Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,576,775	T→C	N535D (AAC→GAC)	maeB ←	malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,576,775	0	T	C	100.0%	175.9 / NA	49	N535D (AAC→GAC)	maeB	malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

CATGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCC  >  NC_000913/2576629‑2576920                                                                                                                                                   |                                                                                                                                                  cATGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTg                                                                                                                                               <  2:166194/151‑1 (MQ=255)      cACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAg                                                                                                                                          <  1:92425/151‑1 (MQ=255)       aCCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAgc                                                                                                                                         <  2:211144/151‑1 (MQ=255)             ggTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGc                                                                                                                                   >  1:518308/1‑151 (MQ=255)             ggTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGc                                                                                                                                   >  2:598134/1‑151 (MQ=255)                               aTAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTg                                                                                                                 <  1:530191/151‑1 (MQ=255)                               aTAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTg                                                                                                                 >  2:575000/1‑151 (MQ=255)                                   ccAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGAcgc                                                                                                             <  1:546465/151‑1 (MQ=255)                                        gACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCcgcg                                                                                                         >  2:223246/1‑150 (MQ=255)                                         aCATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGAc                                                                                                       <  2:49684/151‑1 (MQ=255)                                         aCATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGAc                                                                                                       <  2:165044/151‑1 (MQ=255)                                         aCATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGAc                                                                                                       >  1:578525/1‑151 (MQ=255)                                           aTTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCACCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGc                                                                                                     >  2:308367/1‑151 (MQ=255)                                             ttttCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCtt                                                                                                   >  1:655173/1‑151 (MQ=255)                                                  accacGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATg                                                                                               >  2:183108/1‑150 (MQ=255)                                                   ccacGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCATTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGAt                                                                                             <  2:769275/151‑1 (MQ=255)                                                   ccacGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCCTGAt                                                                                             >  1:323301/1‑151 (MQ=255)                                                      cGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTg                                                                                          <  2:714266/151‑1 (MQ=255)                                                            aaTGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGt                                                                                     >  1:713339/1‑150 (MQ=255)                                                                   aTGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTc                                                                             >  2:55752/1‑151 (MQ=255)                                                                       tAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGt                                                                         >  1:188939/1‑151 (MQ=255)                                                                             cccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActct                                                                    <  1:314978/150‑1 (MQ=255)                                                                              ccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActct                                                                    <  2:528844/149‑1 (MQ=255)                                                                                       cGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcgg                                                         >  1:235745/1‑151 (MQ=255)                                                                                          aaaTCATTGCATCGGCTTCCCCACGCTGAACCATCATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGCGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGGACTCTTTAAAGCGcgg                                                         >  1:62680/1‑148 (MQ=255)                                                                                             tCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcgg                                                    >  2:86027/1‑150 (MQ=255)                                                                                              cATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcggat                                                  >  2:671355/1‑151 (MQ=255)                                                                                                ttGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGAtt                                                 >  1:85152/1‑150 (MQ=255)                                                                                                 tGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTc                                                <  2:528662/150‑1 (MQ=255)                                                                                                  gCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGt                                              >  2:197998/1‑151 (MQ=255)                                                                                                   cATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGtt                                             >  2:192715/1‑151 (MQ=255)                                                                                                       ggCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTg                                         <  1:466880/151‑1 (MQ=255)                                                                                                            ccccACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAt                                    <  2:59410/151‑1 (MQ=255)                                                                                                            ccccACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAt                                    <  2:203194/151‑1 (MQ=255)                                                                                                              ccACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAtct                                  <  2:298355/151‑1 (MQ=255)                                                                                                              ccACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAtct                                  <  1:229201/151‑1 (MQ=255)                                                                                                               cACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAtctc                                 >  1:510485/1‑151 (MQ=255)                                                                                                                    tGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAAt                            >  2:399859/1‑151 (MQ=255)                                                                                                                     gAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAAt                            <  1:759516/150‑1 (MQ=255)                                                                                                                       aCCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCa                          >  2:211334/1‑150 (MQ=255)                                                                                                                           tGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGcc                     >  2:288482/1‑151 (MQ=255)                                                                                                                               cgcgCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCt                 <  1:673136/151‑1 (MQ=255)                                                                                                                                  gcCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTg              >  2:741771/1‑151 (MQ=255)                                                                                                                                      aTCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCt          <  2:358118/151‑1 (MQ=255)                                                                                                                                        cACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGc        <  1:613782/151‑1 (MQ=255)                                                                                                                                            gTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCaa      <  2:713985/149‑1 (MQ=255)                                                                                                                                            gTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGc    <  1:557061/151‑1 (MQ=255)                                                                                                                                                ggTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGccc  >  2:441836/1‑149 (MQ=255)                                                                                                                                                ggTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGccc  >  2:270009/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CATGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCC  >  NC_000913/2576629‑2576920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑