Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,459,668	T→C	T268A (ACG→GCG)	fadI ←	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,459,668	0	T	C	100.0%	221.4 / NA	64	T268A (ACG→GCG)	fadI	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/32);  total (32/32)

AACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCC  >  NC_000913/2459523‑2459812                                                                                                                                                  |                                                                                                                                                 aaCATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCg                                                                                                                                             >  2:681380/1‑151 (MQ=255) aaCATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCg                                                                                                                                             <  2:166137/151‑1 (MQ=255)    aTGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTc                                                                                                                                          >  2:385138/1‑151 (MQ=255)        ccTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCgtg                                                                                                                                      <  2:630035/151‑1 (MQ=255)                aCATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGAt                                                                                                                              >  1:148342/1‑151 (MQ=255)                  aTCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCGGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATc                                                                                                                             >  2:280709/1‑150 (MQ=255)                  aTCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCa                                                                                                                            <  2:249628/151‑1 (MQ=255)                    cAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCaa                                                                                                                           >  1:752501/1‑150 (MQ=255)                     aaTCGCAGCAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAAc                                                                                                                         >  2:549473/1‑151 (MQ=255)                         gCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCg                                                                                                                     >  2:150245/1‑151 (MQ=255)                           aGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCggg                                                                                                                   >  1:188320/1‑151 (MQ=255)                            gTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGgc                                                                                                                  <  1:158648/151‑1 (MQ=255)                               aaTGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCa                                                                                                               <  1:170800/151‑1 (MQ=255)                                  gCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCt                                                                                                            <  2:328205/151‑1 (MQ=255)                                    gTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCttt                                                                                                          >  2:500017/1‑151 (MQ=255)                                      aGCTGCGCAGATACCCCGGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGc                                                                                                        <  1:280676/151‑1 (MQ=255)                                      aGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGc                                                                                                        <  1:344771/151‑1 (MQ=255)                                       gCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCg                                                                                                       >  1:86412/1‑151 (MQ=255)                                             cAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCg                                                                                                 >  1:707852/1‑151 (MQ=255)                                                aTACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGc                                                                                               >  1:85364/1‑150 (MQ=255)                                                   ccccAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCaa                                                                                             <  2:467540/149‑1 (MQ=255)                                                     ccAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCg                                                                                          >  2:500874/1‑150 (MQ=255)                                                      cAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCg                                                                                          >  2:159305/1‑149 (MQ=255)                                                        gCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGa                                                                                      <  1:207642/151‑1 (MQ=255)                                                         cGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAgg                                                                                       <  2:416030/149‑1 (MQ=255)                                                          ggCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAAt                                                                                    <  1:353926/151‑1 (MQ=255)                                                          ggCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAAt                                                                                    <  2:646110/151‑1 (MQ=255)                                                          ggCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAAt                                                                                    <  1:389579/151‑1 (MQ=255)                                                            cACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAAtt                                                                                   >  1:202102/1‑150 (MQ=255)                                                            cACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTa                                                                                  <  1:668/151‑1 (MQ=255)                                                             aCCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTa                                                                                  <  1:606883/150‑1 (MQ=255)                                                               cAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTAcc                                                                                >  2:395014/1‑150 (MQ=255)                                                                 gCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACcgcg                                                                             >  1:307524/1‑151 (MQ=255)                                                                   ccTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGaa                                                                           <  2:311451/151‑1 (MQ=255)                                                                   ccTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGaa                                                                           <  1:95541/151‑1 (MQ=255)                                                                      aaTTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAatat                                                                        <  1:500822/151‑1 (MQ=255)                                                                        ttCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttg                                                                      >  2:78080/1‑151 (MQ=255)                                                                           tttCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttgttg                                                                   >  2:693856/1‑151 (MQ=255)                                                                            tccGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttgttg                                                                   <  1:34820/148‑1 (MQ=255)                                                                              cGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCt                                                                >  2:637523/1‑151 (MQ=255)                                                                              cGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCAGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCt                                                                >  2:687437/1‑151 (MQ=255)                                                                                 ccGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCg                                                             <  2:186681/151‑1 (MQ=255)                                                                                  cGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCg                                                             >  1:177055/1‑150 (MQ=255)                                                                                      aTTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACa                                                          >  2:48864/1‑149 (MQ=255)                                                                                           gTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGgtt                                                   <  1:118857/151‑1 (MQ=255)                                                                                              atcaGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGgttgtt                                                >  2:664867/1‑151 (MQ=255)                                                                                                   gATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATaa                                           <  1:34894/151‑1 (MQ=255)                                                                                                    aTCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAag                                          <  1:375787/151‑1 (MQ=255)                                                                                                    aTCACCGCTGCCGCGCCATCGGTCAGCAGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAag                                          <  1:687363/151‑1 (MQ=255)                                                                                                      cACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                                         <  1:717696/150‑1 (MQ=255)                                                                                                          gCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAggg                                     >  2:197359/1‑150 (MQ=255)                                                                                                          gCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAggg                                     >  1:158479/1‑150 (MQ=255)                                                                                                          gCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAggg                                     >  2:315128/1‑150 (MQ=255)                                                                                                          gCTGCCGCGCCACCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAaggag                                       <  1:16996/148‑1 (MQ=255)                                                                                                             gCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATa                                  >  2:142055/1‑150 (MQ=255)                                                                                                                  acgaTCGATCCGCGGCGTACTGTTTGCCGCGGCTAGCGTTCCGTGTTTGCGATCAATCGCCGCGCGCAGCTTTGCGTAAGCGGCAAGCGAGGAATTACCGCGAATATTGTTGTATTCGACAAGCGGTTGTTTATAAGGAGGAATAAATGCa                            <  1:728060/148‑1 (MQ=255)                                                                                                                         gTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGtcatca                     <  1:705482/151‑1 (MQ=255)                                                                                                                          tCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCAc                    >  1:384863/1‑151 (MQ=255)                                                                                                                            aGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACct                  >  1:521182/1‑151 (MQ=255)                                                                                                                                  gTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTcttctt            >  2:735864/1‑151 (MQ=255)                                                                                                                                    aCTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTg          <  2:775780/151‑1 (MQ=255)                                                                                                                                     cTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTg          >  1:511467/1‑150 (MQ=255)                                                                                                                                            ccgccgCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTcc  <  2:511519/151‑1 (MQ=255)                                                                                                                                            ccgccgCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTcc  <  2:696042/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 AACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCC  >  NC_000913/2459523‑2459812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑