Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,163	T→C	V106A (GTG→GCG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,163	0	T	C	100.0%	138.4 / NA	38	V106A (GTG→GCG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/22);  total (16/22)

CGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTG  >  NC_000913/2478026‑2478308                                                                                                                                          |                                                                                                                                                  cGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTg                                                                                                                                      >  1:442192/1‑151 (MQ=255)                   cGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCtg                                                                                                                    <  2:276038/151‑1 (MQ=255)                    gCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCtgt                                                                                                                   >  2:140790/1‑151 (MQ=255)                               cGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTg                                                                                                        >  2:566492/1‑151 (MQ=255)                                 gCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTggg                                                                                                      <  1:97010/151‑1 (MQ=255)                                 gCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTggg                                                                                                      >  1:486987/1‑151 (MQ=255)                                         aCGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGc                                                                                              <  1:197516/151‑1 (MQ=255)                                          cGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCt                                                                                             >  2:567748/1‑151 (MQ=255)                                          cGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCt                                                                                             >  1:458432/1‑151 (MQ=255)                                           gATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTa                                                                                            <  1:526272/151‑1 (MQ=255)                                                tGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGAt                                                                                       >  2:83436/1‑151 (MQ=255)                                                   gAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTcc                                                                                    <  2:539788/151‑1 (MQ=255)                                                       aaTGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTg                                                                                >  1:200460/1‑151 (MQ=255)                                                            tgGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCttt                                                                           >  1:10347/1‑151 (MQ=255)                                                                aGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTc                                                                        >  1:51140/1‑150 (MQ=255)                                                                aGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCa                                                                       <  2:312026/151‑1 (MQ=255)                                                                 gTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCaa                                                                      <  1:58384/151‑1 (MQ=255)                                                                 gTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCa                                                                       >  2:372482/1‑150 (MQ=255)                                                                         ggCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCaaa                                                              <  1:711286/151‑1 (MQ=255)                                                                           ccGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCaaaaa                                                            <  2:227359/151‑1 (MQ=255)                                                                                         ggTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCa                                               <  1:679608/150‑1 (MQ=255)                                                                                              gACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCatta                                            <  1:48949/148‑1 (MQ=255)                                                                                              gACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTAGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCAttatta                                         <  1:522148/151‑1 (MQ=255)                                                                                               acacTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTaa                                        <  2:68364/151‑1 (MQ=255)                                                                                                     cAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCtt                                   <  2:247088/150‑1 (MQ=255)                                                                                                       aCGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGcc                                >  2:615471/1‑151 (MQ=255)                                                                                                        cGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCa                               <  1:478567/151‑1 (MQ=255)                                                                                                           tGCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTc                            >  1:695853/1‑151 (MQ=255)                                                                                                            gCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCAtt                             <  2:513693/149‑1 (MQ=255)                                                                                                            gCCGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTcc                           <  2:237065/151‑1 (MQ=255)                                                                                                              cGCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGc                         <  2:680226/151‑1 (MQ=255)                                                                                                               gCTGGCTTTCAGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCt                        >  2:157976/1‑151 (MQ=255)                                                                                                                         aGTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGc               >  2:749080/1‑150 (MQ=255)                                                                                                                          gTTGATGTCATTATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCAt             <  1:692857/151‑1 (MQ=255)                                                                                                                                 tCATGATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATg        <  1:236072/149‑1 (MQ=255)                                                                                                                                    ttATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGt   >  2:404229/1‑151 (MQ=255)                                                                                                                                     tATGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGt   <  1:589875/150‑1 (MQ=255)                                                                                                                                      aTGGCGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTg  <  2:200492/150‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                  CGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTG  >  NC_000913/2478026‑2478308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑