BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,849,543	G→A	R103C (CGT→TGT)	hycB ←	hydrogenase 3, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,543	0	G	A	100.0%	120.9 / NA	34	R103C (CGT→TGT)	hycB	hydrogenase 3, Fe‑S subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/13); total (21/13)

ATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTT  >  NC_000913/2849403‑2849686

atcaAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGa                                                                                                                                         >  2:598887/1‑150 (MQ=255)

        tACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTTGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCaa                                                                                                                                 >  1:693710/1‑150 (MQ=255)

         aCAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAAtt                                                                                                                               <  1:595897/151‑1 (MQ=255)

          cAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTg                                                                                                                              <  1:374981/151‑1 (MQ=255)

          cAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCCGGGGATTGGCGTTTTCCGGAATATCCAGCGGACACCTGCCGGAAAATTCAAtt                                                                                                                               >  1:333510/1‑150 (MQ=255)

                    aTTTGAAGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGAGCCGGTGGGGCTTTAGGGGGATTGGGGTTTGCCGGAATATCCAGCGGACAGATGCCGGAAAATTCAATTGCG‑CCAAACg                                                                                                                    >  1:605561/1‑150 (MQ=255)

                     tttGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACggg                                                                                                                  >  2:419450/1‑151 (MQ=255)

                             cGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGa                                                                                                           >  2:512777/1‑150 (MQ=255)

                              gATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCCTTCGGGGTATTCGCGTTTGCCGGGATTTCCAGCGGACAGATGCCGGCAAATTCAATTGCGCCCAAACGGCCAgcggtg                                                                                                          >  1:145691/1‑146 (MQ=255)

                                  gcgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGcc                                                                                                       >  1:476049/1‑149 (MQ=255)

                                   cgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGca                                                                                                    <  2:575363/151‑1 (MQ=255)

                                   cgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGc                                                                                                     >  2:366748/1‑150 (MQ=255)

                                      gAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGcaca                                                                                                  <  1:667242/150‑1 (MQ=255)

                                         tACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGGTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGcac                                                                                                   >  1:456310/1‑146 (MQ=255)

                                            cTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTg                                                                                             >  1:633476/1‑149 (MQ=255)

                                                         aaGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCCGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGcaca                                                                                >  1:447423/1‑149 (MQ=255)

                                                           gCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCt                                                                            <  2:397908/151‑1 (MQ=255)

                                                              aTGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttc                                                                         <  1:421455/151‑1 (MQ=255)

                                                                  gCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcattc                                                                     >  2:746118/1‑151 (MQ=255)

                                                                  gCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcatt                                                                      >  1:477538/1‑150 (MQ=255)

                                                                         cgcgCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCa                                                              <  1:373891/151‑1 (MQ=255)

                                                                            gcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACgg                                                           <  1:51953/151‑1 (MQ=255)

                                                                            gcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACgg                                                           >  2:710730/1‑151 (MQ=255)

                                                                                 aGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGcccc                                                       >  2:212730/1‑150 (MQ=255)

                                                                                    aGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCg                                                   >  1:202980/1‑151 (MQ=255)

                                                                                         gTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGAcgcg                                              >  2:197837/1‑151 (MQ=255)

                                                                                                       ttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGtt                                  <  1:615507/149‑1 (MQ=255)

                                                                                                       ttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGtt                                  <  2:21036/149‑1 (MQ=255)

                                                                                                          ggggTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCg                             <  1:450290/151‑1 (MQ=255)

                                                                                                              tATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc                          >  1:366436/1‑150 (MQ=255)

                                                                                                               aTTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc                          >  1:267320/1‑149 (MQ=255)

                                                                                                                       ttGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAAACGCGCAGACCACcg                 <  1:431519/150‑1 (MQ=255)

                                                                                                                               atatCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTg        <  1:128401/151‑1 (MQ=255)

                                                                                                                                     aGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCtt  >  1:490406/1‑151 (MQ=255)

ATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTT  >  NC_000913/2849403‑2849686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`