Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,923,308	T→C	G159G (GGA→GGG)	truC ←	tRNA(Ile1,Asp) pseudouridine(65) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,923,308	0	T	C	100.0%	144.6 / NA	44	G159G (GGA→GGG)	truC	tRNA(Ile1,Asp) pseudouridine(65) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

CAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGTCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATC  >  NC_000913/2923161‑2923448                                                                                                                                                    |                                                                                                                                             cAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGccc                                                                                                                                            <  2:609479/150‑1 (MQ=255) cAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCg                                                                                                                                           >  1:220535/1‑151 (MQ=255)     gACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGtt                                                                                                                                        <  2:247565/150‑1 (MQ=255)      aCAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGtttt                                                                                                                                      <  1:537046/151‑1 (MQ=255)          ctgactgGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCgg                                                                                                                                   >  2:597442/1‑150 (MQ=255)          ctgactgGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGc                                                                                                                                  >  2:528507/1‑151 (MQ=255)             actgGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGc                                                                                                                                  <  1:271932/148‑1 (MQ=255)                  cATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTc                                                                                                                          >  2:704085/1‑151 (MQ=255)                                       ggCCAAAATGCTCAGCACCGCTGCGAATCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCACGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGCAGCGCGTg                                                                                                       >  1:28120/1‑149 (MQ=255)                                          cAAAATGCTCAGCAGCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCgg                                                                                                  <  1:648630/151‑1 (MQ=255)                                                gCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACg                                                                                            <  1:79937/151‑1 (MQ=255)                                                gCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAAGCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACg                                                                                            <  2:258448/151‑1 (MQ=255)                                                            tGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAc                                                                                >  2:361836/1‑151 (MQ=255)                                                             gCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAcc                                                                               <  2:356340/151‑1 (MQ=255)                                                             gCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAcc                                                                               >  1:390950/1‑151 (MQ=255)                                                             gCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAcc                                                                               <  2:332612/151‑1 (MQ=255)                                                              cGATTCTGGCGGAAATCGCCATGTTTGCTATCGCCAATAAACGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCTGGGTTTTCGGCTCCAGTTCCCCCAGGCCGTAGCGCGTGGTCGTGTAACGCCCGGTCGCTAcc                                                                               <  1:41900/150‑1 (MQ=255)                                                                aTTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGc                                                                            >  1:349773/1‑151 (MQ=255)                                                                           aaTCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGGAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGt                                                                 >  1:75320/1‑151 (MQ=255)                                                                             tCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCg                                                               >  2:612236/1‑151 (MQ=255)                                                                               gCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGcc                                                             >  1:667531/1‑151 (MQ=255)                                                                                ccATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCa                                                            <  2:555778/151‑1 (MQ=255)                                                                                        gCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACcgcg                                                     >  1:198490/1‑150 (MQ=255)                                                                                         cTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGAt                                                   >  2:313194/1‑151 (MQ=255)                                                                                          tATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGAt                                                   >  1:528991/1‑150 (MQ=255)                                                                                                cAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGt                                            <  2:26799/151‑1 (MQ=255)                                                                                                  ataatCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCa                                          <  2:487150/151‑1 (MQ=255)                                                                                                     atCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCActg                                       <  1:42352/151‑1 (MQ=255)                                                                                                       cGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCActgct                                     >  1:464214/1‑151 (MQ=255)                                                                                                             aCGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGc                               >  2:401457/1‑151 (MQ=255)                                                                                                              cGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCg                              <  2:293159/151‑1 (MQ=255)                                                                                                                 aaaTGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGc                           <  1:201240/151‑1 (MQ=255)                                                                                                                  aaTGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGcc                          >  1:462769/1‑151 (MQ=255)                                                                                                                    tGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCtt                        >  1:680141/1‑151 (MQ=255)                                                                                                                       gCAGGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTa                      <  1:55768/150‑1 (MQ=255)                                                                                                                           gATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTc                 <  1:117589/151‑1 (MQ=255)                                                                                                                                cGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCgg             <  2:146496/150‑1 (MQ=255)                                                                                                                                 ggCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGc           >  1:494624/1‑151 (MQ=255)                                                                                                                                 ggCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGc           >  2:649596/1‑151 (MQ=255)                                                                                                                                  gCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCa          <  2:127983/151‑1 (MQ=255)                                                                                                                                      aGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAAtt      >  2:648672/1‑151 (MQ=255)                                                                                                                                          gATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTa    <  1:381696/149‑1 (MQ=255)                                                                                                                                          gATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTAt   >  2:193179/1‑150 (MQ=255)                                                                                                                                          gATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATc  <  2:93327/151‑1 (MQ=255)                                                                                                                                           aTGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATc  >  1:537437/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                             CAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGTCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATC  >  NC_000913/2923161‑2923448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑