Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,038,017	G→A	Y30Y (TAC→TAT)	recJ ←	ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,038,017	0	G	A	100.0%	210.0 / NA	55	Y30Y (TAC→TAT)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/29);  total (26/29)

CACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCGTATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCTTTCATCTCTT  >  NC_000913/3037872‑3038164                                                                                                                                                  |                                                                                                                                                    cACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCAtata                                                                                                                                                 <  1:375974/150‑1 (MQ=255)     gACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACgg                                                                                                                                            >  2:533245/1‑151 (MQ=255)           aataatCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCgcagc                                                                                                                                      >  2:707572/1‑151 (MQ=255)             taatCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCgcagca                                                                                                                                     >  2:631439/1‑150 (MQ=255)              aatCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCgcagca                                                                                                                                     >  2:376540/1‑149 (MQ=255)                tCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAggg                                                                                                                                 >  2:496650/1‑151 (MQ=255)                 ccGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGa                                                                                                                                <  1:25295/151‑1 (MQ=255)                  cgcgTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAg                                                                                                                               <  2:730360/151‑1 (MQ=255)                   gcgTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAgg                                                                                                                              >  2:246722/1‑151 (MQ=255)                   gcgTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAgg                                                                                                                              >  1:55891/1‑151 (MQ=255)                    cgTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGc                                                                                                                             >  2:641203/1‑151 (MQ=255)                        ccTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAAtt                                                                                                                         >  2:234648/1‑151 (MQ=255)                           tCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTc                                                                                                                        <  1:669292/149‑1 (MQ=255)                           tCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTc                                                                                                                        <  2:253018/149‑1 (MQ=255)                                aaaaGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCGCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCgtg                                                                                                                  >  1:329986/1‑148 (MQ=255)                                   aGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCa                                                                                                                >  2:657722/1‑149 (MQ=255)                                      gTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCt                                                                                                           <  1:753152/151‑1 (MQ=255)                                              ggATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTc                                                                                                   >  1:661525/1‑151 (MQ=255)                                              ggATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTc                                                                                                   >  2:311870/1‑151 (MQ=255)                                                     aaCGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACt                                                                                            <  1:688255/151‑1 (MQ=255)                                                      aCGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACtt                                                                                           >  1:255737/1‑151 (MQ=255)                                                       cGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTc                                                                                          >  1:669323/1‑151 (MQ=255)                                                       cGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTc                                                                                          >  2:335138/1‑151 (MQ=255)                                                             tttCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACgg                                                                                      <  2:495768/149‑1 (MQ=255)                                                                cGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGcgac                                                                                  <  2:250658/150‑1 (MQ=255)                                                                    gccgcTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGt                                                                             <  1:171791/151‑1 (MQ=255)                                                                     ccgcTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGtt                                                                            >  2:109487/1‑151 (MQ=255)                                                                      cgcTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTg                                                                           <  2:83393/151‑1 (MQ=255)                                                                          cAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTa                                                                         <  1:391365/149‑1 (MQ=255)                                                                          cAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATc                                                                       <  2:202928/151‑1 (MQ=255)                                                                             ttGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCtgt                                                                    >  2:756496/1‑151 (MQ=255)                                                                               gctgcCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCtgtt                                                                   <  2:752471/150‑1 (MQ=255)                                                                                  gcCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGttt                                                               <  2:415403/151‑1 (MQ=255)                                                                                  gcCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGttt                                                               >  1:401732/1‑151 (MQ=255)                                                                                  gcCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGttt                                                               >  1:469924/1‑151 (MQ=255)                                                                                     aGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCAc                                                            >  2:708871/1‑151 (MQ=255)                                                                                       ggCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCAcgc                                                          >  2:711715/1‑151 (MQ=255)                                                                                         cagcaTACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCAcgcg                                                         >  1:70905/1‑150 (MQ=255)                                                                                            caTACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAatt                                                     <  2:340061/151‑1 (MQ=255)                                                                                               aCCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAattat                                                   <  1:311837/150‑1 (MQ=255)                                                                                               aCCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAattat                                                   <  1:707498/150‑1 (MQ=255)                                                                                                 cTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTAttt                                                 >  2:337431/1‑150 (MQ=255)                                                                                                                ccAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCAttttt                                 <  1:63904/151‑1 (MQ=255)                                                                                                                ccAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCAttttt                                 <  1:404979/151‑1 (MQ=255)                                                                                                                    ttCTTGCGCACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGgt                             >  1:556866/1‑151 (MQ=255)                                                                                                                          cgcACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTtcgt                       <  1:80279/151‑1 (MQ=255)                                                                                                                          cgcACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTtcgt                       <  1:83271/151‑1 (MQ=255)                                                                                                                           gcACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTtcgtc                      <  2:320411/151‑1 (MQ=255)                                                                                                                           gcACTGCGTACTCCCCGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTtcgtc                      <  1:38003/151‑1 (MQ=255)                                                                                                                                       ccccGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCttt          <  1:713564/151‑1 (MQ=255)                                                                                                                                          cGGCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCTTTCAt       <  2:458799/151‑1 (MQ=255)                                                                                                                                            gCTGGCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCTTTCAtct     <  2:205664/151‑1 (MQ=255)                                                                                                                                               ggCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCTTTCAtctc    <  2:669381/149‑1 (MQ=255)                                                                                                                                               ggCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCTTTCATCTCtt  <  1:46726/151‑1 (MQ=255)                                                                                                                                               ggCATATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCTTTCATCTCtt  >  2:229735/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCACTGCGTACTCCCCGGCTGGCGTATAAACGGCGCAGCAAGGGAGGCAATTCAGCGGGCAAGTCTGCCGTTTCATCGACTTCACGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATTATTTACCGCTGGTCATTTTTTGGTGTTCGTCGAGGAATTCTTTCATCTCTT  >  NC_000913/3037872‑3038164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑