Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,437,826	A→G	M9T (ATG→ACG)	pdxB ←	erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,437,826	0	A	G	100.0%	192.4 / NA	52	M9T (ATG→ACG)	pdxB	erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/33);  total (19/33)

GGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTATTTCCGGCC  >  NC_000913/2437679‑2437971                                                                                                                                                    |                                                                                                                                                  ggTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCgtat                                                                                                                                                <  2:222116/151‑1 (MQ=255)     ttCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTAtttt                                                                                                                                             <  1:558109/150‑1 (MQ=255)     ttCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTAtttt                                                                                                                                             <  1:378597/150‑1 (MQ=255)      tCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTtc                                                                                                                                            >  1:34134/1‑150 (MQ=255)       ccTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTtca                                                                                                                                           <  2:10959/150‑1 (MQ=255)           ccAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATcaac                                                                                                                                      <  1:556542/151‑1 (MQ=255)                  aGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGAtt                                                                                                                               <  2:474994/151‑1 (MQ=255)                   gATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGAttt                                                                                                                              <  2:530288/151‑1 (MQ=255)                      tcattcaCTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTc                                                                                                                            >  1:227803/1‑150 (MQ=255)                          tcaCTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGt                                                                                                                        <  1:417614/150‑1 (MQ=255)                           caCTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATcaacaa                                                                                                                                    >  1:604256/1‑137 (MQ=255)                           caCTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATcaacaa                                                                                                                                    <  2:604313/137‑1 (MQ=255)                             cTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTtg                                                                                                                     <  1:765632/150‑1 (MQ=255)                                tCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCAc                                                                                                                          >  2:155910/1‑142 (MQ=255)                                tCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCAc                                                                                                                          <  1:155877/142‑1 (MQ=255)                                tCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTgtgt                                                                                                                  <  1:587859/150‑1 (MQ=255)                                 cGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTa                                                                                                                <  1:552976/151‑1 (MQ=255)                                  gTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTAc                                                                                                               <  2:632001/151‑1 (MQ=255)                                    gACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCt                                                                                                             >  2:364716/1‑151 (MQ=255)                                      ccGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGt                                                                                                           <  1:128581/151‑1 (MQ=255)                                           cGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATgag                                                                                                      <  1:756156/151‑1 (MQ=255)                                             cACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATgaga                                                                                                     <  2:463655/150‑1 (MQ=255)                                             cACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGAc                                                                                                    <  2:642191/151‑1 (MQ=255)                                                 aTCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACgaga                                                                                                <  1:684125/151‑1 (MQ=255)                                                   cAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGagag                                                                                               <  2:50620/150‑1 (MQ=255)                                                            cgcgTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGAc                                                                                     >  2:529581/1‑151 (MQ=255)                                                                 cTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTATGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAgtg                                                                                >  2:632784/1‑151 (MQ=255)                                                                                   ggATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGcc                                                              <  1:117544/151‑1 (MQ=255)                                                                                     aTTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGcc                                                              >  2:390396/1‑149 (MQ=255)                                                                                       tGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGc                                                          >  1:659205/1‑151 (MQ=255)                                                                                        ggACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGc                                                          <  1:668186/150‑1 (MQ=255)                                                                                        ggACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGc                                                          >  2:330763/1‑150 (MQ=255)                                                                                              ccGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTg                                                   <  1:728985/151‑1 (MQ=255)                                                                                                gggAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCg                                                 <  1:501061/151‑1 (MQ=255)                                                                                                   aaCCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAg                                              <  2:431011/151‑1 (MQ=255)                                                                                                         ggTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGGGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCa                                        <  1:76740/151‑1 (MQ=255)                                                                                                          gTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAg                                       <  2:97331/151‑1 (MQ=255)                                                                                                            cacctcaccCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGt                                     >  1:538012/1‑151 (MQ=255)                                                                                                                 caccCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATaa                                 <  2:41002/150‑1 (MQ=255)                                                                                                                    ccAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGAt                             <  1:390347/151‑1 (MQ=255)                                                                                                                       aaCGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACg                          >  1:212517/1‑151 (MQ=255)                                                                                                                              aaaTAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGa                    <  2:489213/150‑1 (MQ=255)                                                                                                                              aaaTAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGa                    <  1:389360/150‑1 (MQ=255)                                                                                                                                aTAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATAc                 <  2:168889/151‑1 (MQ=255)                                                                                                                                 tAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATAc                 >  2:203214/1‑150 (MQ=255)                                                                                                                                  aaGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACaa               <  1:614865/151‑1 (MQ=255)                                                                                                                                   aGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAAc              >  2:651724/1‑151 (MQ=255)                                                                                                                                   aGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAAc              >  2:772457/1‑151 (MQ=255)                                                                                                                                         gggCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTAttt        <  2:501313/151‑1 (MQ=255)                                                                                                                                         gggCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTAttt        >  1:105171/1‑151 (MQ=255)                                                                                                                                          ggCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTATTTc       >  1:237655/1‑151 (MQ=255)                                                                                                                                               aaGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTATTTCCGGcc  >  2:204756/1‑151 (MQ=255)                                                                                                                                               aaGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGGATAAGGATACGTAATGATACAACCTATTTCCGGcc  >  2:229692/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTATTTCCGGCC  >  NC_000913/2437679‑2437971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑