Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,691	T→C	V18A (GTT→GCT)	sbmA →	peptide antibiotic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,691	0	T	C	100.0%	170.9 / NA	49	V18A (GTT→GCT)	sbmA	peptide antibiotic transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/21);  total (28/21)

TCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTT  >  NC_000913/396543‑396837                                                                                                                                                     |                                                                                                                                                   tCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGctt                                                                                                                                                  >  2:320591/1‑151 (MQ=255)     aaTGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGc                                                                                                                                              <  1:550725/151‑1 (MQ=255)      aTGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCa                                                                                                                                             <  2:552100/151‑1 (MQ=255)          gCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCattga                                                                                                                                         <  1:619352/151‑1 (MQ=255)                 cATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGt                                                                                                                                  <  1:693157/151‑1 (MQ=255)                      ggCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTAtct                                                                                                                             >  1:341489/1‑151 (MQ=255)                       gCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTAtctt                                                                                                                            <  2:293474/151‑1 (MQ=255)                         tGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTAtcttct                                                                                                                          >  2:294313/1‑151 (MQ=255)                           aCGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTgg                                                                                                                        >  1:440924/1‑151 (MQ=255)                           aCGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTgg                                                                                                                        <  2:549115/151‑1 (MQ=255)                            cGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGc                                                                                                                       <  1:381734/151‑1 (MQ=255)                             gCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCa                                                                                                                      <  1:320558/151‑1 (MQ=255)                                ttGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTAtct                                                                                                                             <  2:750153/141‑1 (MQ=255)                                ttGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTAtct                                                                                                                             >  1:750078/1‑141 (MQ=255)                                ttGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCaa                                                                                                                     <  1:631723/149‑1 (MQ=255)                                      cGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTgg                                                                                                             <  1:270971/151‑1 (MQ=255)                                             gCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGAc                                                                                                      >  1:649345/1‑151 (MQ=255)                                                gTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAGCGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTgg                                                                                                   >  2:330334/1‑151 (MQ=255)                                                 ttAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTggg                                                                                                  >  1:157298/1‑151 (MQ=255)                                                  tAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGt                                                                                                 >  2:335341/1‑151 (MQ=255)                                                   aaTACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTg                                                                                                >  1:91531/1‑151 (MQ=255)                                                        acaGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGcgcg                                                                                           >  2:773641/1‑151 (MQ=255)                                                                tgttAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCAcc                                                                                    >  2:403798/1‑150 (MQ=255)                                                                       aCGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTc                                                                            <  2:171560/151‑1 (MQ=255)                                                                         gATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCg                                                                          >  1:150657/1‑151 (MQ=255)                                                                          aTAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCgg                                                                         >  2:536052/1‑151 (MQ=255)                                                                            aagaagTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCggg                                                                        <  1:9248/150‑1 (MQ=255)                                                                                   tAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATccc                                                                >  2:703105/1‑151 (MQ=255)                                                                                   tAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATccc                                                                >  1:38925/1‑151 (MQ=255)                                                                                          aGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAg                                                          >  1:542/1‑150 (MQ=255)                                                                                              cATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGGGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTa                                                           >  2:635894/1‑145 (MQ=255)                                                                                                tatGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCcgcg                                                   >  2:543514/1‑151 (MQ=255)                                                                                                   gTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCcgcgcg                                                 <  2:700168/150‑1 (MQ=255)                                                                                                     ttAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGttt                                              >  2:199170/1‑151 (MQ=255)                                                                                                     ttAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGttt                                              >  1:683614/1‑151 (MQ=255)                                                                                                            ttttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCg                                       >  2:451560/1‑151 (MQ=255)                                                                                                             tttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTc                                        <  1:443037/149‑1 (MQ=255)                                                                                                              ttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGTCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGtt                                     >  1:647301/1‑151 (MQ=255)                                                                                                                 tCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTgg                                   <  2:681792/150‑1 (MQ=255)                                                                                                                  cccAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGa                                  <  1:294280/150‑1 (MQ=255)                                                                                                                   ccAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGAtt                                <  1:756284/151‑1 (MQ=255)                                                                                                                   ccAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGAtt                                >  1:423584/1‑151 (MQ=255)                                                                                                                          cGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGGGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAt                         >  2:635497/1‑151 (MQ=255)                                                                                                                                     tttCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCtt              >  1:465703/1‑151 (MQ=255)                                                                                                                                     tttCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCtt              <  2:32268/150‑1 (MQ=255)                                                                                                                                          ctcGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtact           <  2:567887/149‑1 (MQ=255)                                                                                                                                            cGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtactac         <  1:670902/149‑1 (MQ=255)                                                                                                                                             ggCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACAtt      >  1:188788/1‑151 (MQ=255)                                                                                                                                             ggCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACAtt      >  2:768228/1‑151 (MQ=255)                                                                                                                                                 ttCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGttt  >  1:529101/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTT  >  NC_000913/396543‑396837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑