Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,456,380	T→C	T145A (ACA→GCA)	sixA ←	phosphohistidine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,456,380	0	T	C	100.0%	186.0 / NA	52	T145A (ACA→GCA)	sixA	phosphohistidine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/24);  total (28/24)

TTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGT  >  NC_000913/2456238‑2456527                                                                                                                                               |                                                                                                                                                    ttCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGtt                                                                                                                                               <  1:604949/149‑1 (MQ=255)     aTAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCt                                                                                                                                         <  1:301956/151‑1 (MQ=255)       agtagtGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCttt                                                                                                                                       >  2:695253/1‑151 (MQ=255)        gtagtGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTtc                                                                                                                                      >  2:492589/1‑151 (MQ=255)        gtagtGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTtc                                                                                                                                      <  1:444442/151‑1 (MQ=255)          agtGCGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTtcat                                                                                                                                    >  1:248916/1‑151 (MQ=255)          agtGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTtcat                                                                                                                                    >  2:356815/1‑151 (MQ=255)           gtGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTtcatc                                                                                                                                   <  1:734408/151‑1 (MQ=255)               ggTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGc                                                                                                                               >  2:331251/1‑151 (MQ=255)                   gACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGtt                                                                                                                            >  1:451659/1‑150 (MQ=255)                            ccGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCa                                                                                                                   <  2:425706/150‑1 (MQ=255)                               cTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATg                                                                                                                >  1:53623/1‑150 (MQ=255)                                 gCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATgg                                                                                                               <  1:446698/149‑1 (MQ=255)                                 gCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATg                                                                                                                <  2:53654/148‑1 (MQ=255)                                 gCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGcc                                                                                                             <  2:732475/151‑1 (MQ=255)                                  ccTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCg                                                                                                            <  2:383751/151‑1 (MQ=255)                                     gatgaGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAg                                                                                                          <  1:149795/150‑1 (MQ=255)                                        gaGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAggtgg                                                                                                      <  2:570922/151‑1 (MQ=255)                                               tATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACa                                                                                                <  1:701344/150‑1 (MQ=255)                                                    ccAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAggcg                                                                                          <  2:630559/151‑1 (MQ=255)                                                     cAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAggcgg                                                                                         >  2:351017/1‑151 (MQ=255)                                                          aaTCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGttt                                                                                    >  1:744690/1‑151 (MQ=255)                                                              tACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGcc                                                                                >  2:612077/1‑151 (MQ=255)                                                               aCCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTc                                                                                   <  1:646369/147‑1 (MQ=255)                                                               aCCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGccc                                                                               >  2:367000/1‑151 (MQ=255)                                                                ccTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCg                                                                              >  1:270156/1‑151 (MQ=255)                                                                    aTTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACa                                                                          <  1:603829/151‑1 (MQ=255)                                                                              aTTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGc                                                                 >  1:459710/1‑150 (MQ=255)                                                                                        catcaGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATa                                                        <  1:193292/149‑1 (MQ=255)                                                                                            aGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGAc                                                  >  2:708836/1‑151 (MQ=255)                                                                                            aGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGAc                                                  <  1:659558/151‑1 (MQ=255)                                                                                             gATAGCTTTTGCCATCTTCAGATTACCCGGGACCATCTGCCAGTTGACTGCGCCGTTTCCGCTTTCATCAAGCGTCACGCTGGCAATGGCCGAGGAGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACt                                                 >  1:315683/1‑151 (MQ=255)                                                                                                   ttttGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGt                                           >  2:149612/1‑151 (MQ=255)                                                                                                      tGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTa                                          >  2:586379/1‑149 (MQ=255)                                                                                                       gCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAg                                        >  1:279913/1‑150 (MQ=255)                                                                                                       gCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGt                                       >  1:529115/1‑151 (MQ=255)                                                                                                        ccATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGTCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGt                                       >  2:207403/1‑150 (MQ=255)                                                                                                         cATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTg                                      >  1:263688/1‑150 (MQ=255)                                                                                                             ttCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgag                                    >  2:242794/1‑148 (MQ=255)                                                                                                              tCAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgag                                    >  1:366984/1‑147 (MQ=255)                                                                                                               cAGATTACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGAt                                <  2:165594/150‑1 (MQ=255)                                                                                                                    tACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCAcc                            >  2:537893/1‑149 (MQ=255)                                                                                                                    tACACGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCa                           <  2:165978/150‑1 (MQ=255)                                                                                                                     acacGGGCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGCGAGATCACCAGt                         >  1:586745/1‑151 (MQ=255)                                                                                                                         gggCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCg                     <  1:225424/151‑1 (MQ=255)                                                                                                                          ggCTCATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCg                     >  1:120022/1‑150 (MQ=255)                                                                                                                              cATCTGCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGc                 <  2:750075/150‑1 (MQ=255)                                                                                                                                   gCCAGTTGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACAcc           <  1:197107/151‑1 (MQ=255)                                                                                                                                        ttGAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCAt      >  1:369122/1‑151 (MQ=255)                                                                                                                                          gAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCAtt     <  1:24474/150‑1 (MQ=255)                                                                                                                                          gAATGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTg    >  1:451804/1‑151 (MQ=255)                                                                                                                                            aTGCGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGt  <  1:77343/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    TTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGT  >  NC_000913/2456238‑2456527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑