gTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAAc                                                                                                        <  1:239804/151‑1 (MQ=255)                                         aTCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAAt                                                                                                     <  1:37120/151‑1 (MQ=255)                                         aTCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAAt                                                                                                     >  2:72511/1‑151 (MQ=255)                                           cGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAAtt                                                                                                    <  2:618777/150‑1 (MQ=255)                                                acaTCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAAt                                                                                              >  1:395351/1‑151 (MQ=255)                                                acaTCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAAt                                                                                              <  2:521824/151‑1 (MQ=255)                                                acaTCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAAt                                                                                              >  2:252450/1‑151 (MQ=255)                                                     ttcttcACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCaa                                                                                          >  2:439785/1‑150 (MQ=255)                                                     ttcttcACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCaa                                                                                          >  2:128556/1‑150 (MQ=255)                                                          cACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTa                                                                                    <  2:128504/151‑1 (MQ=255)                                                               ccccTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGTTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAAtt                                                                                <  2:406611/150‑1 (MQ=255)                                                                cccTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAAtttt                                                                              <  1:647765/151‑1 (MQ=255)                                                                 ccTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTta                                                                             <  1:72480/151‑1 (MQ=255)                                                                        tACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCACAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGc                                                                      >  2:476574/1‑151 (MQ=255)                                                                           ggCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATg                                                                   <  1:344465/151‑1 (MQ=255)                                                                               gcaTCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGAttt                                                               <  2:114134/151‑1 (MQ=255)                                                                                 aTCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGt                                                             >  1:68875/1‑151 (MQ=255)                                                                                     cgGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAgg                                                         >  2:716484/1‑151 (MQ=255)                                                                                      ggTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAggt                                                        <  1:43467/151‑2 (MQ=255)                                                                                      ggTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAggg                                                        <  1:708094/151‑1 (MQ=255)                                                                                       gTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAggg                                                        >  1:542855/1‑150 (MQ=255)                                                                                         ggACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGaaa                                                     <  2:106501/151‑1 (MQ=255)                                                                                         ggACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGaa                                                      >  1:557168/1‑150 (MQ=255)                                                                                                  ttttATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTg                                             <  2:419657/150‑1 (MQ=255)                                                                                                     tATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGaaa                                          >  1:26806/1‑150 (MQ=255)                                                                                                     tATGCAGGTGATCATACCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCGTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCTATCCAAATGTTAAATTTTTTATCCGTATGATTTGTCAGGGAAATGGAATTGaa                                           >  1:345552/1‑149 (MQ=255)                                                                                                        gCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGc                                      >  1:9079/1‑151 (MQ=255)                                                                                                          aGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGcc                                     <  1:710937/150‑1 (MQ=255)                                                                                                              gATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCtctc                                <  2:322500/151‑1 (MQ=255)                                                                                                               atcatcCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCtctc                                <  2:680240/150‑1 (MQ=255)                                                                                                                tcatcCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCgg                              >  1:194094/1‑151 (MQ=255)                                                                                                                 catcCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGt                             >  2:763327/1‑151 (MQ=255)                                                                                                                  atcCCAGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGtt                            <  2:361348/151‑1 (MQ=255)                                                                                                                       aGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGagag                       <  1:228339/151‑1 (MQ=255)                                                                                                                       aGAAAGCCGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGagag                       <  1:171458/151‑1 (MQ=255)                                                                                                                             ccGGAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCtt                  >  2:676695/1‑150 (MQ=255)                                                                                                                                gAACAGCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCa              <  1:33879/151‑1 (MQ=255)                                                                                                                                     gCCTCTGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaagga          >  1:15423/1‑150 (MQ=255)                                                                                                                                         ctGATTTGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaaggaagg       <  2:2953/149‑1 (MQ=255)                                                                                                                                         cggATTTGCCAGCCGTTGTTGAGTGCGGCGTAACGATAATGCAAACGGTAACAATTCTCAATCCAAATGTTATATTTTATATCCGCCTGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCACGGAAggg      >  1:291018/3‑150 (MQ=255)                                                                                                                                             tttGCCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGggagga  >  2:403898/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGA  >  NC_000913/2246770‑2247059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑