Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,116	G→A	D93N (GAT→AAT)	nfo →	endonuclease IV with intrinsic 3'‑5' exonuclease activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,116	0	G	A	100.0%	208.2 / NA	55	D93N (GAT→AAT)	nfo	endonuclease IV with intrinsic 3'‑5' exonuclease activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

CACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACA  >  NC_000913/2250971‑2251262                                                                                                                                                  |                                                                                                                                                   cACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGa                                                                                                                                                <  2:725229/150‑1 (MQ=255)  acgacgCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGaa                                                                                                                                               >  1:107162/1‑150 (MQ=255)   cgacgCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGAGAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTAGAAAAATAGCGCGATGCCTTTATAAATGaaa                                                                                                                                              >  2:186852/1‑150 (MQ=255)        cAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGc                                                                                                                                        >  1:47720/1‑151 (MQ=255)          aaCCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGt                                                                                                                                      <  2:38/151‑1 (MQ=255)            ccATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTg                                                                                                                                    <  2:535427/151‑1 (MQ=255)               tCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCg                                                                                                                                  >  1:185919/1‑150 (MQ=255)                  aTGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACa                                                                                                                              <  1:750523/151‑1 (MQ=255)                    gAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGc                                                                                                                            <  1:317703/151‑1 (MQ=255)                     aaTTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCt                                                                                                                           >  1:303133/1‑151 (MQ=255)                     aaTTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCt                                                                                                                           >  2:71600/1‑151 (MQ=255)                       ttCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTgg                                                                                                                         >  1:224436/1‑151 (MQ=255)                         cAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTggg                                                                                                                        <  1:242183/150‑1 (MQ=255)                          aaaGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGc                                                                                                                      >  1:57823/1‑151 (MQ=255)                            aGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGctt                                                                                                                    <  1:605709/151‑1 (MQ=255)                               cgccTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGctttct                                                                                                                 <  2:49316/151‑1 (MQ=255)                                   tgtgAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGc                                                                                                             >  2:208559/1‑151 (MQ=255)                                    gtgAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCt                                                                                                            >  2:181342/1‑151 (MQ=255)                                      gAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTc                                                                                                           <  1:238611/150‑1 (MQ=255)                                      gAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCa                                                                                                          <  1:370055/151‑1 (MQ=255)                                          aaTATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACtt                                                                                                      <  2:352694/151‑1 (MQ=255)                                             atCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTcc                                                                                                    >  1:67062/1‑150 (MQ=255)                                             atCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCa                                                                                                   <  1:411915/151‑1 (MQ=255)                                             atCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCa                                                                                                   <  2:712210/151‑1 (MQ=255)                                                   acacATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTg                                                                                              <  1:676168/150‑1 (MQ=255)                                                      caTCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGc                                                                                            >  2:195312/1‑149 (MQ=255)                                                      caTCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAg                                                                                          <  1:512548/151‑1 (MQ=255)                                                           gcgcAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATc                                                                                     <  1:61610/151‑1 (MQ=255)                                                            cgcAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCt                                                                                    >  2:127400/1‑151 (MQ=255)                                                             gcAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTg                                                                                   >  2:234635/1‑151 (MQ=255)                                                                         ccACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTc                                                                        >  1:227567/1‑150 (MQ=255)                                                                              aCAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCaga                                                                     >  2:541380/1‑148 (MQ=255)                                                                              aCAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCaga                                                                     >  2:314961/1‑148 (MQ=255)                                                                               cAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAagag                                                                 <  1:406613/151‑1 (MQ=255)                                                                                aGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGAGTTGCGAACAGCTCGGGCTTTCTTTGCTCTACTTCCACACTGGCAGCCATCTGATGCAGATTTCagaaga                                                                  >  2:457470/1‑149 (MQ=255)                                                                                           tAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTgc                                                      <  2:391775/150‑1 (MQ=255)                                                                                            aaCCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTgcgc                                                    >  1:187882/1‑151 (MQ=255)                                                                                              ccTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGcgcg                                                   >  2:280428/1‑150 (MQ=255)                                                                                                         cGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATc                                       <  1:183671/151‑1 (MQ=255)                                                                                                             cACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAAAAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATc                                   <  2:344671/151‑1 (MQ=255)                                                                                                              aCTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCa                                  <  2:523550/151‑1 (MQ=255)                                                                                                                           aaaaaTAGCGCGATGCCTTTATAAATGAAATGCAGAGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCTACCCTGGCAGCAATCTGCTGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCGATCAACATAGCGATg                       >  1:380219/1‑149 (MQ=255)                                                                                                                            aaaaTCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGAt                    <  1:609542/151‑1 (MQ=255)                                                                                                                            aaaaTCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGAt                    <  1:24214/151‑1 (MQ=255)                                                                                                                                 cgcgcgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAAc               <  2:47751/151‑1 (MQ=255)                                                                                                                                   cgcgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTc             <  2:140983/151‑1 (MQ=255)                                                                                                                                   cgcgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTc             <  2:485505/151‑1 (MQ=255)                                                                                                                                      gATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAg          <  1:206893/151‑1 (MQ=255)                                                                                                                                       aTGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAgg         >  2:756929/1‑151 (MQ=255)                                                                                                                                          ccTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGgtgt      <  1:111717/151‑1 (MQ=255)                                                                                                                                             ttATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGAc   >  1:654260/1‑151 (MQ=255)                                                                                                                                             ttATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGAc   >  1:653599/1‑151 (MQ=255)                                                                                                                                               ataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGAc   >  2:223716/1‑149 (MQ=255)                                                                                                                                               ataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACa  <  2:187915/150‑1 (MQ=255)                                                                                                                                               ataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACa  <  1:690737/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACA  >  NC_000913/2250971‑2251262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑