Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,310,335 T→C D24G (GAC→GGC)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,310,3350TC97.8% 147.6 / ‑3.8 45D24G (GAC→GGC) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (20/24);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

ACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTA  >  NC_000913/2310190‑2310481
                                                                                                                                                 |                                                                                                                                                  
aCAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCa                                                                                                                                               <  2:597366/151‑1 (MQ=255)
 cAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCAt                                                                                                                                              <  2:98668/151‑1 (MQ=255)
  aaCGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCAtt                                                                                                                                             <  2:399898/151‑1 (MQ=255)
  aaCGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCAtt                                                                                                                                             >  2:439273/1‑151 (MQ=255)
        tGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTc                                                                                                                                       >  1:267290/1‑151 (MQ=255)
         gCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTcg                                                                                                                                      <  1:310417/151‑1 (MQ=255)
         gCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTc                                                                                                                                       <  2:98293/150‑1 (MQ=255)
         gCAGGAGCGAACGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCCCGGCGAACACGTATTCGCCGCCGGCATTCGGGTcg                                                                                                                                      >  1:169948/1‑151 (MQ=255)
           aGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTcgcg                                                                                                                                    >  1:196284/1‑151 (MQ=255)
           aGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTcgcg                                                                                                                                    <  2:238867/151‑1 (MQ=255)
             gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCggg                                                                                                                                  <  2:585126/151‑1 (MQ=255)
               gCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCt                                                                                                                                >  2:466257/1‑151 (MQ=255)
                cGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCt                                                                                                                                >  2:53242/1‑150 (MQ=255)
                             cgAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCa                                                                                                                  <  1:361435/151‑1 (MQ=255)
                              gAGTGCCTCGCTGGCATTAGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAGGACGGACGGCAAAAGATGCCTGTGGTACGAACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGcc                                                                                                                   >  1:384151/1‑149 (MQ=255)
                                      cGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGa                                                                                                           <  1:723959/149‑1 (MQ=255)
                                      cGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATc                                                                                                         <  2:399636/151‑1 (MQ=255)
                                        cTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGt                                                                                                       >  1:37073/1‑151 (MQ=255)
                                           gCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGt                                                                                                       <  1:546982/148‑1 (MQ=255)
                                           gCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAg                                                                                                    <  2:267323/151‑1 (MQ=255)
                                             aTTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGtt                                                                                                  >  2:134999/1‑151 (MQ=255)
                                               ttGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTa                                                                                                 <  2:739822/150‑1 (MQ=255)
                                                tGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTa                                                                                                 >  1:548303/1‑149 (MQ=255)
                                                     aGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCAtgt                                                                                          <  2:415631/151‑1 (MQ=255)
                                                       aaGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTgg                                                                                        >  1:127366/1‑151 (MQ=255)
                                                                   tCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATa                                                                             >  2:710734/1‑150 (MQ=255)
                                                                    cccGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAAt                                                                           <  1:311623/151‑1 (MQ=255)
                                                                        cAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGc                                                                       >  2:177214/1‑151 (MQ=255)
                                                                        cAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGc                                                                       <  2:186620/151‑1 (MQ=255)
                                                                              aTGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTg                                                                 >  2:711760/1‑151 (MQ=255)
                                                                              aTGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTg                                                                 >  2:661097/1‑151 (MQ=255)
                                                                                 tCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGtt                                                              >  1:30743/1‑151 (MQ=255)
                                                                                    ggCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTa                                                             <  2:352008/149‑1 (MQ=255)
                                                                                        cgGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGAt                                                        >  2:455454/1‑150 (MQ=255)
                                                                                                 ggacggCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAgg                                              <  2:278068/151‑1 (MQ=255)
                                                                                                   acggCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCt                                            >  1:239228/1‑151 (MQ=255)
                                                                                                   acggCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCt                                            >  1:201301/1‑151 (MQ=255)
                                                                                                      gCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGcc                                         <  1:76261/151‑1 (MQ=255)
                                                                                                                   gtgGTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCa                             <  1:302200/150‑1 (MQ=255)
                                                                                                                     ggTACGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGc                          <  1:94181/151‑1 (MQ=255)
                                                                                                                        aCGCACGGCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCaa                        <  2:255351/150‑1 (MQ=255)
                                                                                                                              ggCGAAAACGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACcaa                 <  1:140404/151‑1 (MQ=255)
                                                                                                                                  aaaaCGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACcaac                <  1:144946/148‑1 (MQ=255)
                                                                                                                                  aaaaCGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATaa                                                                            >  1:702768/1‑88 (MQ=255)
                                                                                                                                  aaaaCGAATTCGCCGCCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATaa                                                                            <  2:702842/88‑1 (MQ=255)
                                                                                                                                            cgccgcCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCttt   <  2:635352/151‑1 (MQ=255)
                                                                                                                                              ccgccgGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTa  >  2:173139/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTA  >  NC_000913/2310190‑2310481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑