Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,108,362	G→A	A265V (GCG→GTG)	speC ←	ornithine decarboxylase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,108,362	0	G	A	100.0%	141.1 / NA	39	A265V (GCG→GTG)	speC	ornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/17);  total (22/17)

CGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGAC  >  NC_000913/3108216‑3108505                                                                                                                                                   |                                                                                                                                                cGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAAc                                                                                                                                             >  2:515196/1‑151 (MQ=255)   aCCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGt                                                                                                                                           <  2:743536/151‑1 (MQ=255)    cccACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTc                                                                                                                                          <  1:185719/151‑1 (MQ=255)          cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAt                                                                                                                                    >  1:349115/1‑151 (MQ=255)          cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAt                                                                                                                                    <  1:408950/151‑1 (MQ=255)                 aaaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTgg                                                                                                                             >  2:761460/1‑151 (MQ=255)                     aGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGTGAATTTGCTGGCGCa                                                                                                                         <  2:11523/151‑1 (MQ=255)                       aaTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgcgc                                                                                                                          >  1:241947/1‑148 (MQ=255)                        aTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGAt                                                                                                                      <  1:370059/151‑1 (MQ=255)                        aTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTAAAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGAt                                                                                                                      <  2:319878/151‑1 (MQ=255)                          gTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATa                                                                                                                     <  1:593906/150‑1 (MQ=255)                            aaTCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATActc                                                                                                                  >  2:236197/1‑151 (MQ=255)                                  acaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                                                                                            >  2:506530/1‑151 (MQ=255)                                   caGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                                                                                            >  1:118040/1‑150 (MQ=255)                                     gATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaaa                                                                                                         <  1:699262/151‑1 (MQ=255)                                     gATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaa                                                                                                          >  1:247934/1‑150 (MQ=255)                                        gCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAAc                                                                                                       >  2:105133/1‑150 (MQ=255)                                        gCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAAc                                                                                                       >  2:615685/1‑150 (MQ=255)                                         cccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAg                                                                                                     <  2:274237/151‑1 (MQ=255)                                                    cGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAAt                                                                                          <  1:238135/151‑1 (MQ=255)                                                    cGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAAt                                                                                          <  2:513445/151‑1 (MQ=255)                                                      aTCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATAc                                                                                        <  2:359646/151‑1 (MQ=255)                                                             gACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCa                                                                                   <  2:125836/149‑1 (MQ=255)                                                                  gCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGaaa                                                                             >  1:262638/1‑150 (MQ=255)                                                                     ttATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCa                                                                          >  2:520903/1‑150 (MQ=255)                                                                      tataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCaa                                                                         <  2:719650/150‑1 (MQ=255)                                                                       ataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAAc                                                                       >  1:438235/1‑151 (MQ=255)                                                                                gCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGt                                                              >  2:286671/1‑151 (MQ=255)                                                                                             cccAGCTGAATAACCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATCCTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt                                                 <  2:384467/151‑1 (MQ=255)                                                                                                        aatCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACACCGTCGCGAATTTGCTGGCGCAGATACTTTTCTTTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGt                                       <  2:579623/150‑1 (MQ=255)                                                                                                         atCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCg                                     >  2:765351/1‑151 (MQ=255)                                                                                                               aGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGccccc                                >  2:640711/1‑150 (MQ=255)                                                                                                                  cGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGcc                             >  1:776024/1‑150 (MQ=255)                                                                                                                  cGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCt                            >  2:66909/1‑151 (MQ=255)                                                                                                                     tACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGa                          >  2:545881/1‑150 (MQ=255)                                                                                                                         ggCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCGTCCAGATAGACCGGCGTCGCCCCCGCCTGAAt                        >  1:27350/1‑148 (MQ=255)                                                                                                                                          ttttCTGGCACAACGTCGCGAATTTGCGGGCGCAGATACTCTTCATAAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGAtt      <  2:454850/149‑1 (MQ=255)                                                                                                                                           tttCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCg    >  2:352961/1‑150 (MQ=255)                                                                                                                                            ttCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGAc  >  2:89107/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                CGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGAC  >  NC_000913/3108216‑3108505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑