Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,634	T→C	Q511R (CAG→CGG)	nuoG ←	NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,634	0	T	C	100.0%	180.0 / NA	51	Q511R (CAG→CGG)	nuoG	NADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/22);  total (29/22)

GTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGG  >  NC_000913/2398488‑2398780                                                                                                                                                   |                                                                                                                                                   gTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAAt                                                                                                                                                <  1:170486/151‑1 (MQ=255)  tacGGTTTCCAGTTCGGATAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCTTTCCGTTGACGGAACGTTTATTCATGGTTATACCGACGTCAGCCCCGCGACCTTTCAGGCCTTCCGCGACTTTAGCCGCCCCCCGAAt                                                                                                                                                >  1:451142/3‑150 (MQ=255)    cGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCAc                                                                                                                                             <  1:259669/151‑1 (MQ=255)     ggTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCAcc                                                                                                                                            <  2:730505/151‑1 (MQ=255)                 ggTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCg                                                                                                                                >  2:600759/1‑151 (MQ=255)                   ttAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGc                                                                                                                              >  1:492291/1‑151 (MQ=255)                     aaCGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCg                                                                                                                             <  1:575840/150‑1 (MQ=255)                       cGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTc                                                                                                                          >  2:68991/1‑151 (MQ=255)                        gCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCg                                                                                                                         >  2:742273/1‑151 (MQ=255)                              tcAAGCGAACCGCCACCCATAATGACCAGCCCCATGCTGTTGACGGAACGGGCAATCACGGTGATACCGACGTCAGCGCCGCGACCTTTCAGCGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCgg                                                                                                                   >  1:556156/1‑151 (MQ=255)                                 aGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCgg                                                                                                                   >  2:103882/1‑148 (MQ=255)                                 aGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCgg                                                                                                                   >  2:342509/1‑148 (MQ=255)                                       ccGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATc                                                                                                           >  2:694471/1‑150 (MQ=255)                                               cATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTttc                                                                                                  <  1:75687/151‑1 (MQ=255)                                                     gCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGc                                                                                             <  1:267887/150‑1 (MQ=255)                                                     gCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCa                                                                                            <  1:463431/151‑1 (MQ=255)                                                       ccAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCGGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATATTCAACGGTTTCTTCGCAc                                                                                           >  1:548587/1‑150 (MQ=255)                                                           ccccATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGcc                                                                                      >  2:762687/1‑151 (MQ=255)                                                               aTGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTg                                                                                  <  1:305745/151‑1 (MQ=255)                                                                       gACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACg                                                                          <  1:342475/151‑1 (MQ=255)                                                                          ggAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATg                                                                       <  2:327280/151‑1 (MQ=255)                                                                            aaCGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGAc                                                                     <  2:343310/151‑1 (MQ=255)                                                                            aaCGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGAc                                                                     >  1:254965/1‑151 (MQ=255)                                                                            aaCGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGAc                                                                     >  1:214062/1‑151 (MQ=255)                                                                               gggCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTc                                                                  >  2:628879/1‑151 (MQ=255)                                                                               gggCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTc                                                                  <  2:213160/151‑1 (MQ=255)                                                                                 gCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCg                                                                 >  2:499312/1‑150 (MQ=255)                                                                                       aTGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttgc                                                          <  1:646571/151‑1 (MQ=255)                                                                                           tGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttgctttg                                                      >  1:136469/1‑151 (MQ=255)                                                                                               aCCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAg                                                   <  1:189069/150‑1 (MQ=255)                                                                                                ccGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCt                                                 <  2:762293/151‑1 (MQ=255)                                                                                                 cGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTc                                                >  1:686095/1‑151 (MQ=255)                                                                                                   aCGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTc                                                >  1:384511/1‑149 (MQ=255)                                                                                                       cAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGtt                                           <  2:603472/150‑1 (MQ=255)                                                                                                       cAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTc                                          >  1:391158/1‑151 (MQ=255)                                                                                                         gcgcCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCg                                         <  1:310480/150‑1 (MQ=255)                                                                                                                gACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTc                                 >  1:733549/1‑151 (MQ=255)                                                                                                                gACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTc                                 <  1:548641/151‑1 (MQ=255)                                                                                                                 aCCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCa                                <  1:742199/151‑1 (MQ=255)                                                                                                                    tttCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAAcc                             >  1:109105/1‑151 (MQ=255)                                                                                                                       cAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCt                          >  1:398585/1‑151 (MQ=255)                                                                                                                         gggCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTgg                        >  1:647582/1‑151 (MQ=255)                                                                                                                                 gcgACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGt                <  2:201984/151‑1 (MQ=255)                                                                                                                                   gACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTa              <  2:589286/151‑1 (MQ=255)                                                                                                                                      gTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATAcc                                    <  2:776814/126‑1 (MQ=255)                                                                                                                                      gTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATAcc                                    >  1:776738/1‑126 (MQ=255)                                                                                                                                       ttAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATcc           >  1:432956/1‑150 (MQ=255)                                                                                                                                        tAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATcc           >  1:642808/1‑149 (MQ=255)                                                                                                                                        tAGCCCCCGCCCGAATCACCTCTAAGCTACCCGCGTTCGTCCCGGAGACAACCAACGGCTTCTTCGCACCTCCCAGCCCCTGCACGATCACGCCCCTTTTCCCTTGCAGCCCAGGCTCGCTACCGTCAACCGCTGGCGCAGCGCTATcc           >  1:22812/1‑149 (MQ=255)                                                                                                                                            cgccgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgcgc      >  2:595807/1‑150 (MQ=255)                                                                                                                                               cgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATgg  >  1:187888/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGG  >  NC_000913/2398488‑2398780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑