Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,000,899	A→G	E185E (GAA→GAG)	xdhA →	xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,000,899	0	A	G	100.0%	129.7 / NA	36	E185E (GAA→GAG)	xdhA	xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

CAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGG  >  NC_000913/3000751‑3001045                                                                                                                                                     |                                                                                                                                                   cAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGagag                                                                                                                                                  <  1:633772/151‑1 (MQ=255)  agaagaCGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGc                                                                                                                                                 <  1:251596/151‑1 (MQ=255)    aagaCGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGt                                                                                                                                               <  2:691358/151‑1 (MQ=255)       aCGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGCACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAAc                                                                                                                                            >  1:499792/1‑151 (MQ=255)                  aTCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGt                                                                                                                                 <  2:324141/151‑1 (MQ=255)                   tCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGt                                                                                                                                 >  2:220694/1‑150 (MQ=255)                    ccATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtg                                                                                                                                <  2:213502/150‑1 (MQ=255)                      aTAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtggat                                                                                                                             >  2:528661/1‑151 (MQ=255)                        aaCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtggatg                                                                                                                            <  2:488913/150‑1 (MQ=255)                         aCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtggatgg                                                                                                                           >  2:300763/1‑150 (MQ=255)                           ggTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATggagg                                                                                                                        >  1:244379/1‑151 (MQ=255)                                 aaTTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACATTCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACt                                                                                                                  <  1:298331/151‑1 (MQ=255)                                       cTGACACAAAGCACGATGTCGAAGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGCACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGa                                                                                                             >  1:45873/1‑150 (MQ=255)                                         gAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAAtt                                                                                                          >  1:236525/1‑151 (MQ=255)                                         gAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGCTATTCAACATTGTCACATGGCGAGCGTAACATCGCTGGCGTGGTTGGAGGATGACTCGCGAAtt                                                                                                          >  2:328098/1‑151 (MQ=255)                                           aaCAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTAc                                                                                                        <  1:401840/151‑1 (MQ=255)                                                 gCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGt                                                                                                  <  2:250827/151‑1 (MQ=255)                                                                    tGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCg                                                                               >  2:622156/1‑151 (MQ=255)                                                                    tGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCg                                                                               >  2:675479/1‑151 (MQ=255)                                                                       ccAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGcac                                                                            <  2:205634/151‑1 (MQ=255)                                                                               caaTCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTcg                                                                    <  2:60088/151‑1 (MQ=255)                                                                                   cGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAGCATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTcgccgc                                                                <  2:466933/151‑1 (MQ=255)                                                                                            cgACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGtggttggt                                                       >  2:592381/1‑151 (MQ=255)                                                                                                 aCCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGgc                                                  >  2:283727/1‑151 (MQ=255)                                                                                                     ggTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTg                                              <  2:763933/151‑1 (MQ=255)                                                                                                        aCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGat                                           >  1:37816/1‑151 (MQ=255)                                                                                                                   aTCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTc                                <  1:713392/151‑1 (MQ=255)                                                                                                                        aCCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCg                           <  2:236558/151‑1 (MQ=255)                                                                                                                              gTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAg                     <  1:541793/151‑1 (MQ=255)                                                                                                                                  ttCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTAc                        >  1:128655/1‑144 (MQ=255)                                                                                                                                  ttCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTAc                        <  2:128688/144‑1 (MQ=255)                                                                                                                                   tCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGtcatc                >  2:553465/1‑151 (MQ=255)                                                                                                                                       cATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAAc            <  2:441517/151‑1 (MQ=255)                                                                                                                                           gTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCAt         <  2:586352/150‑1 (MQ=255)                                                                                                                                               caTGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTc    <  2:418703/151‑1 (MQ=255)                                                                                                                                               caTGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTc    >  1:186673/1‑151 (MQ=255)                                                                                                                                                  ggAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCgg  <  1:322640/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGG  >  NC_000913/3000751‑3001045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑