Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,986,699	T→C	L285L (TTG→CTG)	yqeG →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,986,699	0	T	C	100.0%	158.0 / NA	42	L285L (TTG→CTG)	yqeG	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/15);  total (27/15)

TCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCATTT  >  NC_000913/2986560‑2986839                                                                                                                                            |                                                                                                                                             tctcATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTcc                                                                                                                                   >  2:544716/1‑151 (MQ=255)    catcatCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCgg                                                                                                                                 <  1:568031/150‑1 (MQ=255)         tcTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGattat                                                                                                                           >  1:289354/1‑151 (MQ=255)         tcTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGattat                                                                                                                           >  1:657084/1‑151 (MQ=255)          cTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGattatt                                                                                                                          >  1:289836/1‑151 (MQ=255)           tGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTg                                                                                                                         >  2:116305/1‑151 (MQ=255)            gCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTg                                                                                                                         <  2:48264/150‑1 (MQ=255)             cATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCTCCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGattat                                                                                                                           <  1:561112/147‑1 (MQ=255)             cATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTc                                                                                                                       >  2:37457/1‑151 (MQ=255)                      aCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCagtagt                                                                                                               <  2:419465/150‑1 (MQ=255)                        tGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCa                                                                                                                    >  1:585716/1‑143 (MQ=255)                        tGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCa                                                                                                                    <  2:585773/143‑1 (MQ=255)                              ttGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTc                                                                                                      >  1:374521/1‑151 (MQ=255)                              ttGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTc                                                                                                      >  1:567122/1‑151 (MQ=255)                                  cTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGaa                                                                                                   >  2:450242/1‑150 (MQ=255)                                   tttAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAAt                                                                                                 >  2:310240/1‑151 (MQ=255)                                           cctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTg                                                                                         >  2:189588/1‑151 (MQ=255)                                                tttCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGgtac                                                                                    >  1:176241/1‑151 (MQ=255)                                                     aTTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGgtacgtac                                                                                <  2:290680/150‑1 (MQ=255)                                                       tCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACttt                                                                             >  1:723217/1‑151 (MQ=255)                                                       tCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACtt                                                                              <  1:45785/150‑1 (MQ=255)                                                           ccTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGc                                                                          <  2:164117/150‑1 (MQ=255)                                                                  atatTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGCTTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTg                                                                   >  2:179876/1‑150 (MQ=255)                                                                  atatTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTg                                                                   <  1:603517/150‑1 (MQ=255)                                                                     ttGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAgg                                                               >  1:220284/1‑151 (MQ=255)                                                                                aaaGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACagag                                                     <  1:544664/150‑1 (MQ=255)                                                                                    aagaagGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGgtcgt                                                >  2:473873/1‑151 (MQ=255)                                                                                             tCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAaacaac                                       >  1:459812/1‑151 (MQ=255)                                                                                               aCCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAacac                                     <  1:116273/151‑1 (MQ=255)                                                                                                  aTTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTAc                                  >  2:376699/1‑151 (MQ=255)                                                                                                       aTCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTAcagcag                             <  2:746332/151‑1 (MQ=255)                                                                                                           gcgcTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTg                         >  1:518145/1‑151 (MQ=255)                                                                                                            cgcTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTgg                        >  1:530264/1‑151 (MQ=255)                                                                                                                ttCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGgtg                      <  2:440144/149‑1 (MQ=255)                                                                                                                  cTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTaa                   >  1:504856/1‑150 (MQ=255)                                                                                                                    aTGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAg                 <  2:398310/150‑1 (MQ=255)                                                                                                                          ccGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTc          <  1:734945/151‑1 (MQ=255)                                                                                                                           cGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCg         >  2:187547/1‑151 (MQ=255)                                                                                                                           cGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCg         >  1:265327/1‑151 (MQ=255)                                                                                                                              aCGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGc      >  2:554123/1‑151 (MQ=255)                                                                                                                                 ccccAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCAtt   >  1:545630/1‑151 (MQ=255)                                                                                                                                   ccAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCAttt  >  2:607942/1‑150 (MQ=255)                                                                                                                                            |                                                                                                                                             TCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCATTT  >  NC_000913/2986560‑2986839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑