Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,265,230 A→G G22G (GGT→GGC)  yeiW ← UPF0153 cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,265,2300AG97.4% 110.1 / ‑6.7 40G22G (GGT→GGC) yeiWUPF0153 cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (16/22);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

CTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGA  >  NC_000913/2265086‑2265372
                                                                                                                                                |                                                                                                                                              
ctgctgACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCggg                                                                                                                                           >  2:38295/1‑150 (MQ=255)
  gctgACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCggg                                                                                                                                           >  2:418407/1‑148 (MQ=255)
      aCGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAggg                                                                                                                                    <  2:44212/151‑1 (MQ=255)
           aGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCATCTGGCATGCCGGGGATAGGGCTgg                                                                                                                                >  2:197071/1‑150 (MQ=255)
                     caTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCCTCTGGCATGCCGGGGATAGGGGTGGGAATTGAAggg                                                                                                                     >  1:341116/1‑151 (MQ=255)
                       tttCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCCTGCCGTCTTGCTTACCGGGAAATAGGCTGGGAATTGAAggg                                                                                                                     >  1:361945/1‑149 (MQ=255)
                                 ggCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAacac                                                                                                         <  2:201684/151‑1 (MQ=255)
                                     tGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCCTCTGGCATGCCGGGGATAGGGGTGGGAATTGAAGGGGCAGTGCAACACGccc                                                                                                     >  1:620859/1‑151 (MQ=255)
                                     tGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATTTTGCATGCCGGGGATAGGGGTGGGAATTGAAGGGGCAGTGCAACACGccc                                                                                                     >  1:337965/1‑151 (MQ=255)
                                        aaGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGc                                                                                                  <  2:344354/151‑1 (MQ=255)
                                         aGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGcccc                                                                                                    >  2:225135/1‑148 (MQ=255)
                                         aGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCa                                                                                                 <  2:1812/151‑1 (MQ=255)
                                              gCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAAccc                                                                                             <  2:543743/150‑1 (MQ=255)
                                              gCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCg                                                                                            <  2:473214/151‑1 (MQ=255)
                                                  aGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACg                                                                                        <  2:699866/151‑1 (MQ=255)
                                                     cTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCATCTGGCATGCCGGGGATAGGGGTGGGAATTTGAGGGGGAGTGCAACACGCCCCCCAACCCGGGCgg                                                                                       >  1:427663/1‑149 (MQ=255)
                                                       ttCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCa                                                                                     <  1:526090/149‑1 (MQ=255)
                                                               cAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCCTGCCATCTTGCCTGCCCGGGATAGGGCCGGGAATTTAAGGGGCCGTGCAACACGCCCCGCCAACCGGACCGCATTcc                                                                                 >  2:15189/1‑145 (MQ=255)
                                                                     cGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTTGCATTCCGGGAATAGGGCTGGGAATTTAAGGGGCAGGGCAACACGCCCCGCCACCCGGGCGGCCTTCCATAAgttgtt                                                                       >  1:203547/1‑149 (MQ=255)
                                                                        ggTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCtt                                                                  <  1:457339/151‑1 (MQ=255)
                                                                        ggTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCtt                                                                  <  1:300120/151‑1 (MQ=255)
                                                                        ggTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCtt                                                                  <  2:306002/151‑1 (MQ=255)
                                                                           gAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCtt                                                                  >  2:471048/1‑148 (MQ=255)
                                                                               aTTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCCTCTGGCATGCCGGGGATAGGGCTGGGAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAgggt                                                            >  1:105849/1‑148 (MQ=255)
                                                                                        cGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGGAATTGAAGGGGCAGTGCAACACGCCCCGCAAACCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCgg                                                   >  2:430929/1‑150 (MQ=255)
                                                                                         gTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGAt                                                 <  2:729100/151‑1 (MQ=255)
                                                                                           tGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGAtt                                                <  1:516758/150‑1 (MQ=255)
                                                                                                 tcatcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGGCGGGAATAGGGCTGGGAATTGAAGGGGCAGGGCAACACGCCCCGCCACCCGGACGGCATTCCATACGGTGTTCTCTTAAGGGTAACCACCGGGTtga                                              >  2:9741/1‑146 (MQ=255)
                                                                                                   atcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAgcgc                                       <  1:272034/151‑1 (MQ=255)
                                                                                                    tcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGGTAACCACCGGATTGATGAgcgc                                       >  2:475847/1‑150 (MQ=255)
                                                                                                           gAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTa                               <  1:330026/151‑1 (MQ=255)
                                                                                                           gAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTa                               <  2:509724/151‑1 (MQ=255)
                                                                                                                           ggCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCAc                <  2:284202/150‑1 (MQ=255)
                                                                                                                             cGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAg             <  1:267760/151‑1 (MQ=255)
                                                                                                                              gggCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGc            >  2:289090/1‑151 (MQ=255)
                                                                                                                               ggCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCa           <  2:104722/151‑1 (MQ=255)
                                                                                                                                gCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCaa          <  1:115960/151‑1 (MQ=255)
                                                                                                                                    gCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGt      <  1:269902/151‑1 (MQ=255)
                                                                                                                                    gCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGt      <  1:225102/151‑1 (MQ=255)
                                                                                                                                         cTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGa  >  1:569169/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGA  >  NC_000913/2265086‑2265372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑