Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	56,483	C→T	P209P (CCG→CCA)	lptD ←	LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	56,483	0	C	T	100.0%	166.9 / NA	45	P209P (CCG→CCA)	lptD	LPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/25);  total (20/25)

GTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATCGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGAATGGTATAGCGG  >  NC_000913/56337‑56630                                                                                                                                                   |                                                                                                                                                    gtggCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTa                                                                                                                                                 <  1:110528/151‑1 (MQ=255)   ggCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTAc                                                                                                                                                <  2:195696/150‑1 (MQ=255)     cATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCgg                                                                                                                                             <  1:163599/151‑1 (MQ=255)      aTCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGa                                                                                                                                            <  1:21599/151‑1 (MQ=255)            atTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGGTTGGTACCGGACCCAcc                                                                                                                                      >  2:4511/1‑151 (MQ=255)                  cgcgATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAg                                                                                                                                <  1:564487/151‑1 (MQ=255)                        gTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGGGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCg                                                                                                                          >  1:538863/1‑151 (MQ=255)                          tCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGtt                                                                                                                        >  1:536996/1‑151 (MQ=255)                           ccAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGGGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGGGtt                                                                                                                        >  1:30599/1‑150 (MQ=255)                             aGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTcc                                                                                                                      >  2:703851/1‑150 (MQ=255)                             aGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTcc                                                                                                                      >  2:573369/1‑150 (MQ=255)                                aaTATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTcc                                                                                                                      >  2:273856/1‑147 (MQ=255)                                 atatGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTcc                                                                                                                      >  1:45979/1‑146 (MQ=255)                                   atGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGAtct                                                                                                                <  2:653824/150‑1 (MQ=255)                                           tAGAACTCAAAGTATTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACt                                                                                                        <  2:616710/150‑1 (MQ=255)                                            aGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACtt                                                                                                       <  1:504688/150‑1 (MQ=255)                                             gAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGt                                                                                                     >  1:293279/1‑151 (MQ=255)                                              aaCTCAAAGTAGTTGGTGGTGGTGTACTTGGCGATCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGtt                                                                                                    >  2:432106/1‑151 (MQ=255)                                               aCTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGttc                                                                                                   <  1:32094/151‑1 (MQ=255)                                                  cAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGACAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGttct                                                                                                  <  2:774993/149‑1 (MQ=255)                                                   aaaGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTcg                                                                                               <  1:104129/151‑1 (MQ=255)                                                            ggtggtggtGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATg                                                                                       <  1:49355/150‑1 (MQ=255)                                                               ggtggtgTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGaat                                                                                    <  1:764265/150‑1 (MQ=255)                                                                      tACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGc                                                                            >  2:612974/1‑151 (MQ=255)                                                                       aCTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGct                                                                           >  2:645306/1‑151 (MQ=255)                                                                                      acAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAGTAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTGTTCGCGGTCATGAATAATTTCGCTACCTACCACGcgc                                                              >  1:106603/2‑147 (MQ=255)                                                                                         aGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGgt                                                         <  2:482946/151‑1 (MQ=255)                                                                                                  agCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAAcc                                                >  1:103978/1‑151 (MQ=255)                                                                                                   gCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAAccc                                               <  2:578192/151‑1 (MQ=255)                                                                                                     gACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCgg                                             >  2:72713/1‑151 (MQ=255)                                                                                                     gACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCgg                                             <  1:358598/151‑1 (MQ=255)                                                                                                      aCGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGc                                            <  2:490316/151‑1 (MQ=255)                                                                                                              cacccaccGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACagg                                     <  2:537048/150‑1 (MQ=255)                                                                                                                 ccaccGGCAACTGCAAATAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGt                                 >  2:133064/1‑151 (MQ=255)                                                                                                                               aaaTAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTAt                   >  1:697903/1‑151 (MQ=255)                                                                                                                                aaTAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTGATc                  <  1:500464/151‑1 (MQ=255)                                                                                                                                 aTAGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATcc                 <  2:46010/151‑1 (MQ=255)                                                                                                                                   aGGGGCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCa                <  1:315435/150‑1 (MQ=255)                                                                                                                                    ggggCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGa              <  1:445895/151‑1 (MQ=255)                                                                                                                                     gggCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGaa             <  1:496854/151‑1 (MQ=255)                                                                                                                                      ggCTATAAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGAAt            >  1:558415/1‑151 (MQ=255)                                                                                                                                           taAAAGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGAATGGtat       >  1:84755/1‑151 (MQ=255)                                                                                                                                              aaGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGAATGGTATAGc    >  1:218837/1‑151 (MQ=255)                                                                                                                                              aaGATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGAATGGTATAGc    <  1:396137/151‑1 (MQ=255)                                                                                                                                                gATTGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGAATGGTATAGCgg  <  2:445600/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGCTATAAAAGATCGGTACCGGACCCACCTTAAAGCGGGCGTTCCAGATCTCCGCAACTTGTTCTTCGCGGTCATGAATAATTTCGCTACCTACCACGCTCCAGGTGTCAGAACCCGGCAGACAGGAGGTAAAGCTACCGTTATCCAGAATGGTATAGCGG  >  NC_000913/56337‑56630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑