Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,118,599 C→T V172I (GTT→ATT)  sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,118,5990CT100.0% 206.8 / NA 57V172I (GTT→ATT) sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/25);  total (32/25)

CAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGT  >  NC_000913/3118459‑3118743
                                                                                                                                            |                                                                                                                                                
cAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtt                                                                                                                                        >  2:756775/1‑151 (MQ=255)
  gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtttt                                                                                                                                      >  1:567458/1‑151 (MQ=255)
     ttttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCt                                                                                                                                    <  1:713139/150‑1 (MQ=255)
         gAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCa                                                                                                                               <  1:743489/151‑1 (MQ=255)
              ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCg                                                                                                                            <  2:681864/149‑1 (MQ=255)
                 ccGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGc                                                                                                                        <  2:131005/150‑1 (MQ=255)
                   ggTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCg                                                                                                                     <  1:702109/151‑1 (MQ=255)
                       ccAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGcc                                                                                                                   >  2:34740/1‑149 (MQ=255)
                         aGATCGATTTGCTTATACAGCGAGTCGAAGCTTTTACTCTCGATCACCGAGGAGAACGTCAGACAACCCTGTTCTGTATTCGCCGGACAGCTGTTCCTGGCCGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt                                                                                                                <  1:58288/150‑1 (MQ=255)
                          gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                              >  2:467684/1‑151 (MQ=255)
                          gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                              >  1:61175/1‑151 (MQ=255)
                              gATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgc                                                                                                          >  1:525310/1‑151 (MQ=255)
                               aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                                                                         >  2:638787/1‑151 (MQ=255)
                                 ttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                                                                        <  2:770204/150‑1 (MQ=255)
                                  agCTTATACAGCGAGTCCAAGCGTTTACTCTCGATCACCGAGGAGCACGTCAGGCAAACCTGCTCAGTATTCGCCGGACAGCTGTTACTGGCCGGCACCAGCGAAATCGCATTGCTTTTCTTGTCGTCGCATCCTGCCACGTCCTGCGCGt                                                                                                      >  1:16934/2‑151 (MQ=255)
                                       ataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTc                                                                                                  <  1:414925/150‑1 (MQ=255)
                                        taCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCa                                                                                                 >  1:353028/1‑150 (MQ=255)
                                            gCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCt                                                                                            >  2:490622/1‑151 (MQ=255)
                                               aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTaa                                                                                          >  2:644541/1‑150 (MQ=255)
                                                     aGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGacac                                                                                   >  1:250658/1‑151 (MQ=255)
                                                               ctcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCaa                                                                           <  1:605675/149‑1 (MQ=255)
                                                                 cGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgc                                                                       >  2:67517/1‑151 (MQ=255)
                                                                 cGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcg                                                                        <  1:129468/150‑1 (MQ=255)
                                                                   aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcac                                                                     >  2:404429/1‑151 (MQ=255)
                                                                    tccacaagtggaACGTCACCCAACCCTGTTCTGTATTCGCCGGACAGCTGTTACGGGACGTTACCAGCGAAATCACATTGCTTTTCTAGCCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAGCcgca                                                                      <  1:300121/139‑1 (MQ=255)
                                                                     cACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcacgc                                                                   >  1:225147/1‑151 (MQ=255)
                                                                     cACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcacgc                                                                   <  1:764239/151‑1 (MQ=255)
                                                                       ccGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcacgca                                                                  >  2:345708/1‑150 (MQ=255)
                                                                       ccGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCaa                                                                 <  1:149813/151‑1 (MQ=255)
                                                                         gaggagAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGc                                                               >  1:237059/1‑151 (MQ=255)
                                                                          aggagAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCt                                                              >  1:261558/1‑151 (MQ=255)
                                                                           ggagAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTa                                                             <  1:246146/151‑1 (MQ=255)
                                                                            gagaGCGGCAGACAAACCGGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTCCCAGCGAAATCGCATTGCTTTGCTTGTTATCGGAGCCCGCCAGGTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTa                                                             <  1:653721/150‑1 (MQ=255)
                                                                                  gTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACgcgc                                                        >  1:199094/1‑149 (MQ=255)
                                                                                  gTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAg                                                      <  2:82675/151‑1 (MQ=255)
                                                                                       aCAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTct                                                 >  2:662720/1‑151 (MQ=255)
                                                                                          aaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTc                                                                             <  2:157419/120‑1 (MQ=255)
                                                                                          aaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTc                                                                             >  1:157386/1‑120 (MQ=255)
                                                                                           aCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgact                                             >  1:350877/1‑151 (MQ=255)
                                                                                             cTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgactg                                            >  2:378002/1‑150 (MQ=255)
                                                                                               gTTCTGTGTTCGCCGGACAGCTGTTCCTGGACGTTCCCAGCGAAATCGCATTGCTTTTCTTGTCATCGGCGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAg                                          <  1:459118/150‑1 (MQ=255)
                                                                                               gTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgt                                         <  2:643430/151‑1 (MQ=255)
                                                                                                ttCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgt                                         <  1:118297/150‑1 (MQ=255)
                                                                                                   tGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTg                                     >  2:501598/1‑151 (MQ=255)
                                                                                                        tCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAgg                                 <  2:64492/150‑1 (MQ=255)
                                                                                                           ccGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGc                             <  1:396667/151‑1 (MQ=255)
                                                                                                             ggACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCa                            <  2:525362/150‑1 (MQ=255)
                                                                                                             ggACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCa                            >  2:99205/1‑150 (MQ=255)
                                                                                                                   cTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTc                      <  1:250894/150‑1 (MQ=255)
                                                                                                                        aCTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGtt                 >  2:23129/1‑150 (MQ=255)
                                                                                                                         cTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGCGGCAATTGTCGTGTTAc               >  1:230383/1‑151 (MQ=255)
                                                                                                                           ggACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTAccc             >  2:304163/1‑151 (MQ=255)
                                                                                                                                  aCCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGc      >  1:441551/1‑151 (MQ=255)
                                                                                                                                    cAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCa     >  1:112491/1‑150 (MQ=255)
                                                                                                                                      gCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCa     <  2:107973/148‑1 (MQ=255)
                                                                                                                                       cGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt  >  2:25633/1‑150 (MQ=255)
                                                                                                                                       cGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt  >  1:31053/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGT  >  NC_000913/3118459‑3118743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: