Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,807,897	A→G	T256A (ACC→GCC)	proX →	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,807,897	0	A	G	100.0%	173.6 / NA	48	T256A (ACC→GCC)	proX	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

ACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGA  >  NC_000913/2807751‑2808036                                                                                                                                                   |                                                                                                                                            acacCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCAt                                                                                                                                         <  2:273670/151‑1 (MQ=255)      tGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCat                                                                                                                                     >  1:423867/1‑150 (MQ=255)           gCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGtt                                                                                                                               <  2:188941/151‑1 (MQ=255)           gCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGtt                                                                                                                               >  1:259515/1‑151 (MQ=255)            ccGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTg                                                                                                                              >  1:285607/1‑151 (MQ=255)              gTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCg                                                                                                                                 >  1:728569/1‑146 (MQ=255)              gTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCg                                                                                                                                 <  2:728643/146‑1 (MQ=255)               tACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGcc                                                                                                                            >  1:743955/1‑150 (MQ=255)                aCTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCca                                                                                                                           <  2:19069/150‑1 (MQ=255)                   gggTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCcaaca                                                                                                                        >  2:762343/1‑150 (MQ=255)                       gcgTAACGAACGGAAGCCGGGCAAAGATGTCGTCTGGTTGCCGGTGCCGTTCTCCGCACTGCCGGGCGATACACACGCCGGTACCAAACCCGCGAATGGCGCGAATTCTGGCTTCCCGGTCAGCGACCTGCATCTCGTTGCCAACAAAccc                                                                                                                   <  1:2927/149‑1 (MQ=255)                        aGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCt                                                                                                                  >  1:267175/1‑151 (MQ=255)                         gTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTg                                                                                                                 <  1:706862/151‑1 (MQ=255)                          tAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTgg                                                                                                                >  2:416961/1‑151 (MQ=255)                              gAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCg                                                                                                            <  2:750296/151‑1 (MQ=255)                                   gAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGaa                                                                                                        <  1:666354/150‑1 (MQ=255)                                         gggCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCg                                                                                                 <  1:71593/151‑1 (MQ=255)                                             aaaGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAACCTGGGCCGAGAAAAACCCGgc                                                                                               >  2:243738/1‑149 (MQ=255)                                              aaGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGgcagc                                                                                            <  2:262463/151‑1 (MQ=255)                                               aGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGgcagca                                                                                           <  1:505979/151‑1 (MQ=255)                                                 aTGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACTAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCagcagc                                                                                         >  1:222196/1‑151 (MQ=255)                                                      gtcTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAAc                                                                                    >  2:330815/1‑151 (MQ=255)                                                               cAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCa                                                                           <  2:153266/151‑1 (MQ=255)                                                                aGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCAt                                                                          <  2:479609/151‑1 (MQ=255)                                                                    gCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTAt                                                                       <  1:596332/150‑1 (MQ=255)                                                                    gCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATg                                                                      <  1:32500/151‑1 (MQ=255)                                                                     ccGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGc                                                                     <  2:110384/151‑1 (MQ=255)                                                                     ccGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGc                                                                     >  2:315771/1‑151 (MQ=255)                                                                          ctcCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTg                                                                >  2:474329/1‑151 (MQ=255)                                                                            ccGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGtt                                                                 <  1:746517/148‑1 (MQ=255)                                                                             cGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGcc                                                              >  1:69248/1‑150 (MQ=255)                                                                                aCTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTg                                                          >  1:677176/1‑151 (MQ=255)                                                                                 cTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTgg                                                         >  2:330390/1‑151 (MQ=255)                                                                                   gCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCa                                                       <  1:749842/151‑1 (MQ=255)                                                                                           aTAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTa                                                >  1:171192/1‑150 (MQ=255)                                                                                              aaaaCGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGc                                            >  2:219745/1‑151 (MQ=255)                                                                                              aaaaCGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGc                                            >  1:290275/1‑151 (MQ=255)                                                                                                 aCGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCa                                         <  2:631758/151‑1 (MQ=255)                                                                                                     cGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAAc                                     >  2:50914/1‑151 (MQ=255)                                                                                                      gATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACg                                    >  1:658650/1‑151 (MQ=255)                                                                                                                 gCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCAt                         <  1:377045/151‑1 (MQ=255)                                                                                                                  ccGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATg                        <  2:491543/151‑1 (MQ=255)                                                                                                                       tGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGc                   >  1:21698/1‑151 (MQ=255)                                                                                                                                  aTGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTc           >  1:583307/1‑148 (MQ=255)                                                                                                                                    ggCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGa        >  2:126948/1‑149 (MQ=255)                                                                                                                                        tCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGc    <  2:186354/149‑1 (MQ=255)                                                                                                                                        tCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGc    <  1:219713/149‑1 (MQ=255)                                                                                                                                         cccGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGa  <  2:696345/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            ACACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGA  >  NC_000913/2807751‑2808036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑