Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,723	G→A	R302R (CGG→CGA)	hyfB →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,723	0	G	A	100.0%	162.0 / NA	45	R302R (CGG→CGA)	hyfB	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/26);  total (19/26)

GTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCT  >  NC_000913/2602578‑2602866                                                                                                                                                  |                                                                                                                                                gTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGactgct                                                                                                                                            >  2:223738/1‑151 (MQ=255)         ggCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGa                                                                                                                                    <  1:470255/151‑1 (MQ=255)          gCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAg                                                                                                                                   <  1:168380/151‑1 (MQ=255)            aTCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTa                                                                                                                                 <  2:584324/151‑1 (MQ=255)                 gAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGGTCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTGCCGTc                                                                                                                            <  1:63300/151‑1 (MQ=255)                 gAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCg                                                                                                                              <  2:421478/149‑1 (MQ=255)                 gAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTc                                                                                                                            >  1:29600/1‑151 (MQ=255)                            tGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCg                                                                                                                  >  1:727613/1‑150 (MQ=255)                             ggctCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGGTGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCGGGCCTGGAGGACCGTCGAAAACg                                                                                                                     >  1:68219/4‑146 (MQ=255)                              gATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGc                                                                                                                >  1:168068/1‑150 (MQ=255)                                 ctgctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCatt                                                                                                             >  2:87946/1‑150 (MQ=255)                                   gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCattatt                                                                                                          <  1:76931/151‑1 (MQ=255)                                   gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCattat                                                                                                           <  2:615454/150‑1 (MQ=255)                                   gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCatt                                                                                                             <  2:303543/148‑1 (MQ=255)                                       gcgcAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGc                                                                                                      >  1:579654/1‑151 (MQ=255)                                          cAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTgg                                                                                                   >  1:435930/1‑151 (MQ=255)                                            aaCGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCa                                                                                                 <  1:253378/151‑1 (MQ=255)                                               gggTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTc                                                                                              >  1:449649/1‑151 (MQ=255)                                                   ttGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGgtg                                                                                          >  1:285467/1‑151 (MQ=255)                                                     gCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGgtgt                                                                                         <  2:234223/150‑1 (MQ=255)                                                             ggtggGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATgg                                                                                 <  2:459603/150‑1 (MQ=255)                                                                ggggCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATggtcg                                                                              <  2:493430/150‑1 (MQ=255)                                                                 gggCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGGCGGTGTGGAGATggt                                                                                <  1:448852/147‑1 (MQ=255)                                                                  ggCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATggtcggtc                                                                           <  2:291821/151‑1 (MQ=255)                                                                         tGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACt                                                                    >  2:230674/1‑151 (MQ=255)                                                                             gATGGAGATCGTCGCAATCTCCGCGCTCCTGGGCGAGCTATATGAGCTGGCGGGACAGGATATCAAACGACTGCTGGCCTGGTGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTAGGTCTGTCACTGctc                                                                >  1:522540/1‑149 (MQ=255)                                                                                  cGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGAccc                                                           >  1:118754/1‑151 (MQ=255)                                                                                  cGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGAccc                                                           >  2:411886/1‑151 (MQ=255)                                                                                   gATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCg                                                          <  2:266311/151‑1 (MQ=255)                                                                                   gATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCg                                                          >  2:750431/1‑151 (MQ=255)                                                                                   gATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCg                                                          >  1:307993/1‑151 (MQ=255)                                                                                          gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCa                                                   <  1:718143/151‑1 (MQ=255)                                                                                          gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCa                                                   <  2:435981/151‑1 (MQ=255)                                                                                                        ccTGGGCGCGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTg                                     >  2:230276/1‑151 (MQ=255)                                                                                                            ggCGTGCTATATGCGCTGGCGGGACAGGATATCAAACGACTGCTGGCCTGTAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCt                                   <  2:617997/149‑1 (MQ=255)                                                                                                             gCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCgg                                <  2:278875/151‑1 (MQ=255)                                                                                                              cGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGgc                               >  2:427324/1‑151 (MQ=255)                                                                                                              cGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGgc                               <  1:566412/151‑1 (MQ=255)                                                                                                                   tataTGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                          >  2:489890/1‑151 (MQ=255)                                                                                                                   tataTGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                          >  2:96519/1‑151 (MQ=255)                                                                                                                    atatGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                          <  2:614107/150‑1 (MQ=255)                                                                                                                    atatGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTg                         <  1:75022/151‑1 (MQ=255)                                                                                                                      atGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGt                        <  1:740721/150‑1 (MQ=255)                                                                                                                                       gATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATg      <  2:100523/151‑1 (MQ=255)                                                                                                                                            cAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCTCGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCt  <  2:412715/150‑1 (MQ=255)                                                                                                                                            cAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCt  <  2:183399/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                GTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCT  >  NC_000913/2602578‑2602866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑