Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,083,334	T→C	Y155C (TAT→TGT)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,083,334	0	T	C	98.0%	181.6 / ‑4.3	51	Y155C (TAT→TGT)	speB	agmatinase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (20/30);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGC  >  NC_000913/3083189‑3083479                                                                                                                                                  |                                                                                                                                                  cTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAgg                                                                                                                                                >  1:499620/1‑149 (MQ=255) cTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGt                                                                                                                                               >  1:497448/1‑150 (MQ=255)        gCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGgtgt                                                                                                                                       <  2:131433/151‑1 (MQ=255)            ccAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTggg                                                                                                                                    <  2:529328/150‑1 (MQ=255)                   ggTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCaa                                                                                                                              >  2:30715/1‑149 (MQ=255)                   ggTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAg                                                                                                                            <  1:35796/151‑1 (MQ=255)                       aaGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTa                                                                                                                        <  1:759493/151‑1 (MQ=255)                           cGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTAcc                                                                                                                      >  1:263959/1‑149 (MQ=255)                            gTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGCCCTTCTTTCGGCGCGTGATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTAGGCGTCAAAGTGTACCAgc                                                                                                                   <  1:512706/151‑1 (MQ=255)                                tCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGcc                                                                                                                >  2:702010/1‑150 (MQ=255)                                   ttATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCa                                                                                                               <  2:555095/148‑1 (MQ=255)                                    tATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCAttt                                                                                                            <  2:263992/150‑1 (MQ=255)                                     aTCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCAtttt                                                                                                           <  1:535904/150‑1 (MQ=255)                                       cAAACTAGGAACGAATACCAATTTGCGCGGCATGATTCGGGTCGATCAGACATTCTTTCGGCGCGGTATAGAACACAGTGCCGTGGGCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGcc                                                                                                        >  1:324256/1‑151 (MQ=255)                                              ggTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGc                                                                                                 >  1:230179/1‑151 (MQ=255)                                               gTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGc                                                                                                 <  1:718823/150‑1 (MQ=255)                                                 aCGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCtt                                                                                               <  1:229882/150‑1 (MQ=255)                                                  cGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCg                                                                                             <  1:114744/151‑1 (MQ=255)                                                      tACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATg                                                                                         >  1:649524/1‑151 (MQ=255)                                                         cAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGc                                                                                      <  2:725764/151‑1 (MQ=255)                                                         cAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGc                                                                                      >  2:293818/1‑151 (MQ=255)                                                           aTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCAc                                                                                    >  2:304665/1‑151 (MQ=255)                                                           aTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACAGAGTGCCGTGGTCAAATTCACAACCGTTCTCACAGGTATCGGTGTGGGCGTCTAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATAAGCa                                                                                     >  1:273713/1‑150 (MQ=255)                                                               gCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCAc                                                                                    <  2:601889/147‑1 (MQ=255)                                                                 aCGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACgcagca                                                                              <  1:429040/151‑1 (MQ=255)                                                                 aCGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACgcagc                                                                               >  1:333730/1‑150 (MQ=255)                                                                   ggAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCagcagc                                                                            <  2:468091/151‑1 (MQ=255)                                                                   ggAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCagcagc                                                                            <  2:39020/151‑1 (MQ=255)                                                                    gAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcg                                                                           >  2:249124/1‑151 (MQ=255)                                                                      aTGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggc                                                                         <  2:62434/151‑1 (MQ=255)                                                                         aTTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag                                                                       <  2:264088/150‑1 (MQ=255)                                                                             gggTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTa                                                                   >  2:207682/1‑150 (MQ=255)                                                                                                tCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGa                                                <  1:637559/150‑1 (MQ=255)                                                                                                 cGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGaa                                               <  1:94601/150‑1 (MQ=255)                                                                                                      cgGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAagagag                                         <  1:261118/151‑1 (MQ=255)                                                                                                            aGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACg                                   >  1:451840/1‑151 (MQ=255)                                                                                                                  tAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTAcc                             >  2:11170/1‑151 (MQ=255)                                                                                                                        cGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGcagc                       <  1:376261/151‑1 (MQ=255)                                                                                                                             tCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAaga                                            >  2:577906/1‑125 (MQ=255)                                                                                                                             tCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAaga                                            <  1:577849/125‑1 (MQ=255)                                                                                                                              cAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGcagccag                    <  2:684175/148‑1 (MQ=255)                                                                                                                              cAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCcagca                  <  2:767775/150‑1 (MQ=255)                                                                                                                                 aTTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCtt              >  1:740125/1‑151 (MQ=255)                                                                                                                                     acaACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCg          >  2:75557/1‑151 (MQ=255)                                                                                                                                      caACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCgg         <  2:333763/151‑1 (MQ=255)                                                                                                                                       aaCCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGc        <  1:701936/151‑1 (MQ=255)                                                                                                                                        aCCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCg       <  2:230212/151‑1 (MQ=255)                                                                                                                                          cGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGt      >  1:230559/1‑150 (MQ=255)                                                                                                                                           gTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTgc    >  2:288417/1‑151 (MQ=255)                                                                                                                                           gTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTgc    <  1:676123/151‑1 (MQ=255)                                                                                                                                              cGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTgcgc  <  1:264451/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGC  >  NC_000913/3083189‑3083479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑