Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,019,115	T→C	V1019A (GTC→GCC)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,019,115	0	T	C	100.0%	191.2 / NA	52	V1019A (GTC→GCC)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

CAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGTCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCG  >  NC_000913/3018969‑3019260                                                                                                                                                   |                                                                                                                                                  cagcagCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCccac                                                                                                                                               <  1:566047/151‑1 (MQ=255)                      tgtgGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATctgctg                                                                                                                          >  1:89578/1‑151 (MQ=255)                         ggAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGc                                                                                                                       >  2:651756/1‑151 (MQ=255)                         ggAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGc                                                                                                                       <  1:32544/151‑1 (MQ=255)                             gATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCcgcg                                                                                                                   <  1:614509/151‑1 (MQ=255)                                 gCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTgg                                                                                                                >  1:604942/1‑150 (MQ=255)                                  ccGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAg                                                                                                              <  2:185234/151‑1 (MQ=255)                                         cGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAAt                                                                                                       >  1:648557/1‑151 (MQ=255)                                                gTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTg                                                                                                >  1:230345/1‑151 (MQ=255)                                                   tGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGAtt                                                                                             >  2:735076/1‑151 (MQ=255)                                                          ccAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTga                                                                                         >  2:18679/1‑148 (MQ=255)                                                                 tGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCAc                                                                               >  2:392931/1‑151 (MQ=255)                                                                 tGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCAc                                                                               >  1:721818/1‑151 (MQ=255)                                                                  gggTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCa                                                                                <  2:26386/149‑1 (MQ=255)                                                                    gtgtTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGc                                                                            >  2:238591/1‑151 (MQ=255)                                                                    gtgtTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGc                                                                            <  2:206096/151‑1 (MQ=255)                                                                        tAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGt                                                                         <  1:7158/150‑1 (MQ=255)                                                                            cATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGc                                                                    >  1:283872/1‑151 (MQ=255)                                                                            cATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGc                                                                    >  1:227580/1‑151 (MQ=255)                                                                               cGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTac                                                                 <  2:230378/151‑1 (MQ=255)                                                                               cGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTac                                                                 >  2:240574/1‑151 (MQ=255)                                                                               cGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTac                                                                 >  1:441557/1‑151 (MQ=255)                                                                               cGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTa                                                                  <  2:384091/150‑1 (MQ=255)                                                                                gACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTaca                                                                <  2:210419/151‑1 (MQ=255)                                                                                    gCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCg                                                            <  2:158758/151‑1 (MQ=255)                                                                                    gCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCg                                                            >  1:395193/1‑151 (MQ=255)                                                                                     cAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCgg                                                           <  2:282897/151‑1 (MQ=255)                                                                                         ggTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCaa                                                        >  2:211504/1‑150 (MQ=255)                                                                                           tCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCaaa                                                       >  2:215795/1‑149 (MQ=255)                                                                                                ttAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGc                                                  >  2:343895/1‑149 (MQ=255)                                                                                                    caacGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGa                                              >  1:304154/1‑149 (MQ=255)                                                                                                    caacGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAg                                            <  1:155683/151‑1 (MQ=255)                                                                                                      acGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGc                                          <  2:107301/151‑1 (MQ=255)                                                                                                      acGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGc                                          <  1:211472/151‑1 (MQ=255)                                                                                                           ccaccGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTg                                      <  1:476967/150‑1 (MQ=255)                                                                                                                ggAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCg                                <  1:76042/151‑1 (MQ=255)                                                                                                                  aGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAGTGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGcc                              <  2:150782/151‑1 (MQ=255)                                                                                                                      gAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCg                          >  2:763588/1‑151 (MQ=255)                                                                                                                       aaCGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCg                          >  1:505904/1‑150 (MQ=255)                                                                                                                           atatGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGaaaa                      >  2:43192/1‑150 (MQ=255)                                                                                                                           atatGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAAc                     <  2:37139/151‑1 (MQ=255)                                                                                                                             atGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACg                    <  1:59598/150‑1 (MQ=255)                                                                                                                               gtgCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGaa                        >  2:317859/1‑144 (MQ=255)                                                                                                                               gtgCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGaa                        <  1:317826/144‑1 (MQ=255)                                                                                                                               gtgCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGAt                  <  2:515344/150‑1 (MQ=255)                                                                                                                                tgCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGAtgt                >  1:416257/1‑151 (MQ=255)                                                                                                                                    gCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGAtt            <  1:522859/151‑1 (MQ=255)                                                                                                                                      atcatcAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGt          >  2:720343/1‑151 (MQ=255)                                                                                                                                       tcatcaGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTc         >  1:111114/1‑151 (MQ=255)                                                                                                                                        catcaGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCg        <  2:695662/151‑1 (MQ=255)                                                                                                                                         atcaGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGc       <  2:389053/151‑1 (MQ=255)                                                                                                                                              ccATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCg  <  2:227613/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGTCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCG  >  NC_000913/3018969‑3019260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑