Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,304,235	A→G	K111E (AAG→GAG)	eco →	ecotin, a serine protease inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,304,235	0	A	G	100.0%	153.5 / NA	44	K111E (AAG→GAG)	eco	ecotin, a serine protease inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/21);  total (23/21)

TCGGTCAGACGCTGGAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCAAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACG  >  NC_000913/2304089‑2304379                                                                                                                                                   |                                                                                                                                                 tCGGTCAGACGCTGGAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCgaga                                                                                                                                               <  2:705419/150‑1 (MQ=255)  cGGTCAGACGCTGGAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCAGTTTCAACGATGATGGCCTGCCCGGATGGCgagaaa                                                                                                                                             <  2:213113/151‑1 (MQ=255)             tGGAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAAtt                                                                                                                                  >  2:454103/1‑151 (MQ=255)              ggAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAAtt                                                                                                                                  <  2:155439/150‑1 (MQ=255)                 aGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGGAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGGTTCAACGATGATGGCCTGCCCGGATGGCGGGAAAGAGAAGAAAttt                                                                                                                                 >  1:331589/1‑148 (MQ=255)                 aGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTc                                                                                                                              <  2:40930/151‑1 (MQ=255)                          cAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTAt                                                                                                                     <  1:135805/151‑1 (MQ=255)                           aaTTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATc                                                                                                                    <  2:331622/151‑1 (MQ=255)                           aaTTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAAAGCTGGAAGGCTGGGGGTTTGGTTATTATTTCTTTGGTAAAGTCAGTTCCCCCGGGTCCACCATTATGGCCTGCCCGGGTGGCGAGGAAGAGCAGAAATTTGTCACcgcgctt                                                                                                                     >  1:753497/1‑147 (MQ=255)                             tttGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTg                                                                                                                  >  2:706450/1‑151 (MQ=255)                              ttGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTTTGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGGTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTgg                                                                                                                 >  2:516934/1‑151 (MQ=255)                                gCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGc                                                                                                               <  2:524967/151‑1 (MQ=255)                                      tctcGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGGGGGAAAGGAAAGAAATTGTTCACCGGGTAttcgg                                                                                                                 >  2:528616/1‑139 (MQ=255)                                         cggcggGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCt                                                                                                         <  1:609849/148‑1 (MQ=255)                                            cggGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAAtg                                                                                                   >  2:719637/1‑151 (MQ=255)                                             gggAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAAtgc                                                                                                  >  1:326370/1‑151 (MQ=255)                                                       aaaacaaaacGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTacaa                                                                                        <  1:456460/151‑1 (MQ=255)                                                                  cTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGc                                                                             >  2:124784/1‑151 (MQ=255)                                                                         gCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCgt                                                                      >  2:101980/1‑151 (MQ=255)                                                                          cTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCgtg                                                                     >  2:87029/1‑151 (MQ=255)                                                                          cTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCgtg                                                                     >  1:711505/1‑151 (MQ=255)                                                                            ggggCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCgtggt                                                                   <  2:183466/151‑1 (MQ=255)                                                                            ggggCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCgtg                                                                     <  2:332728/149‑1 (MQ=255)                                                                             gggCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCgtggtg                                                                  <  1:191313/151‑1 (MQ=255)                                                                               gCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGgtgt                                                                 >  2:115248/1‑150 (MQ=255)                                                                                        attatGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGcc                                                          >  1:60930/1‑148 (MQ=255)                                                                                           atGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACa                                                     >  1:236829/1‑150 (MQ=255)                                                                                                tttGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGt                                                 >  2:79302/1‑149 (MQ=255)                                                                                                tttGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGt                                                 >  1:671098/1‑149 (MQ=255)                                                                                                 ttGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTa                                                <  1:398638/149‑1 (MQ=255)                                                                                                 ttGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAg                                               <  2:741007/150‑1 (MQ=255)                                                                                                   gATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGAtg                                            <  1:352492/151‑1 (MQ=255)                                                                                                   gATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGACCGTGGTGTATACGCCAGACAATGTAGAt                                             <  2:623909/150‑1 (MQ=255)                                                                                                      aaaGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGAtgtg                                          >  2:516432/1‑150 (MQ=255)                                                                                                            aGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTAcc                                   >  2:299234/1‑151 (MQ=255)                                                                                                            aGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTAcc                                   >  1:522361/1‑151 (MQ=255)                                                                                                                   cGGTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTGGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCCATCGTGGTGTATCCGCCAGACAATGTAGATGTGAAGTACCGCGTCTg                            <  1:9359/151‑1 (MQ=255)                                                                                                                    ggTTTCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTgg                           <  2:389550/151‑1 (MQ=255)                                                                                                                       ttCAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGa                          >  2:246538/1‑149 (MQ=255)                                                                                                                         cAACGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGGATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAgg                       <  1:496819/150‑1 (MQ=255)                                                                                                                            cGATGATGGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCgg                    >  1:728554/1‑150 (MQ=255)                                                                                                                              atgatgGCCTGCCCGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAag                 <  1:269499/151‑1 (MQ=255)                                                                                                                                         cccGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGAc      >  2:47995/1‑151 (MQ=255)                                                                                                                                          ccGGATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACa     <  1:86998/151‑1 (MQ=255)                                                                                                                                           cGGATGGCGAGAAAGAGAAGAAATTCGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACaa    <  1:240000/151‑1 (MQ=255)                                                                                                                                             gATGGCGAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAAcg  <  2:236862/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TCGGTCAGACGCTGGAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAAAACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTGCCCGGATGGCAAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACG  >  NC_000913/2304089‑2304379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑