| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 373,704 | G→A | V262M (GTG→ATG) | mhpF → | acetaldehyde‑CoA dehydrogenase II, NAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 373,704 | 0 | G | A | 97.7% | 154.5 / NA | 43 | V262M (GTG→ATG) | mhpF | acetaldehyde‑CoA dehydrogenase II, NAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (16/26); total (17/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGC > NC_000913/373556‑373850 | atTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATg < 2:230455/151‑1 (MQ=255) gacgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAAt > 2:274486/1‑151 (MQ=255) aGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTcc < 2:687122/151‑1 (MQ=255) gCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCg < 2:354867/151‑1 (MQ=255) cacaAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACt < 2:478928/151‑1 (MQ=255) atatCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATTGCCTTAAACAGCGCGGGCAGTTTGAAGTTATACCGCAGGATAAACCGGTCAATTTAACGGGCATGGGGCAAATCTCCCGGCTGAAAACAg > 2:414818/1‑150 (MQ=255) atatCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGc < 1:114408/151‑1 (MQ=255) atatCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCCCCTGAAACAACGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACa > 2:19508/1‑149 (MQ=255) cGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTc < 1:574574/151‑1 (MQ=255) aGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTctgg < 2:521948/151‑1 (MQ=255) tcaatcaaTGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGGCAATTTACCGGGCATGGGGCAATTCTCCGGGCTGAAAACAGCGGTctggctgg > 1:581473/1‑151 (MQ=255) caatcaatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTctggctgg > 1:616318/1‑150 (MQ=255) caatcaatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTctggctgg > 2:652481/1‑150 (MQ=255) aatcaatGAAATGGCTGAGGCGGTGCCGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAAAAGCGGTctggctgg > 2:155139/1‑149 (MQ=255) caatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTc < 2:461979/151‑1 (MQ=255) aatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCg < 1:404112/151‑1 (MQ=255) gAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGaa < 1:514015/150‑1 (MQ=255) aaTGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgc < 2:749504/151‑1 (MQ=255) aTGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcg < 2:509455/151‑1 (MQ=255) tGAGGCGGTGCAAGCTTACGTACCGGGTTATCGCCTGAAAAAACGCGTGCAGGTTGAAGTTATCCCGCAGGATAAACCGGGCAATTTACCGGGCGTGGGGCAATTCTCCGGGCTGAAAAAAACGGGCTGGCTGGAAGTCGAAGgggcagcg > 1:755731/1‑151 (MQ=255) aGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctgcc < 1:59075/151‑1 (MQ=255) ggCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctgcct < 1:183471/151‑1 (MQ=255) tACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCtgcctgcc < 2:613920/150‑1 (MQ=255) gTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATg < 1:519483/151‑1 (MQ=255) cGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCggg < 2:378848/151‑1 (MQ=255) cGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCg > 1:417863/1‑151 (MQ=255) ccTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGAc < 2:495849/151‑1 (MQ=255) aaaCAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTa < 2:82668/151‑1 (MQ=255) aaCAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTAt > 1:570745/1‑151 (MQ=255) gcgcGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACt < 2:219866/151‑1 (MQ=255) gcgTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTc > 1:432701/1‑151 (MQ=255) gCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGt < 2:636643/151‑1 (MQ=255) tGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCt < 1:274453/150‑1 (MQ=255) tGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTg > 1:463378/1‑151 (MQ=255) tGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTg > 1:530883/1‑151 (MQ=255) tGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTg > 1:39537/1‑151 (MQ=255) aaGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGc < 2:142422/151‑1 (MQ=255) aCCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGcc < 2:175973/151‑1 (MQ=255) aaTTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCAc > 2:732164/1‑151 (MQ=255) aTTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACt < 2:417914/151‑1 (MQ=255) aCCGGGCATGGGGCAATTCTCCGTACTGAAAACATCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTACCAGTGCGCTGGCGACAGCGTAAAAAATGGCCCAGTCACTGgcg < 1:301932/151‑1 (MQ=255) gggCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcgc > 1:80110/1‑151 (MQ=255) gggCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcgc > 1:287890/1‑151 (MQ=255) | ATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGC > NC_000913/373556‑373850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |