Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,286,334	T→C	L22P (CTT→CCT)	agaI →	galactosamine‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,286,334	0	T	C	100.0%	179.6 / NA	50	L22P (CTT→CCT)	agaI	galactosamine‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/20);  total (30/20)

AAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCC  >  NC_000913/3286194‑3286480                                                                                                                                             |                                                                                                                                                   aaaaGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGtt                                                                                                                                          >  1:56240/1‑151 (MQ=255)       gcATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAAc                                                                                                                                    >  1:151574/1‑151 (MQ=255)        cATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACt                                                                                                                                   >  1:676467/1‑151 (MQ=255)                gCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGc                                                                                                                            <  1:13213/150‑1 (MQ=255)                     tAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTa                                                                                                                        >  1:257031/1‑149 (MQ=255)                     tAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTa                                                                                                                        >  2:480634/1‑149 (MQ=255)                           gCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACg                                                                                                                <  1:277675/151‑1 (MQ=255)                                aCTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGcc                                                                                                            >  2:332316/1‑150 (MQ=255)                                 cTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGcc                                                                                                            >  2:162143/1‑149 (MQ=255)                                   tttGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAg                                                                                                          <  2:473276/149‑1 (MQ=255)                                   tttGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGc                                                                                                         <  1:21818/150‑1 (MQ=255)                                     tgtgCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGa                                                                                                       >  1:651969/1‑150 (MQ=255)                                      gtgCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAat                                                                                                     <  2:633949/151‑1 (MQ=255)                                        gCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAatat                                                                                                   <  2:56271/151‑1 (MQ=255)                                          tctctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtattt                                                                                                 >  2:483962/1‑151 (MQ=255)                                                ttgtttgtTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGc                                                                                           >  1:521923/1‑151 (MQ=255)                                                 tgtttgttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGcc                                                                                          >  1:587351/1‑151 (MQ=255)                                                     tgttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTg                                                                                       >  1:395756/1‑150 (MQ=255)                                                      gttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGAt                                                                                     <  1:554109/151‑1 (MQ=255)                                                           gCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGt                                                                                 >  2:693364/1‑150 (MQ=255)                                                           gCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTa                                                                                <  2:573898/151‑1 (MQ=255)                                                            cATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGt                                                                                 >  1:130278/1‑149 (MQ=255)                                                             aTTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGc                                                                              >  2:348752/1‑151 (MQ=255)                                                                 ggCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAAc                                                                          <  2:477632/151‑1 (MQ=255)                                                                     ttttGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGa                                                                       >  2:361470/1‑150 (MQ=255)                                                                       ttGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAAt                                                                     <  2:567894/150‑1 (MQ=255)                                                                        tGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGc                                                                   >  2:297490/1‑151 (MQ=255)                                                                            aaTGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTg                                                               >  1:115619/1‑151 (MQ=255)                                                                            aaTGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTg                                                               >  1:13085/1‑151 (MQ=255)                                                                               ggAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGAtt                                                            <  2:729447/151‑1 (MQ=255)                                                                                       gCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGc                                                    >  2:94752/1‑151 (MQ=255)                                                                                         aCTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCg                                                   >  2:216498/1‑150 (MQ=255)                                                                                          cTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGAc                                                 >  1:687665/1‑151 (MQ=255)                                                                                            gCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCg                                               <  2:760136/151‑1 (MQ=255)                                                                                            gCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCg                                               <  1:661234/151‑1 (MQ=255)                                                                                                  ggtggtGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGcc                                          >  2:500800/1‑150 (MQ=255)                                                                                                         ccTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCAtt                                  >  1:397313/1‑151 (MQ=255)                                                                                                              ttACTTAAGGAATTTCATCCTGTGCACACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACt                               >  2:424088/1‑149 (MQ=255)                                                                                                                   tAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTa                         <  1:711829/150‑1 (MQ=255)                                                                                                                   tAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTa                         <  1:693290/150‑1 (MQ=255)                                                                                                                     aGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATc                       >  1:503921/1‑150 (MQ=255)                                                                                                                     aGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCa                      <  1:667019/151‑1 (MQ=255)                                                                                                                      ggAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCAt                     <  2:2052/151‑1 (MQ=255)                                                                                                                         aTTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTAt                  >  1:316489/1‑151 (MQ=255)                                                                                                                          tttCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATc                 >  1:310428/1‑151 (MQ=255)                                                                                                                            tCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCt                <  2:299026/150‑1 (MQ=255)                                                                                                                            tCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCt                <  2:257064/150‑1 (MQ=255)                                                                                                                               tCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGt             >  1:538048/1‑150 (MQ=255)                                                                                                                                       aaaCCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAAt    <  1:170848/151‑1 (MQ=255)                                                                                                                                         aCCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATcc  >  1:605028/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                   AAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCC  >  NC_000913/3286194‑3286480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑