Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	359,143	T→C	H115H (CAT→CAC)	cynT →	carbonic anhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	359,143	0	T	C	100.0%	184.1 / NA	50	H115H (CAT→CAC)	cynT	carbonic anhydrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/21);  total (29/21)

CCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAACTCAT  >  NC_000913/359000‑359287                                                                                                                                                |                                                                                                                                                 ccTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGGTGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgcctg                                                                                                                                           <  1:241021/151‑1 (MQ=255) ccTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgcctg                                                                                                                                           >  2:246241/1‑151 (MQ=255)     ccTCCGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCg                                                                                                                                        <  1:602347/150‑1 (MQ=255)     ccTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGt                                                                                                                                       <  2:393070/151‑1 (MQ=255)          gggCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTccc                                                                                                                                  >  1:395600/1‑151 (MQ=255)             ccGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCa                                                                                                                                 >  1:29201/1‑149 (MQ=255)             ccGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACt                                                                                                                               >  2:88778/1‑151 (MQ=255)                 aaCCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCt                                                                                                                           >  2:107480/1‑151 (MQ=255)                   cccGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTg                                                                                                                          <  1:600029/150‑1 (MQ=255)                     cGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGt                                                                                                                       >  1:587881/1‑151 (MQ=255)                         ggCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTa                                                                                                                     <  1:511135/149‑1 (MQ=255)                         ggCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATg                                                                                                                   >  1:247738/1‑151 (MQ=255)                               tCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGAt                                                                                                              <  2:510715/150‑1 (MQ=255)                                 tGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTc                                                                                                            >  2:349683/1‑150 (MQ=255)                                       ggTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCcgc                                                                                                     >  1:515226/1‑151 (MQ=255)                                          gcggTATGAGGTGGCTGCGTTCCGGTGTTCTGACATTGTGATCTGTGGTCAGTCCAACGGTGGCGGGATGACCGCCATTGCCAGCTTTGAGTGCATGGCCCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGGTTCAGCCCGcgtt                                                                                                  <  1:653326/148‑2 (MQ=255)                                           gAGTTTGCCGTCGCTGCGCTGCGGATATTTGACATGGTGTTTGGTGGTTAATCCAGCTGGGGCGCGAGGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGcgtcg                                                                                                 <  1:474538/151‑1 (MQ=255)                                            aGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGcgtcg                                                                                                 >  1:465258/1‑150 (MQ=255)                                             gTATGCCGTCGCTGCGCTTCGGTTATCTGACATTGTGATTTGTGATCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACGGGCTGCGTTATGCCGATTCAGCCCGcgtcg                                                                                                 <  1:192674/149‑1 (MQ=255)                                               aTGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTaa                                                                                             <  1:183392/151‑1 (MQ=255)                                                  ccGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATg                                                                                           >  1:509576/1‑150 (MQ=255)                                                         tGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGcc                                                                                                                 >  2:489271/1‑121 (MQ=255)                                                           cgcTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGcc                                                                                                                 <  1:489219/119‑1 (MQ=255)                                                                cGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCAtt                                                                            >  2:534663/1‑151 (MQ=255)                                                                   gTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCg                                                                         >  2:2149/1‑151 (MQ=255)                                                                      tCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGAtt                                                                      >  1:93278/1‑151 (MQ=255)                                                                        tGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGAttt                                                                     >  2:72113/1‑150 (MQ=255)                                                                                tgATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCaa                                                             >  1:438819/1‑150 (MQ=255)                                                                                       tgGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTgcg                                                     >  2:442128/1‑151 (MQ=255)                                                                                          tCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTgcggcg                                                  >  2:756879/1‑151 (MQ=255)                                                                                             ttCCAACTGTGGCGCTAGGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGAt                                                <  2:622457/150‑1 (MQ=255)                                                                                              tCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATgg                                              >  1:721226/1‑151 (MQ=255)                                                                                               ccAACTGTGGCGCGATGGCCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATg                                               <  2:485755/149‑1 (MQ=255)                                                                                                cAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTa                                            <  1:286325/151‑1 (MQ=255)                                                                                                    tgtgGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTg                                        >  1:182339/1‑151 (MQ=255)                                                                                                      tgGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGa                                       >  2:481031/1‑150 (MQ=255)                                                                                                                  cGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCt                          >  1:228763/1‑151 (MQ=255)                                                                                                                    ccATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTc                         >  1:679359/1‑150 (MQ=255)                                                                                                                      aTTGCCCGCTGTCAGTGCATGGACCACATGACTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTATCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATGGCTCAGt                      >  1:605583/1‑151 (MQ=255)                                                                                                                      aTTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGAt                                                <  2:461183/125‑1 (MQ=255)                                                                                                                      aTTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGAt                                                >  1:461132/1‑125 (MQ=255)                                                                                                                      aTTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGt                      <  1:92817/151‑1 (MQ=255)                                                                                                                        tGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTg                    >  2:116372/1‑151 (MQ=255)                                                                                                                         gCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTg                    <  2:142444/150‑1 (MQ=255)                                                                                                                         gCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTg                    <  1:298396/150‑1 (MQ=255)                                                                                                                                  cAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGc          <  1:639437/151‑1 (MQ=255)                                                                                                                                    gTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCa         >  2:243238/1‑150 (MQ=255)                                                                                                                                      gCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAc      >  1:613430/1‑151 (MQ=255)                                                                                                                                          ggACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAACTca   <  2:109996/150‑1 (MQ=255)                                                                                                                                            aCCACATGCCTGCCGTCTCCCACTGCCTGCGTTATGCCGATTCAGCCCGCGCCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTAAAAAGCTTCGGCGATGGTACGTGAAAACGTCATTGCTCATTTGGCTAATTTGCAAACTcat  <  1:548459/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                 CCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAACTCAT  >  NC_000913/359000‑359287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑