Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,508,245	T→C	T116A (ACG→GCG)	php ←	phosphotriesterase homology protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,245	0	T	C	100.0%	141.9 / NA	41	T116A (ACG→GCG)	php	phosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/24);  total (17/24)

GCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAG  >  NC_000913/3508105‑3508392                                                                                                                                             |                                                                                                                                                    gatGAACTGCGGATGCGTGGAGATCAGTCGTCCGGTCTGGTTATGAGCCAGCTCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGACGATCTCCGCGATGATACCGGCTTTCAGCTCCGCGCCGTCGTTa                                                                                                                                           <  1:448654/149‑1 (MQ=255)        gacgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGtt                                                                                                                                    <  1:126003/151‑1 (MQ=255)         acgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTc                                                                                                                                   <  1:597704/151‑1 (MQ=255)                 gTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCa                                                                                                                            <  1:764891/150‑1 (MQ=255)                                          tATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCt                                                                                                   <  1:549590/150‑1 (MQ=255)                                           aTGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGc                                                                                                 >  1:410308/1‑151 (MQ=255)                                             gcgcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCAc                                                                                               <  2:132552/151‑1 (MQ=255)                                                    ggcagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTCCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCgg                                                                                        >  1:681689/2‑151 (MQ=255)                                                      cagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                                                      >  2:558376/1‑151 (MQ=255)                                                      cagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                                                      <  1:584792/151‑1 (MQ=255)                                                       agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTc                                                                                     <  1:98406/151‑1 (MQ=255)                                                       agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTc                                                                                     >  1:56919/1‑151 (MQ=255)                                                       agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTc                                                                                     >  1:450316/1‑151 (MQ=255)                                                                    aCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCg                                                                        >  1:329322/1‑151 (MQ=255)                                                                     ccTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCgg                                                                       >  2:361657/1‑151 (MQ=255)                                                                       ttCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCggg                                                                      >  1:119703/1‑150 (MQ=255)                                                                       ttCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCggg                                                                      >  1:124684/1‑150 (MQ=255)                                                                             tCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAc                                                                <  2:243117/150‑1 (MQ=255)                                                                              ccAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcgc                                                              <  1:203976/151‑1 (MQ=255)                                                                              ccAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAc                                                                <  1:486730/149‑1 (MQ=255)                                                                                aGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGt                                                            <  2:3618/151‑1 (MQ=255)                                                                                      gTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGt                                                      >  2:542985/1‑151 (MQ=255)                                                                                                cTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGt                                            >  2:66231/1‑151 (MQ=255)                                                                                                  tCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTAc                                          >  1:304631/1‑151 (MQ=255)                                                                                                   cGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTAc                                          >  2:500581/1‑150 (MQ=255)                                                                                                        gTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGcca                                    <  2:758755/151‑1 (MQ=255)                                                                                                          gCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGccacc                                  >  2:626750/1‑151 (MQ=255)                                                                                                                  ccGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCcaccac                                <  1:290218/145‑1 (MQ=255)                                                                                                                  ccGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACg                               <  2:578569/146‑1 (MQ=255)                                                                                                                  ccGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGtt                             <  2:378031/148‑1 (MQ=255)                                                                                                                  ccGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGAt                          <  2:251096/151‑1 (MQ=255)                                                                                                                   cgcgATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATc                         >  2:409911/1‑151 (MQ=255)                                                                                                                         gatCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGt                    <  1:738037/150‑1 (MQ=255)                                                                                                                            cccGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTcg                <  2:486448/151‑1 (MQ=255)                                                                                                                                 cTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCAt           >  1:305539/1‑151 (MQ=255)                                                                                                                                  tttCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAACAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCAtt          >  1:209315/1‑151 (MQ=255)                                                                                                                                    tCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTa         <  1:415359/150‑1 (MQ=255)                                                                                                                                     cAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACa       <  2:639865/151‑1 (MQ=255)                                                                                                                                          ccGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCaa   <  1:183460/150‑1 (MQ=255)                                                                                                                                          ccGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCa    <  1:108582/149‑1 (MQ=255)                                                                                                                                          ccGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAg  <  1:225091/151‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                    GCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAG  >  NC_000913/3508105‑3508392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑