Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,363,151 | T→C | G115G (GGT→GGC) | yhcD → | putative outer membrane fimbrial subunit usher protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,363,151 | 0 | T | C | 96.7% | 205.5 / ‑6.6 | 61 | G115G (GGT→GGC) | yhcD | putative outer membrane fimbrial subunit usher protein |
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (40/19); total (41/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGAGCAGCCGT > NC_000913/3363005‑3363295 | aaTGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCAcc > 2:407698/1‑150 (MQ=255) aaTGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCAcc > 1:289170/1‑150 (MQ=255) gCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAAt < 1:667219/151‑1 (MQ=255) gAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATc > 1:520413/1‑150 (MQ=255) aaaaaaaaGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATcac > 1:67385/1‑151 (MQ=255) aaaaaaGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCacac > 2:223784/1‑151 (MQ=255) aaaaaaGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCacac > 1:17278/1‑151 (MQ=255) aaaaaGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACAcc > 2:278222/1‑151 (MQ=255) aGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTa < 1:96727/151‑1 (MQ=255) cAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCt > 2:154505/1‑151 (MQ=255) ttGCTACGCGATTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAAcc > 1:573192/1‑150 (MQ=255) tGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCa < 1:366817/150‑1 (MQ=255) cgcTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGt > 2:11392/1‑151 (MQ=255) gatgaAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGat > 2:300908/1‑150 (MQ=255) gaAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGAtatt < 2:19051/150‑1 (MQ=255) gaAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGAtatt > 2:381531/1‑150 (MQ=255) gaAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGAtatt < 2:746930/150‑1 (MQ=255) aaaTGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATc < 1:289148/151‑1 (MQ=255) ggCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGttt < 2:312487/151‑1 (MQ=255) gCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTc < 1:635712/151‑1 (MQ=255) aCATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcgcc < 1:21147/151‑1 (MQ=255) aCATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcacc > 1:198712/1‑151 (MQ=255) cATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcacc > 2:611640/1‑150 (MQ=255) tttGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcaccac > 1:277403/1‑150 (MQ=255) ttGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACcaca < 1:534866/150‑1 (MQ=255) tGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAgg > 2:84966/1‑151 (MQ=255) tGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAgg > 1:572648/1‑151 (MQ=255) cAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTc > 2:401619/1‑151 (MQ=255) cATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCAt > 2:19206/1‑151 (MQ=255) tCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAggg > 1:564071/1‑151 (MQ=255) caacaaGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTgg > 1:50704/1‑151 (MQ=255) caacaaGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTgg < 2:737532/151‑1 (MQ=255) aaGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAtct > 2:503910/1‑151 (MQ=255) gCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTca < 1:170314/151‑1 (MQ=255) gTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACaa > 1:493023/1‑150 (MQ=255) ttAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACg > 1:623633/1‑151 (MQ=255) aactCCTGCTGAAGAAAAAAATGGCTGTTACAACTATATATTGTCCTACCCGGGTACCATAACCACAACATAACCTAACCAACAAGCATTAGATATAATCGTTGCACAAAAGGCGATCATTCCCATACAGTCGGATCTCACAAACgcagct < 1:299045/148‑2 (MQ=255) aTTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACgcagca > 1:486473/1‑150 (MQ=255) cTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGAt > 1:118884/1‑109 (MQ=255) cTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGAt < 2:118916/109‑1 (MQ=255) tGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACtggtgg > 2:540686/1‑150 (MQ=255) tGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACtggtgg > 1:415774/1‑150 (MQ=255) aagaaAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGaa > 2:496154/1‑150 (MQ=255) aagaaAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAAc > 1:9714/1‑151 (MQ=255) aaaaaaaTGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTGCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAg < 1:679176/150‑1 (MQ=255) aaaaaTGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAgctg > 2:650966/1‑151 (MQ=255) gTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTa < 1:650908/149‑1 (MQ=255) gTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTa < 2:583955/149‑1 (MQ=255) ttACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAac > 2:37944/1‑151 (MQ=255) aCGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAact < 2:660908/150‑1 (MQ=255) tatataTTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTActctc > 2:250963/1‑151 (MQ=255) atatatTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTActctc < 2:423828/150‑1 (MQ=255) tatatTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTActctct > 1:108074/1‑150 (MQ=255) tatatTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCtg > 1:49166/1‑151 (MQ=255) tatTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCtgat > 2:48105/1‑151 (MQ=255) atTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCtgatg > 2:291764/1‑151 (MQ=255) gTCCTACCTGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGagc > 1:220705/1‑151 (MQ=255) cTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGagcagc > 1:189137/1‑151 (MQ=255) cTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGagcagc > 2:166580/1‑151 (MQ=255) cccGGGCACCACAAACACACCATTACCTAACCAGGAAGCATTAGATATTATCGTTTCTCCACAGGCCATCATTCCCAAGGGGGTCGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCGCTGATGagcagtc < 1:236134/149‑3 (MQ=255) ccGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGAGCAGCCGt > 1:712271/1‑150 (MQ=255) | AATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGAGCAGCCGT > NC_000913/3363005‑3363295 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |