Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,942,711	A→G	intergenic (‑144/+207)	gcvA ← / ← ygdI	glycine cleavage system transcriptional activator; autorepressor/DUF903 family verified lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,942,711	0	A	G	100.0%	172.3 / NA	51	intergenic (‑144/+207)	gcvA/ygdI	glycine cleavage system transcriptional activator; autorepressor/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/22);  total (29/22)

GACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGT  >  NC_000913/2942563‑2942856                                                                                                                                                     |                                                                                                                                                  gACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCg                                                                                                                                                 >  1:187230/1‑151 (MQ=255)    aTGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGaa                                                                                                                                               >  2:24648/1‑150 (MQ=255)     tGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGaaa                                                                                                                                              >  2:425781/1‑150 (MQ=255)         tATTAAACTTTGATGTTAAATGACTTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGGGCCGGAGCGCAAAGttt                                                                                                                                         >  1:59909/1‑151 (MQ=255)             aaaCTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATc                                                                                                                                     >  2:290058/1‑151 (MQ=255)              aaCTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCg                                                                                                                                    >  2:381980/1‑151 (MQ=255)                  ttGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGa                                                                                                                                  <  1:459366/149‑1 (MQ=255)                    gATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGc                                                                                                                              <  2:707827/151‑1 (MQ=255)                     aTGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCg                                                                                                                             >  2:299959/1‑151 (MQ=255)                      tGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCgt                                                                                                                            >  2:97221/1‑151 (MQ=255)                       gTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCgt                                                                                                                            <  2:552417/150‑1 (MQ=255)                         tAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGtt                                                                                                                         >  1:385360/1‑151 (MQ=255)                           aaTGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCt                                                                                                                       <  1:627912/151‑1 (MQ=255)                            aTGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTg                                                                                                                      <  1:758243/151‑1 (MQ=255)                                 tttAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGa                                                                                                                  >  2:291794/1‑150 (MQ=255)                                        aTTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACtt                                                                                                              >  2:106657/1‑147 (MQ=255)                                        aTTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACtt                                                                                                              <  1:106625/147‑1 (MQ=255)                                          tAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGc                                                                                                         >  2:418120/1‑150 (MQ=255)                                                  acTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGtt                                                                                                 <  2:330250/150‑1 (MQ=255)                                                        tAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgt                                                                                          >  2:331289/1‑151 (MQ=255)                                                         aaCCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCTGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgtt                                                                                         >  1:482476/1‑151 (MQ=255)                                                          aCCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgttg                                                                                        >  2:467361/1‑151 (MQ=255)                                                               ttAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgttgtgtt                                                                                    >  2:234710/1‑150 (MQ=255)                                                                 aGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgttgtgttgtt                                                                                 <  2:759459/151‑1 (MQ=255)                                                                 aGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgttgtgttgtt                                                                                 >  2:415759/1‑151 (MQ=255)                                                                      ttttAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGATTTATCGGAAAGCGTGATCTGGTGAACTTTTGGTTTACGGTTGGGATgttgtgttgttgtgtt                                                                            >  1:446049/1‑151 (MQ=255)                                                                        ttAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGttt                                                                           <  1:57016/150‑1 (MQ=255)                                                                         tAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTg                                                                          <  1:527458/150‑1 (MQ=255)                                                                         tAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTg                                                                          <  2:453443/150‑1 (MQ=255)                                                                           aTCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCa                                                                        >  1:568041/1‑150 (MQ=255)                                                                             cTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGCTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAAtt                                                                     >  1:452975/1‑151 (MQ=255)                                                                                   cATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTg                                                               <  1:299926/151‑1 (MQ=255)                                                                                         aaaTTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGAtt                                                          <  1:104362/150‑1 (MQ=255)                                                                                            ttGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTc                                                         >  2:551664/1‑148 (MQ=255)                                                                                              gTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGAc                                                     <  1:274759/150‑1 (MQ=255)                                                                                                 cGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATg                                                 >  2:573143/1‑151 (MQ=255)                                                                                                    tGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGt                                               <  2:356229/150‑1 (MQ=255)                                                                                                    tGAGCGTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGGCTGCGATTCAGCCCATGGt                                               <  1:586130/150‑1 (MQ=255)                                                                                                                      tACCTATAGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTc                            >  2:422304/1‑151 (MQ=255)                                                                                                                            taGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTc                       <  2:623424/150‑1 (MQ=255)                                                                                                                             aGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCt                     <  1:422253/151‑1 (MQ=255)                                                                                                                             aGTGGCGGCACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCt                     <  2:21535/151‑1 (MQ=255)                                                                                                                             aGTGGCGGCACGTCCGGAGCCGAAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACGTTTGGGTTACGGTTGTGATGTTGTGTTGGTGTGTTTGGAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTGACTTTTTTCACGTcc                      <  1:264961/150‑1 (MQ=255)                                                                                                                                ggcggcACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGt                   >  2:461959/1‑150 (MQ=255)                                                                                                                                 gcggcACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTAc                 >  2:87134/1‑151 (MQ=255)                                                                                                                                   ggcACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACAt               >  2:364053/1‑151 (MQ=255)                                                                                                                                    gcACTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACAtt              <  1:405871/151‑1 (MQ=255)                                                                                                                                       cTTCCTGAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTAc           >  1:342419/1‑151 (MQ=255)                                                                                                                                             gAGCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCctgtc     <  1:319279/151‑1 (MQ=255)                                                                                                                                               gCCGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCctgtctg   >  2:448521/1‑151 (MQ=255)                                                                                                                                                ccGGAGCGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCctgtctgt  >  1:748317/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGT  >  NC_000913/2942563‑2942856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑