Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,567,770	G→A	A113V (GCG→GTG)	eutH ←	ethanolamine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,770	0	G	A	100.0%	176.5 / NA	46	A113V (GCG→GTG)	eutH	ethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/18);  total (28/18)

AATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCAT  >  NC_000913/2567622‑2567915                                                                                                                                                     |                                                                                                                                                  aaTGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCcgcccacc                                                                                                                                                 >  2:589058/1‑151 (MQ=255)            gCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGc                                                                                                                                      <  2:768189/151‑1 (MQ=255)                    aGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGga                                                                                                                                 >  2:474719/1‑148 (MQ=255)                     gcgcCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGgaagaa                                                                                                                             <  1:154385/151‑1 (MQ=255)                     gcgcCAGATAACGACGGTCAGAAGGTTCGATAATGCCGACCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGgaaga                                                                                                                              >  1:99521/1‑150 (MQ=255)                              aaCGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTGTGGCGAGGAAGAAGCCGCCCat                                                                                                                    <  2:54297/151‑1 (MQ=255)                               acgacgGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCat                                                                                                                    >  1:285575/1‑150 (MQ=255)                                 gacgGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATc                                                                                                                 >  2:688800/1‑151 (MQ=255)                                       cAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACg                                                                                                            <  2:552252/150‑1 (MQ=255)                                       cAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGc                                                                                                           >  2:610865/1‑151 (MQ=255)                                         gAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCca                                                                                                         <  2:630399/151‑1 (MQ=255)                                          aaGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGcc                                                                                                          >  1:7015/1‑149 (MQ=255)                                          aaGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCcag                                                                                                        <  2:10412/151‑1 (MQ=255)                                               tCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAgt                                                                                                    <  1:187540/150‑1 (MQ=255)                                                cGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAgtgt                                                                                                  >  1:249461/1‑151 (MQ=255)                                                 gATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt                                                                                                 >  2:514333/1‑151 (MQ=255)                                                  aTAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTc                                                                                                >  2:529774/1‑151 (MQ=255)                                                   tAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTcc                                                                                               >  2:242150/1‑151 (MQ=255)                                                    aaTGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCg                                                                                              <  1:280723/151‑1 (MQ=255)                                                    aaTGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCg                                                                                              >  2:180817/1‑151 (MQ=255)                                                         cGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGACGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGa                                                                                          >  2:628562/1‑150 (MQ=255)                                                           aGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAAc                                                                                        >  1:442756/1‑150 (MQ=255)                                                                 aCCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATg                                                                                 <  1:375906/151‑1 (MQ=255)                                                                           aaaaCACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATCTCGCACGCCAGCAGTGTTCCGGCGACCATCGATGGGTTAgcgc                                                                        <  1:466121/150‑1 (MQ=255)                                                                           aaaaCACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGcc                                                                       >  2:516644/1‑151 (MQ=255)                                                                                acaATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGc                                                                   >  2:504471/1‑150 (MQ=255)                                                                                    tCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTc                                                              >  1:771225/1‑151 (MQ=255)                                                                                      gTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGt                                                            <  2:467317/151‑1 (MQ=255)                                                                                       ttGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGTCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGt                                                            >  2:29828/1‑150 (MQ=255)                                                                                           ccccATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAAcc                                                       <  1:75484/151‑1 (MQ=255)                                                                                            cccATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCg                                                      <  1:688726/151‑1 (MQ=255)                                                                                               atcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCgg                                                     >  2:457586/1‑149 (MQ=255)                                                                                                tcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaa                                                   <  2:137435/150‑1 (MQ=255)                                                                                                tcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGCTAGCGCCGAGCATTTCGTAAACCgg                                                     >  1:184260/1‑148 (MQ=255)                                                                                                  atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaataa                                                <  1:140981/151‑1 (MQ=255)                                                                                                   tcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaataat                                               >  2:636265/1‑151 (MQ=255)                                                                                                   tcGACCCGAGAATTAACCCAGAGTATAGCCACGAGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaataat                                               >  2:524862/1‑151 (MQ=255)                                                                                                    cGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCg                                                      <  2:280033/143‑1 (MQ=255)                                                                                                    cGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCg                                                      >  1:280000/1‑143 (MQ=255)                                                                                                     gACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt                                              >  2:773839/1‑150 (MQ=255)                                                                                                               ttAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCg                                     >  1:484011/1‑149 (MQ=255)                                                                                                                 aaCCCAGAGTATAGCAACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCgaga                                  >  1:392077/1‑150 (MQ=255)                                                                                                                   cccAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACg                               >  2:604471/1‑151 (MQ=255)                                                                                                                           ataGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc                         >  2:355451/1‑149 (MQ=255)                                                                                                                                cACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCg                  <  2:279916/151‑1 (MQ=255)                                                                                                                                          cGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAgcgc        <  2:272119/151‑1 (MQ=255)                                                                                                                                           gTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGcgcg       >  2:229511/1‑151 (MQ=255)                                                                                                                                               ccgcccacCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTc    >  2:399651/1‑150 (MQ=255)                                                                                                                                                cgcccaccAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCAt  >  2:32771/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCAT  >  NC_000913/2567622‑2567915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑