Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,708,301	T→C	T152A (ACA→GCA)	rseB ←	anti‑sigma E factor, binds RseA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,708,301	0	T	C	100.0%	217.5 / NA	58	T152A (ACA→GCA)	rseB	anti‑sigma E factor, binds RseA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/28);  total (30/28)

CTGCATACTGCTGCTGATATCCTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGTACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATCAACAATGTAATCGCC  >  NC_000913/2708158‑2708445                                                                                                                                                |                                                                                                                                                 cTGCATACTGCTGCTGATATCCTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCAtct                                                                                                                                           >  1:213246/1‑151 (MQ=255) cTGCATACTGCTGCTGATATCCTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCAtc                                                                                                                                            >  2:294904/1‑150 (MQ=255) cTGCATACTGCTGCTGATATCCTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCAt                                                                                                                                             >  2:383819/1‑149 (MQ=255)                gATATCCTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCAcgcg                                                                                                                             >  2:218522/1‑150 (MQ=255)                    tCCTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATg                                                                                                                         >  1:737915/1‑150 (MQ=255)                      cTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACt                                                                                                                      >  1:603318/1‑151 (MQ=255)                             aCGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCaa                                                                                                                >  2:740370/1‑150 (MQ=255)                                  aaaaGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACg                                                                                                           <  2:312612/150‑1 (MQ=255)                                   aaaGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGAt                                                                                                         <  1:607711/151‑1 (MQ=255)                                                gAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGt                                                                                            >  1:182609/1‑151 (MQ=255)                                                       ttCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCg                                                                                     >  1:155772/1‑151 (MQ=255)                                                         ccAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGAc                                                                                   <  2:155715/151‑1 (MQ=255)                                                          cAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACg                                                                                  <  1:673970/151‑1 (MQ=255)                                                            gCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACgg                                                                                 >  2:259683/1‑150 (MQ=255)                                                            gCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGga                                                                                <  2:129070/151‑1 (MQ=255)                                                             cGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACgg                                                                                 >  2:356631/1‑149 (MQ=255)                                                               tttCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACgg                                                                                 >  1:370518/1‑147 (MQ=255)                                                                ttCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGAt                                                                             >  1:473406/1‑150 (MQ=255)                                                                ttCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGAt                                                                             <  1:75794/150‑1 (MQ=255)                                                                  cACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATaa                                                                           >  2:506205/1‑150 (MQ=255)                                                                   aCCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATaa                                                                           >  1:441540/1‑149 (MQ=255)                                                                        cgcgATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCgta                                                                    <  1:364363/151‑1 (MQ=255)                                                                          cgATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCgtagt                                                                  <  2:155805/151‑1 (MQ=255)                                                                              cAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGCAGTAAgg                                                              >  1:192701/1‑151 (MQ=255)                                                                               aaGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGCATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAgg                                                              >  1:476160/1‑150 (MQ=255)                                                                               aaGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAgg                                                              >  1:647858/1‑150 (MQ=255)                                                                                  gagaTCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGaa                                                          <  2:705291/151‑1 (MQ=255)                                                                                  gagaTCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGaa                                                          <  2:69397/151‑1 (MQ=255)                                                                                   agaTCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGgagac                                                          >  1:396149/1‑149 (MQ=255)                                                                                   agaTCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGaa                                                          >  1:393415/1‑150 (MQ=255)                                                                                    gaTCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATGTCGGGCAACCACGCGAATGACTTCGAAAAGACGATGAGCAATACGCGTGCGCCAGACGGAGATAAAGTCGTAGTAAAgaca                                                           <  1:443531/148‑3 (MQ=255)                                                                                    gaTCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAg                                                        <  2:335052/151‑1 (MQ=255)                                                                                     aTCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAgg                                                       <  2:57479/151‑1 (MQ=255)                                                                                       cAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCg                                                     <  2:541915/151‑1 (MQ=255)                                                                                          cccGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGttt                                                  <  1:285224/151‑1 (MQ=255)                                                                                            cGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGa                                                <  1:616298/151‑1 (MQ=255)                                                                                             gCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGaa                                               <  1:298269/151‑1 (MQ=255)                                                                                              cATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGaaa                                              <  2:526885/151‑1 (MQ=255)                                                                                               aTCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAAt                                             <  1:36698/151‑1 (MQ=255)                                                                                                     aaTTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGtat                                       <  2:283277/151‑1 (MQ=255)                                                                                                     aaTTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGtat                                       >  2:569317/1‑151 (MQ=255)                                                                                                       tttCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGtat                                       >  1:554156/1‑149 (MQ=255)                                                                                                          cGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGAt                                   <  1:729964/150‑1 (MQ=255)                                                                                                           gATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGta                                        >  2:193593/1‑144 (MQ=255)                                                                                                           gATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGta                                        <  1:193560/144‑1 (MQ=255)                                                                                                            aTTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAg                                <  1:296985/151‑1 (MQ=255)                                                                                                              tCGGTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCg                              >  1:298267/1‑151 (MQ=255)                                                                                                                ggTGTCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGAt                            <  1:322406/151‑1 (MQ=255)                                                                                                                  tgtCCATCCACACGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATgg                          >  1:316369/1‑151 (MQ=255)                                                                                                                            cacGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATca                <  2:53163/151‑1 (MQ=255)                                                                                                                             acGATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATca                >  1:341422/1‑150 (MQ=255)                                                                                                                               gATGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATcaac              >  1:156819/1‑150 (MQ=255)                                                                                                                                aTGTAGCTGTAGCGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATcaaca             >  2:430816/1‑150 (MQ=255)                                                                                                                                  gtagctgtagcGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATCAACAAt           <  2:121391/150‑1 (MQ=255)                                                                                                                                    agctgtagcGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATCAACAATGTa        <  2:528117/151‑1 (MQ=255)                                                                                                                                    agctgtagcGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATCAACAATGTa        <  2:243110/151‑1 (MQ=255)                                                                                                                                         tagcGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATCAACAATGTAATCGc   >  2:467824/1‑151 (MQ=255)                                                                                                                                           gcGTGCACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATCAACAATGTAATCGcc  >  2:729008/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                 CTGCATACTGCTGCTGATATCCTGATTGACGTTAAAAGCAATCACGCGAAATTGTTCCAGCGTTTCACCATCGCGATCAAGGAGATCAACCCGCATCGGTAATTTCGATTCGGTGTCCATCCACACGATGTAGCTGTAGCGTGTACCATCTCGGGCAACCACGCGAATGACTTCGCAAAGACGATCAGCAATACGCGTGCGCCCGACGGAGATAAAGTCGTAGTAAGGAGAAAGGCGTTTGAAATCGGTATAGATAAGCGATGGCAGAGAATCAACAATGTAATCGCC  >  NC_000913/2708158‑2708445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑