Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	358,347	G→A	A115V (GCG→GTG)	cynR ←	transcriptional activator of cyn operon; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	358,347	0	G	A	100.0%	181.9 / NA	47	A115V (GCG→GTG)	cynR	transcriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/13);  total (34/13)

TCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCC  >  NC_000913/358203‑358482                                                                                                                                                 |                                                                                                                                        tCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACAt                                                                                                                                      >  1:143629/1‑148 (MQ=255)    gTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGaaa                                                                                                                                 <  1:320299/150‑1 (MQ=255)          aaaGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCa                                                                                                                          <  2:696766/151‑1 (MQ=255)            aGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCAtt                                                                                                                        <  2:626838/151‑1 (MQ=255)               aaTTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTa                                                                                                                       >  2:109531/1‑149 (MQ=255)                  tGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAgggg                                                                                                                  >  2:537760/1‑151 (MQ=255)                  tGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAgggg                                                                                                                  >  1:428304/1‑151 (MQ=255)                   gCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGc                                                                                                                 >  1:77214/1‑151 (MQ=255)                      tCCAGCTCCGGCGAGTGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCg                                                                                                               >  2:669702/1‑150 (MQ=255)                        cAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGAt                                                                                                             >  1:240506/1‑150 (MQ=255)                          gCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATaa                                                                                                           >  1:767528/1‑150 (MQ=255)                            tCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGt                                                                                                        >  1:245755/1‑151 (MQ=255)                            tCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGt                                                                                                        >  2:59667/1‑151 (MQ=255)                             ccGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGt                                                                                                        >  1:580295/1‑150 (MQ=255)                               ggCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGc                                                                                                     >  2:619568/1‑151 (MQ=255)                                  gAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCg                                                                                                  >  2:300506/1‑151 (MQ=255)                                      gCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTaa                                                                                               <  1:210654/150‑1 (MQ=255)                                         caGGGGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAATGCGTATCCTCGATTTTCTCCAGAGACATTTCCTGTGGCTGGAGCGTGATGCTGGGAGAGCGCACATAAAAATCCGCCATTAAGGGACCGATAAAGTAGCTCGTa                                                                                                <  1:266551/146‑1 (MQ=255)                                              gcgAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTggggg                                                                                      >  2:719826/1‑151 (MQ=255)                                                gAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTg                                                                                    >  2:197215/1‑151 (MQ=255)                                                 aaGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGGGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTg                                                                                    <  1:578347/150‑1 (MQ=255)                                                        tCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGAt                                                                            >  2:376835/1‑151 (MQ=255)                                                          ccAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATAc                                                                          >  2:159979/1‑151 (MQ=255)                                                           cAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACg                                                                         >  2:120870/1‑151 (MQ=255)                                                                    aCTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATcc                                                                >  1:320381/1‑151 (MQ=255)                                                                       cgtcgCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTcg                                                             <  1:551622/151‑1 (MQ=255)                                                                          cgcgGCAAAGCATATCCTCGATTTTCGCCTGTGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTcgcg                                                           <  2:214587/150‑1 (MQ=255)                                                                            cgGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTc                                                         >  2:159699/1‑150 (MQ=255)                                                                             ggCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTc                                                         >  1:190271/1‑149 (MQ=255)                                                                                aaaGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTc                                                         >  2:65185/1‑146 (MQ=255)                                                                                aaaGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATc                                                    <  2:204817/151‑1 (MQ=255)                                                                                 aaGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGCGGGGGTGACGGCGATACGCAGCGATCCTCGCGTc                                                         >  1:190608/1‑145 (MQ=255)                                                                                     atatCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCa                                                >  1:629652/1‑150 (MQ=255)                                                                                       atCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAca                                             <  2:669175/151‑1 (MQ=255)                                                                                       atCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAc                                              >  2:606793/1‑150 (MQ=255)                                                                                        tCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAcat                                            >  2:345105/1‑151 (MQ=255)                                                                                                    tcCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGccc                                >  2:361008/1‑151 (MQ=255)                                                                                                    tcCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGccc                                >  1:712644/1‑151 (MQ=255)                                                                                                     ccTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCg                               >  2:60324/1‑151 (MQ=255)                                                                                                       tGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGtt                             >  1:753686/1‑151 (MQ=255)                                                                                                               ttCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCgt                      <  1:482958/150‑2 (MQ=255)                                                                                                                  cTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGccccc                  >  2:665689/1‑151 (MQ=255)                                                                                                                    gTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAACCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCa                 <  1:502447/150‑1 (MQ=255)                                                                                                                            aGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGt         >  1:219664/1‑150 (MQ=255)                                                                                                                                tGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGc    >  2:549070/1‑151 (MQ=255)                                                                                                                                  aTGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGccc  >  1:470338/1‑151 (MQ=255)                                                                                                                                   tGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGccc  <  2:753761/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                        TCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCC  >  NC_000913/358203‑358482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑