Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	325,803	T→C	Y482C (TAC→TGC)	betA ←	choline dehydrogenase, a flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	325,803	0	T	C	100.0%	170.4 / NA	48	Y482C (TAC→TGC)	betA	choline dehydrogenase, a flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

ATCATATCCGCTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGTAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAGCGCGGGTTGATGCATGAT  >  NC_000913/325656‑325948                                                                                                                                                    |                                                                                                                                                  aTCATATCCGCTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAAc                                                                                                                                                >  2:90052/1‑151 (MQ=255)   cATATCCGCTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAAcc                                                                                                                                               <  2:483613/150‑1 (MQ=255)    atatCCGCTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAAccc                                                                                                                                              >  2:114492/1‑150 (MQ=255)         cGCTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTg                                                                                                                                        >  1:703500/1‑151 (MQ=255)          gCTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGc                                                                                                                                       <  2:601319/151‑1 (MQ=255)           cTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGc                                                                                                                                       >  2:549107/1‑150 (MQ=255)           cTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGc                                                                                                                                       >  2:460780/1‑150 (MQ=255)              ttttCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGt                                                                                                                                   >  2:66285/1‑151 (MQ=255)                ttCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTa                                                                                                                                  <  2:482984/150‑1 (MQ=255)                 tctcGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTAc                                                                                                                                 <  2:486964/150‑1 (MQ=255)                    cGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCg                                                                                                                               <  1:496205/149‑1 (MQ=255)                      ccAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGTATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACg                                                                                                                           <  2:396776/151‑1 (MQ=255)                        aaTCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGa                                                                                                                         <  2:458495/151‑1 (MQ=255)                           cATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATg                                                                                                                       <  2:255101/150‑1 (MQ=255)                             tAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGa                                                                                                                     >  1:585546/1‑150 (MQ=255)                              aaTTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGa                                                                                                                     >  2:105652/1‑149 (MQ=255)                              aaTTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAg                                                                                                                   <  2:532323/151‑1 (MQ=255)                                    cGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGtt                                                                                                             <  1:158751/151‑1 (MQ=255)                                    cGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGt                                                                                                              <  1:515730/150‑1 (MQ=255)                                          gTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCg                                                                                                       <  2:675731/151‑1 (MQ=255)                                           ttCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCg                                                                                                       >  2:202284/1‑150 (MQ=255)                                            tCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTg                                                                                                     >  2:634420/1‑151 (MQ=255)                                              aaaTTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGt                                                                                                   >  2:538006/1‑151 (MQ=255)                                               aaTTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGtt                                                                                                  >  2:742146/1‑151 (MQ=255)                                                                tGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCg                                                                                  >  1:192345/1‑150 (MQ=255)                                                                     cATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGt                                                                            >  1:505873/1‑151 (MQ=255)                                                                       tAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATTTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTc                                                                          >  1:646312/1‑151 (MQ=255)                                                                        aaTCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTc                                                                          >  2:662990/1‑150 (MQ=255)                                                                           cGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATcc                                                                      <  1:6347/151‑1 (MQ=255)                                                                           cGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATcc                                                                      >  2:372755/1‑151 (MQ=255)                                                                            gACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTg                                                                             >  1:8939/1‑143 (MQ=255)                                                                            gACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTg                                                                             <  2:8967/143‑1 (MQ=255)                                                                                 aTCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTgg                                                                <  1:640782/151‑1 (MQ=255)                                                                                   ccaccaCACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGc                                                               <  1:508478/150‑1 (MQ=255)                                                                                     accacACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCAtt                                                            >  1:560918/1‑151 (MQ=255)                                                                                            gCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACAcc                                                     <  1:193438/151‑1 (MQ=255)                                                                                             cAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCg                                                    <  2:628929/151‑1 (MQ=255)                                                                                              aGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCgg                                                   <  2:611624/151‑1 (MQ=255)                                                                                                          ccGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTcgc                                       >  1:159952/1‑151 (MQ=255)                                                                                                                   cgGCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGAt                              <  1:513712/151‑1 (MQ=255)                                                                                                                    ggCCTTCGCCGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTGCGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATa                             <  2:141955/151‑1 (MQ=255)                                                                                                                            ccGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCa                     <  1:76052/151‑1 (MQ=255)                                                                                                                             cGTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATcc                      >  2:732678/1‑149 (MQ=255)                                                                                                                              gTCAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAgc                   <  2:192378/151‑1 (MQ=255)                                                                                                                                cAACCACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAgcgc                 >  2:277145/1‑151 (MQ=255)                                                                                                                                    cACGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAGCGCGGGt             <  2:190313/151‑1 (MQ=255)                                                                                                                                      cGGACATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAGCGCGGGTTg           <  1:702809/151‑1 (MQ=255)                                                                                                                                         aCATCTCGTCGCAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAGCGCGGGTTGATg        >  2:247207/1‑151 (MQ=255)                                                                                                                                               cgtcgcAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAGCGCGGGTTGATGCATGa   <  1:318188/150‑1 (MQ=255)                                                                                                                                               cgtcgcAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAGCGCGGGTTGATGCATGAt  >  1:165359/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ATCATATCCGCTATTTTCTCGCCAATCATAATTGTCGTGGCGTTCAAATTCCCGGTGATAATCTGCGGCATAATCGACGCATCCACCACACGCAGGCCTTCTAACCCGTGTACGCGGCCTTCGCCGTCAACCACGGACATCTCGTCGTAACCCATTTTGCAGGTACCGCACGGATGGAAGGCGGTTTCGGCGTGGTTACGCACGAACTCATCGAGCTGTTCATCCGTCTGGCATTCGACACCGGGGCTGATTTCGCGGCCACGATACTGATCCAGCGCGGGTTGATGCATGAT  >  NC_000913/325656‑325948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑