Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,577	A→G	V812A (GTT→GCT)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,577	0	A	G	100.0%	144.2 / NA	40	V812A (GTT→GCT)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/25);  total (15/25)

AGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTT  >  NC_000913/2819429‑2819722                                                                                                                                                     |                                                                                                                                                  aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCTCCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                 <  2:444295/151‑1 (MQ=255) aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                 <  1:14175/151‑1 (MQ=255) aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                 >  2:254106/1‑151 (MQ=255)                aTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTAcc                                                                                                                                  >  1:364576/1‑151 (MQ=255)                     tACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGc                                                                                                                             >  1:83939/1‑151 (MQ=255)                           cGGCTTGCGCCAGGTCAGGACGTCCACCCCCCTTGCCGCCCACCTGCTGAGCGACCATAACAATCAGTTCCCCTGCTTTCCCACGATCTGTGACGTCCTTAGATACGCCTGCAAACAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCAc                                                                                                                       <  1:405114/151‑1 (MQ=255)                             gCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGa                                                                                                                     <  2:703636/151‑1 (MQ=255)                                 gcgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATaa                                                                                                                  <  2:598232/150‑1 (MQ=255)                                 gcgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAAt                                                                                                                 <  1:254073/151‑1 (MQ=255)                                 gcgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAAt                                                                                                                 >  2:201551/1‑151 (MQ=255)                                  cgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAAtt                                                                                                                <  2:720686/151‑1 (MQ=255)                                   gcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTg                                                                                                               >  2:754266/1‑151 (MQ=255)                                     catctcattaCGACCACCACCCCTTCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACCAGCCCTGACGTCCTCAGATACGCCTACAATCAGAGAAGCCGTTCCTTCGACTACCGTTGCCTGCACGATAATTGtt                                                                                                             <  1:542856/142‑2 (MQ=255)                                            ggACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCa                                                                                                      <  2:258607/151‑1 (MQ=255)                                                  ccaccaCCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAt                                                                                                <  2:210495/151‑1 (MQ=255)                                                   caccacCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtt                                                                                               <  1:386384/151‑1 (MQ=255)                                                   caccacCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtt                                                                                               >  1:40946/1‑151 (MQ=255)                                                    accaccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAttt                                                                                              <  1:549229/151‑1 (MQ=255)                                                     ccaccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtttt                                                                                             <  2:47127/151‑1 (MQ=255)                                                      caccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAttttt                                                                                            >  1:217884/1‑151 (MQ=255)                                                        cccTTGCCGCCCACCTGCTGAGCGACCATACCAAGCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTa                                                                                          <  2:483892/151‑1 (MQ=255)                                                                 cccACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCa                                                                                  >  1:418377/1‑150 (MQ=255)                                                                  ccACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAc                                                                                <  1:4646/151‑1 (MQ=255)                                                                          tgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTa                                                                         >  1:602574/1‑150 (MQ=255)                                                                                 ccATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACAt                                                                 <  2:289421/151‑1 (MQ=255)                                                                                 ccATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACAt                                                                 >  2:359907/1‑151 (MQ=255)                                                                                  cATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACAtt                                                                >  1:100462/1‑151 (MQ=255)                                                                                                  ccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGc                                                <  2:187442/151‑1 (MQ=255)                                                                                                     gCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTa                                              <  2:552727/150‑1 (MQ=255)                                                                                                       tttCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGc                                           >  2:208787/1‑151 (MQ=255)                                                                                                         tCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTc                                         >  2:734892/1‑151 (MQ=255)                                                                                                          cacaCGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTc                                         <  2:273215/150‑1 (MQ=255)                                                                                                            cacGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCt                                      <  1:602941/151‑1 (MQ=255)                                                                                                            cacGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCt                                      <  2:29851/151‑1 (MQ=255)                                                                                                                  cTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCa                                 >  2:435169/1‑150 (MQ=255)                                                                                                                    gtgACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACa                              <  1:490909/151‑1 (MQ=255)                                                                                                                      gACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGc                            <  2:137957/151‑1 (MQ=255)                                                                                                                       aCGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATGTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGc                            <  1:342471/150‑1 (MQ=255)                                                                                                                                gATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCAt                  <  2:689810/151‑1 (MQ=255)                                                                                                                                   aCGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTa                <  2:269464/150‑1 (MQ=255)                                                                                                                                   aCGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTa                >  1:482629/1‑150 (MQ=255)                                                                                                                                      ccTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACAt            >  1:189601/1‑151 (MQ=255)                                                                                                                                                gagaAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCtt  <  1:548597/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTT  >  NC_000913/2819429‑2819722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑