Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,554,511	T→C	L476P (CTG→CCG)	malT →	mal regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,554,511	0	T	C	100.0%	163.0 / NA	47	L476P (CTG→CCG)	malT	mal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/25);  total (22/25)

GAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTCT  >  NC_000913/3554363‑3554654                                                                                                                                                     |                                                                                                                                                gAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCgg                                                                                                                                               <  2:468037/151‑1 (MQ=255)  aaGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCgg                                                                                                                                               >  2:693767/1‑150 (MQ=255)  aaGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCgg                                                                                                                                               >  1:256869/1‑150 (MQ=255)        acacCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCt                                                                                                                                        >  2:455967/1‑151 (MQ=255)           cccTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAAAATTTAACGCTCTGCGCGCCCAGGTTGCGATTAACGATGGTAATCCGGATTAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgc                                                                                                                                      <  2:188876/150‑1 (MQ=255)            ccTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgccg                                                                                                                                    <  1:646937/151‑1 (MQ=255)              tGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgccgcc                                                                                                                                  >  1:420266/1‑151 (MQ=255)               gCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgccgcc                                                                                                                                  <  2:43374/150‑1 (MQ=255)               gCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGccgccg                                                                                                                                 <  1:455916/151‑1 (MQ=255)                    cccGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTg                                                                                                                            >  2:320281/1‑151 (MQ=255)                     ccGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTgg                                                                                                                           >  2:104675/1‑151 (MQ=255)                               cATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGcc                                                                                                                 <  2:291021/151‑1 (MQ=255)                               cATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGcc                                                                                                                 >  1:613510/1‑151 (MQ=255)                                 tGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGc                                                                                                               >  2:456470/1‑151 (MQ=255)                                  gAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGc                                                                                                               >  1:649766/1‑150 (MQ=255)                                     aTCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATtg                                                                                                           >  2:328978/1‑151 (MQ=255)                                       cAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATtgtg                                                                                                         >  1:309091/1‑151 (MQ=255)                                       cAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATtgtg                                                                                                         <  2:734582/151‑1 (MQ=255)                                         aGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGc                                                                                                       <  1:598441/151‑1 (MQ=255)                                                cAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCg                                                                                                <  1:360058/151‑1 (MQ=255)                                                          acCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTg                                                                                      <  1:119432/151‑1 (MQ=255)                                                            cATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGaa                                                                                    <  2:178857/151‑1 (MQ=255)                                                               gCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGtg                                                                                 <  2:400803/151‑1 (MQ=255)                                                                    cAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGctgca                                                                            <  1:449538/151‑1 (MQ=255)                                                                            aaCGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAg                                                                    <  1:188940/151‑1 (MQ=255)                                                                              cGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGc                                                                  <  1:213232/151‑1 (MQ=255)                                                                               gCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCg                                                                 <  2:119786/151‑1 (MQ=255)                                                                               gCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCg                                                                 >  1:655980/1‑151 (MQ=255)                                                                                      gcgcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGAc                                                          <  2:130174/151‑1 (MQ=255)                                                                                      gcgcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGAc                                                          >  1:203746/1‑151 (MQ=255)                                                                                              gTGGCGATTAACGATGGTAATCCAGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCAc                                                  <  2:140725/151‑1 (MQ=255)                                                                                               tGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACt                                                 >  2:490712/1‑151 (MQ=255)                                                                                                 gCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTgg                                               <  1:76378/151‑1 (MQ=255)                                                                                                        aCGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAAt                                        >  1:333833/1‑151 (MQ=255)                                                                                                           aTGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATgc                                      >  1:459237/1‑150 (MQ=255)                                                                                                              gTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATgcagca                                  <  2:491920/151‑1 (MQ=255)                                                                                                                   ccGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCg                             <  1:442588/151‑1 (MQ=255)                                                                                                                    cGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAAcc                              <  2:723373/149‑1 (MQ=255)                                                                                                                      gATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGaa                           >  2:486411/1‑150 (MQ=255)                                                                                                                         gAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGAATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAAc                          >  2:574086/1‑148 (MQ=255)                                                                                                                           aGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATg                     >  1:302676/1‑151 (MQ=255)                                                                                                                              ggAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGc                   >  1:350366/1‑150 (MQ=255)                                                                                                                                  cGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGcc              <  2:43394/151‑1 (MQ=255)                                                                                                                                    gCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGcc              >  2:46403/1‑149 (MQ=255)                                                                                                                                       ggCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGc           <  2:302567/149‑1 (MQ=255)                                                                                                                                          aaaaCTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGAt      >  1:199891/1‑151 (MQ=255)                                                                                                                                                ggCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTCt  <  2:431287/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                GAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTCT  >  NC_000913/3554363‑3554654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑