Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,755	C→T	S193N (AGC→AAC)	ecpC ←	ECP production outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,755	0	C	T	100.0%	119.8 / NA	33	S193N (AGC→AAC)	ecpC	ECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/6);  total (27/6)

CCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTGAC  >  NC_000913/308608‑308900                                                                                                                                                    |                                                                                                                                                  ccGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTgg                                                                                                                                                >  2:693838/1‑151 (MQ=255)  cGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTgg                                                                                                                                                >  2:319263/1‑150 (MQ=255)    gCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGAtg                                                                                                                                             <  2:478614/151‑1 (MQ=255)                ccGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCccgc                                                                                                                                 >  1:286849/1‑151 (MQ=255)                  ggCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCccgccg                                                                                                                               >  2:765016/1‑151 (MQ=255)                     aaaaTCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGtt                                                                                                                             >  2:114009/1‑150 (MQ=255)                       aaTCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGATCAGTCTAGCACCACATGATGTTCGCGCTGTGCAGTAACGCTGTTCAGCGACGGATAGTTGGATGGGTTGCTCCCGCCGTTACg                                                                                                                          >  1:256542/1‑151 (MQ=255)                           gcgTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAAc                                                                                                                      >  2:146145/1‑151 (MQ=255)                            cgTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACt                                                                                                                     >  1:139460/1‑151 (MQ=255)                             gTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTg                                                                                                                    >  2:200271/1‑151 (MQ=255)                                cATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGgtt                                                                                                                 >  1:71940/1‑151 (MQ=255)                                 aTACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGgt                                                                                                                  >  2:338269/1‑149 (MQ=255)                                     aTCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTta                                                                                                             >  1:341933/1‑150 (MQ=255)                                          tttATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACg                                                                                                       >  2:278247/1‑151 (MQ=255)                                              tataATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGccc                                                                                                    >  2:233300/1‑150 (MQ=255)                                                   ttCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGtt                                                                                               >  1:532183/1‑150 (MQ=255)                                                    tCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTtat                                                                                             >  1:289218/1‑151 (MQ=255)                                                      ccTGTCCTGTTGCCCGCTACCGCTCCCGTACAGCGGGCCGGCGACCCCCACATGTTGTGCGCGCAGTGCAGTATCTTTATTCAGCGAAAAATAGTGGGTTGTGTTGCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAg                                                                                           <  1:1245/150‑1 (MQ=255)                                                            cTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGAAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTc                                                                                        >  2:632175/1‑148 (MQ=255)                                                               ttGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGAtt                                                                                   >  2:67864/1‑150 (MQ=255)                                                                   ccGCTACCTCACCCGTACCGGGAGCTGTCGACCACCACGTGATGTTCGCGCAGTGCATTACCTTTATTGCGCTACATATAGTGGGATTTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTAct                                                                                <  2:238990/149‑1 (MQ=255)                                                                           gATCCCGAACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCACGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCACAGATTACTGCTGAgg                                                                         >  2:421988/1‑148 (MQ=255)                                                                                        gAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGGTAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGTTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGGGTTAACACTGGAc                                                          >  1:49475/1‑150 (MQ=255)                                                                                           ccgccgacccccaattgattTTCGCGCAGTGAAGAAACTTTATTCGGCGAAAAATAGTGGGTTGTTTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGcc                                                      <  1:713128/136‑1 (MQ=255)                                                                                                      cacaTGATGTGCGCGGAGTGCAGTATTGTTATTCAGCGACAGATGTTTGGTTGTTTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGAt                                                  <  1:90364/144‑1 (MQ=255)                                                                                                         atgatgTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCt                                         >  2:280739/1‑150 (MQ=255)                                                                                                         atgatgTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCt                                         >  1:174321/1‑150 (MQ=255)                                                                                                                    ggggtgcagtATTGTTATGCGGCGAAAGATAGTGGGATGTTTTGCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAg                                  <  1:306537/143‑1 (MQ=255)                                                                                                                             tAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGcc                    >  2:98575/1‑151 (MQ=255)                                                                                                                              aaCGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGccc                   >  2:506418/1‑151 (MQ=255)                                                                                                                                     ttCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCtt            >  2:213003/1‑151 (MQ=255)                                                                                                                                      tCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTc           >  2:730758/1‑151 (MQ=255)                                                                                                                                               gATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTGAc  >  2:606722/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTGAC  >  NC_000913/308608‑308900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑