Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,231,746	A→G	T28A (ACC→GCC)	fadH →	2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,231,746	0	A	G	100.0%	127.8 / NA	37	T28A (ACC→GCC)	fadH	2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

CGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCG  >  NC_000913/3231601‑3231881                                                                                                                                                  |                                                                                                                                        cGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCggg                                                                                                                                    <  2:25823/151‑1 (MQ=255) cGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCggg                                                                                                                                    <  1:688868/151‑1 (MQ=255)     gACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTgg                                                                                                                                >  2:765339/1‑151 (MQ=255)        ggCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTggagg                                                                                                                             >  2:206825/1‑151 (MQ=255)            aaaTTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAAt                                                                                                                          >  2:11681/1‑150 (MQ=255)            aaaTTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAAt                                                                                                                          <  1:83422/150‑1 (MQ=255)              aTTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATAcc                                                                                                                       <  2:737402/151‑1 (MQ=255)                  gAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGa                                                                                                                   <  1:490942/151‑1 (MQ=255)                         tCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGcc                                                                                                            >  2:456695/1‑151 (MQ=255)                            gACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCg                                                                                                           >  2:241491/1‑149 (MQ=255)                            gACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAg                                                                                                         <  1:408936/151‑1 (MQ=255)                            gACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAg                                                                                                         <  2:480122/151‑1 (MQ=255)                                         ttattTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGt                                                                                            >  1:77188/1‑151 (MQ=255)                                          tattTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGtt                                                                                           >  1:132985/1‑151 (MQ=255)                                           attTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGtt                                                                                           <  1:635334/150‑1 (MQ=255)                                                       ggACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAAcgc                                                                              >  1:713518/1‑151 (MQ=255)                                                        gACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAAcgc                                                                              >  1:645451/1‑150 (MQ=255)                                                            aTTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCg                                                                         >  1:233059/1‑151 (MQ=255)                                                                     gCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGt                                                                <  1:241044/151‑1 (MQ=255)                                                                         cccGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGgcg                                                            >  2:448760/1‑151 (MQ=255)                                                                           cGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCt                                                          <  1:694592/151‑1 (MQ=255)                                                                             tCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGgcgc                                                           >  1:41112/1‑148 (MQ=255)                                                                             tCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTg                                                         >  2:607263/1‑150 (MQ=255)                                                                               gCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                      <  2:395570/151‑1 (MQ=255)                                                                               gCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                      <  1:411793/151‑1 (MQ=255)                                                                               gCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                      >  2:441592/1‑151 (MQ=255)                                                                                  gTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTg                                                     <  2:324954/149‑1 (MQ=255)                                                                                      gCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAgcg                                               >  1:527552/1‑151 (MQ=255)                                                                                             tGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCAt                                        >  1:712413/1‑151 (MQ=255)                                                                                               gATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACTGCGTGGCGCTGATTGTCAGCGGCGGCATTg                                      <  1:688377/151‑1 (MQ=255)                                                                                                          tACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGAttt                            >  2:2104/1‑150 (MQ=255)                                                                                                           aCCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTa                           <  2:592236/150‑1 (MQ=255)                                                                                                            ccACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAAc                         <  2:342241/151‑1 (MQ=255)                                                                                                                      aaaCCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGc               >  1:487340/1‑151 (MQ=255)                                                                                                                       aaCCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCa              <  1:561596/151‑1 (MQ=255)                                                                                                                                 tGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTg     <  2:406030/150‑1 (MQ=255)                                                                                                                                   aTGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGgcg  <  1:590269/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                        CGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCG  >  NC_000913/3231601‑3231881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑