Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,428	T→C	E438E (GAA→GAG)	uxaC ←	uronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,428	0	T	C	100.0%	163.4 / NA	46	E438E (GAA→GAG)	uxaC	uronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/19);  total (27/19)

ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTT  >  NC_000913/3243280‑3243570                                                                                                                                                     |                                                                                                                                               aCGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTc                                                                                                                                              >  1:155705/1‑151 (MQ=255)                  gATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGatc                                                                                                                              >  1:421537/1‑150 (MQ=255)                        gATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGc                                                                                                                       >  2:312294/1‑151 (MQ=255)                         atttATTGCATATCACCCCCAGACATTAGTTCAGTTAAGCGGAGAAGTAGTCACGCGCATGGTTAAAGAAAATATTTTCCACCACCTCCCCGAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGc                                                                                                                       <  1:756416/147‑1 (MQ=255)                                gCATATCACCCCCAGACCTTAGTTCAGTTCAAGGGCGAAGTAGTCACGCGCATCGTTAAAGCAAATATTTTTCCCCACCCCGCCCAGCGGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAAt                                                                                                                <  1:664673/150‑1 (MQ=255)                                  atatCAACCCCAGCCCTTAGTTCAGTTCAATGGCGAAGTAGTCCCGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgc                                                                                                              <  2:200776/150‑1 (MQ=255)                                   tatCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcgg                                                                                                            >  2:516900/1‑151 (MQ=255)                                      cAACCCCAGACCTTATTGCAGTTAAATGGAGAAGTAGTCCCGCGCATTTTTAAAGCAAATTTTTTCCCCCACCTCGCCACGCAGGTTGATGTCCCCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggc                                                                                                           <  1:505575/149‑1 (MQ=255)                                      cAACCCCAGACCTTAGTTCAGTTCAAGGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggcg                                                                                                          <  2:59055/150‑1 (MQ=255)                                         ccccATACTTTAGTTCAGTTCAATGGCCAATGGGCCGCGCGCATTTTTAAAGCAAATATTTGTCCCCATCCCGCCCGGGAGGTTGATGCCCGCCGGGCGTGGGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGaa                                                                                                       <  1:555256/150‑1 (MQ=255)                                              gACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCCCGCCCAGCAGGTTGATGTCCGCGGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTAtt                                                                                                  <  2:420409/150‑1 (MQ=255)                                                ccTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGt                                                                                               >  2:738392/1‑151 (MQ=255)                                                 cTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGt                                                                                               >  2:613679/1‑150 (MQ=255)                                                   tAGTTCATTTCAAGGGCGAAGTAGTCCCGCGCATTTTTAAAGCAAATATTTTCCACCACCCCGCCCAGCAGGTTGATGCCCGCCGGTGCTGCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTg                                                                                              <  1:19892/149‑1 (MQ=255)                                                   tAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGa                                                                                             >  2:389859/1‑150 (MQ=255)                                                    agttcagttaAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACg                                                                                           <  2:30980/151‑1 (MQ=255)                                                          gttcaATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGgtgt                                                                                      >  1:262596/1‑150 (MQ=255)                                                                 ggCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCGGCAGGTTGATGTCCCCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAgg                                                                              <  2:757419/151‑1 (MQ=255)                                                                     aaGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGc                                                                          >  2:28925/1‑151 (MQ=255)                                                                         agtCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTa                                                                                                    >  2:458023/1‑121 (MQ=255)                                                                                     ttttAAACAAAATATTTTTCCCCATCTCCCCCAGCAGTTTGATGTCCGCCGGTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtcagtca                                                           <  1:553620/148‑1 (MQ=255)                                                                                      gTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCCGTCAGc                                                         >  1:737031/1‑151 (MQ=255)                                                                                      gTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGc                                                         >  2:420389/1‑151 (MQ=255)                                                                                         aaaGCAAATATTTTTCCCCATCTCGCCCAGCGGGTTGATGTCCGCGGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATa                                                      <  2:2225/151‑1 (MQ=255)                                                                                         aaaGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                       >  2:42586/1‑150 (MQ=255)                                                                                          aaGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATAc                                                     >  1:618308/1‑151 (MQ=255)                                                                                           agaaaaTCTTTTCCCCCATCCCGCCCAGCGGGTTGAGCCCCGCGGGTGCTTCCCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATa                                                      <  1:160596/146‑1 (MQ=255)                                                                                            gCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCg                                                   >  2:567545/1‑151 (MQ=255)                                                                                              aaaTATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                 <  2:639023/151‑1 (MQ=255)                                                                                               aaTATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                 >  2:17524/1‑150 (MQ=255)                                                                                                     tttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCgg                                          >  2:71507/1‑151 (MQ=255)                                                                                                     tttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCgg                                          >  1:416441/1‑151 (MQ=255)                                                                                                         aCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTc                                       >  2:450194/1‑150 (MQ=255)                                                                                                          ccATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAAAAAGCGGCTc                                       >  2:605014/1‑149 (MQ=255)                                                                                                           cATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                    >  2:294105/1‑151 (MQ=255)                                                                                                            aTCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                    >  2:760590/1‑150 (MQ=255)                                                                                                             tctcGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                    >  1:749764/1‑149 (MQ=255)                                                                                                                cGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAcc                               >  1:231253/1‑151 (MQ=255)                                                                                                                cGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAc                                <  2:20622/150‑1 (MQ=255)                                                                                                                         ggTTGATGTCCGCGGTTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTgcg                       <  1:398111/150‑1 (MQ=255)                                                                                                                          gTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGcc                     >  2:453398/1‑151 (MQ=255)                                                                                                                               tGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGtt                 <  2:392928/150‑1 (MQ=255)                                                                                                                                  ccgccgGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGt                  <  1:453347/146‑1 (MQ=255)                                                                                                                                    gccgGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTc            >  1:548133/1‑150 (MQ=255)                                                                                                                                      cgGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATc         >  1:599434/1‑151 (MQ=255)                                                                                                                                       gttGCTCCCCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATc         <  1:492758/148‑1 (MQ=255)                                                                                                                                              cgcccgccTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGtt  <  1:417535/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTT  >  NC_000913/3243280‑3243570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑