Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,738,658	G→A	G305E (GGG→GAG)	pheA →	chorismate mutase and prephenate dehydratase, P‑protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,738,658	0	G	A	100.0%	199.4 / NA	53	G305E (GGG→GAG)	pheA	chorismate mutase and prephenate dehydratase, P‑protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/26);  total (27/26)

CTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGG  >  NC_000913/2738511‑2738804                                                                                                                                                    |                                                                                                                                                   cTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGc                                                                                                                                                  >  1:76689/1‑150 (MQ=255) cTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGc                                                                                                                                                  >  1:595444/1‑150 (MQ=255)   ggcggcACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGcaa                                                                                                                                                <  2:73257/150‑1 (MQ=255)    gcggcACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGcaaca                                                                                                                                              <  1:669008/151‑1 (MQ=255)                aCGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCt                                                                                                                                  >  1:410753/1‑151 (MQ=255)                       gCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGa                                                                                                                            <  1:305069/150‑1 (MQ=255)                        cAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAg                                                                                                                          <  1:384071/151‑1 (MQ=255)                             aCTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTGCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGtt                                                                                                                      <  1:152586/150‑1 (MQ=255)                                ggAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTg                                                                                                                  <  2:151178/151‑1 (MQ=255)                                ggAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTg                                                                                                                  >  1:257665/1‑151 (MQ=255)                                 gAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTgg                                                                                                                 >  1:304324/1‑151 (MQ=255)                                     gTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACt                                                                                                             <  2:275058/151‑1 (MQ=255)                                       aTTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTgc                                                                                                           >  2:141199/1‑151 (MQ=255)                                       aTTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTg                                                                                                            <  2:417927/150‑1 (MQ=255)                                         tGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTgcgc                                                                                                         >  1:549269/1‑151 (MQ=255)                                                 aTCAGCGACAAAACTTCGCCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACaa                                                                                                 <  1:257242/151‑1 (MQ=255)                                                        aCAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGAtt                                                                                          >  1:126464/1‑151 (MQ=255)                                                        aCAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGAtt                                                                                          >  2:731966/1‑151 (MQ=255)                                                            aaCTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATg                                                                                       >  1:441246/1‑150 (MQ=255)                                                            aaCTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATg                                                                                       >  2:111628/1‑150 (MQ=255)                                                                 cACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGt                                                                                 >  2:321404/1‑151 (MQ=255)                                                                 cACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGt                                                                                 <  1:288659/151‑1 (MQ=255)                                                                        tttGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGAccc                                                                                   >  1:595765/1‑142 (MQ=255)                                                                        tttGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGAccc                                                                                   <  2:595822/142‑1 (MQ=255)                                                                           gtggtgTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCAc                                                                       >  2:243767/1‑151 (MQ=255)                                                                           gtggtgTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCAc                                                                       <  1:24768/151‑1 (MQ=255)                                                                               tgtTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGccc                                                                   >  2:337450/1‑151 (MQ=255)                                                                                       cgTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACg                                                           >  1:652457/1‑151 (MQ=255)                                                                                          aaaGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGt                                                         >  2:2401/1‑150 (MQ=255)                                                                                                  tAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGTTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATg                                                 <  2:767934/150‑1 (MQ=255)                                                                                                  tAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATgg                                                >  2:120424/1‑151 (MQ=255)                                                                                                  tAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATgg                                                >  2:139777/1‑151 (MQ=255)                                                                                                   aaCGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGATGCGCTGGTTGAAGCGTTGCTATTACTGCGCAGCCATAATCTGAAAATGACCCGTCTGGAATCACGCCCGATTCACGGTAAGCCATg                                                 <  1:154903/149‑1 (MQ=255)                                                                                                    aCGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCCCGCCCGATTCACGGTAATCCAt                                                  <  1:418793/147‑1 (MQ=255)                                                                                                     cGTGTCTGATCAGGTTCCGGCGAAAACTACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGa                                              >  2:199308/1‑150 (MQ=255)                                                                                                     cGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGa                                              >  1:150919/1‑150 (MQ=255)                                                                                                        gtCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAaga                                           <  1:236055/150‑1 (MQ=255)                                                                                                             aTCAGGTCCCGGCGAAACCCCCGTTGTTAATGGCGCCCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGTTACTGCGCAACCACAATCTTATTATGACCCGTCTGGAATCCCGCCCGATTCACGGTAATCCATGTGAAGAGATg                                       <  1:776838/149‑1 (MQ=255)                                                                                                                aGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTa                                  <  2:598298/151‑1 (MQ=255)                                                                                                                       ggCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGat                           >  1:9874/1‑151 (MQ=255)                                                                                                                       ggCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGat                           <  1:139744/151‑1 (MQ=255)                                                                                                                       ggCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGat                           <  1:210449/151‑1 (MQ=255)                                                                                                                        gCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGata                          <  1:774552/151‑1 (MQ=255)                                                                                                                        gCGAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGat                           >  1:737722/1‑150 (MQ=255)                                                                                                                          gAAAACCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGatat                         <  2:732001/150‑1 (MQ=255)                                                                                                                           aaaaCCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTc                       >  1:112385/1‑151 (MQ=255)                                                                                                                            aaaCCACGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTc                       >  2:735814/1‑150 (MQ=255)                                                                                                                                  cGTTGTTAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa                 <  2:454947/150‑1 (MQ=255)                                                                                                                                      gttAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCt             <  1:632949/150‑1 (MQ=255)                                                                                                                                       ttAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGaa                                             <  2:394759/117‑1 (MQ=255)                                                                                                                                       ttAATGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGaa                                             >  1:394710/1‑117 (MQ=255)                                                                                                                                           tGGCGACCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATc       >  1:316959/1‑151 (MQ=255)                                                                                                                                                aCCGAGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCgg  <  2:202426/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGG  >  NC_000913/2738511‑2738804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑