Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,791	T→C	Y153H (TAT→CAT)	ygbK →	FliA‑regulated DUF1537 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,791	0	T	C	100.0%	138.3 / NA	38	Y153H (TAT→CAT)	ygbK	FliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

ATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCT  >  NC_000913/2862645‑2862936                                                                                                                                                   |                                                                                                                                                  aTGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCt                                                                                                                                               >  2:744236/1‑151 (MQ=255)        cGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCg                                                                                                                                         <  2:648892/150‑1 (MQ=255)           cacGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTg                                                                                                                                     <  2:590739/151‑1 (MQ=255)                cGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGa                                                                                                                                <  2:369578/151‑1 (MQ=255)                cGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGa                                                                                                                                <  2:381611/151‑1 (MQ=255)                     aCGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAgc                                                                                                                             <  2:257140/149‑1 (MQ=255)                            tctctcCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATc                                                                                                                       <  2:209167/148‑1 (MQ=255)                                      ccTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGtgctgc                                                                                                          <  2:25577/151‑1 (MQ=255)                                            ggTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGcgtc                                                                                                    >  2:392789/1‑151 (MQ=255)                                                aaCGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGttt                                                                                                >  1:585627/1‑151 (MQ=255)                                                            gTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGttt                                                                                    <  2:452465/151‑1 (MQ=255)                                                                 tCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGa                                                                                >  1:168851/1‑150 (MQ=255)                                                                  cAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAAc                                                                              >  1:277803/1‑151 (MQ=255)                                                                    ggggTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACa                                                                             >  2:740500/1‑150 (MQ=255)                                                                       gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgt                                                                         <  2:78019/151‑1 (MQ=255)                                                                        tATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtg                                                                        >  2:530585/1‑151 (MQ=255)                                                                            tGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTgg                                                                     >  1:205667/1‑150 (MQ=255)                                                                               tCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGc                                                                 >  1:457853/1‑151 (MQ=255)                                                                               tCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGc                                                                 >  2:166928/1‑151 (MQ=255)                                                                                 gTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCg                                                               >  2:314345/1‑151 (MQ=255)                                                                                  tAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGt                                                              >  2:261037/1‑151 (MQ=255)                                                                                      gAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGACGCCGTTCGt                                                          >  2:688644/1‑151 (MQ=255)                                                                                       aaTCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                                                         <  1:87262/151‑1 (MQ=255)                                                                                           aaCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCa                                                        >  1:27114/1‑148 (MQ=255)                                                                                           aaCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCa                                                        >  1:672692/1‑148 (MQ=255)                                                                                                    ccGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCg                                            <  2:310885/151‑1 (MQ=255)                                                                                                          ccGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTAc                                       <  1:392740/150‑1 (MQ=255)                                                                                                           cGGGATGCGCCATCCCCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACa                                      <  1:83606/150‑1 (MQ=255)                                                                                                            gggATGCGCCATCACCTGGTAAATCCCATGACCGACAGCCATCTTCCACGTCTGGTTGAAGCGCAATCCACAGGCTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACa                                      >  2:169676/1‑149 (MQ=255)                                                                                                             ggATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCa                                   <  2:468968/151‑1 (MQ=255)                                                                                                             ggATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCa                                   <  2:700742/151‑1 (MQ=255)                                                                                                             ggATGCGCCATCACCCGGTAAATCCCAGGACCGACAGCCATCTTCCCCGTCGGGTTGAAGCGTAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTTTGGATGCCGGTCGTCAAAAGCTGGCTCGCTTACAGcg                                   <  1:609872/151‑2 (MQ=255)                                                                                                                      aTCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCt                           >  2:653610/1‑150 (MQ=255)                                                                                                                        cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                        <  1:740426/151‑1 (MQ=255)                                                                                                                               tAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTAcg                  >  2:469619/1‑150 (MQ=255)                                                                                                                                         gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGAt       >  1:558042/1‑151 (MQ=255)                                                                                                                                         gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGAt       <  2:722924/151‑1 (MQ=255)                                                                                                                                              aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt  <  2:681039/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCT  >  NC_000913/2862645‑2862936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑