Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,603,920 T→C E108E (GAA→GAG)  ftsY ← Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,603,9200TC100.0% 153.6 / NA 42E108E (GAA→GAG) ftsYSignal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/17);  total (25/17)

TTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTCAG  >  NC_000913/3603775‑3604065
                                                                                                                                                 |                                                                                                                                                 
tttCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGAt                                                                                                                                              >  1:750119/1‑151 (MQ=255)
    cAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGcc                                                                                                                                          >  2:438016/1‑151 (MQ=255)
         ctTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTgg                                                                                                                                     >  1:605179/1‑151 (MQ=255)
            cgtcgGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTc                                                                                                                                  >  2:377350/1‑151 (MQ=255)
             gtcgGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTcc                                                                                                                                 <  1:774885/151‑1 (MQ=255)
                  tAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGt                                                                                                                            >  2:550105/1‑151 (MQ=255)
                         ttcttTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTtcttct                                                                                                                     >  2:651280/1‑151 (MQ=255)
                          tcttTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTtcttct                                                                                                                     >  2:565159/1‑150 (MQ=255)
                              tAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGAc                                                                                                                >  2:270637/1‑151 (MQ=255)
                               aGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGAcc                                                                                                               >  1:741068/1‑151 (MQ=255)
                                          cttcCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCg                                                                                                        <  1:415496/147‑1 (MQ=255)
                                          cttcCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCtg                                                                                                    >  1:328916/1‑151 (MQ=255)
                                           ttcCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCgg                                                                                                       <  1:446794/147‑1 (MQ=255)
                                            tcCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCtgtg                                                                                                  >  2:197627/1‑151 (MQ=255)
                                                     tCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACtt                                                                                         >  2:262128/1‑151 (MQ=255)
                                                      cGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTc                                                                                        >  2:9925/1‑151 (MQ=255)
                                                      cGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTc                                                                                        >  1:717675/1‑151 (MQ=255)
                                                                 aCGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCt                                                                             >  2:452671/1‑151 (MQ=255)
                                                                      ttcagcttcagcCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCt                                                                        >  1:60455/1‑151 (MQ=255)
                                                                      ttCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTgcgc                                                                         <  2:764432/150‑1 (MQ=255)
                                                                                  cTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTc                                                             >  2:129349/1‑150 (MQ=255)
                                                                                     ccACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCa                                                         <  2:107962/151‑1 (MQ=255)
                                                                                     ccACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCa                                                         <  1:439916/151‑1 (MQ=255)
                                                                                       aCTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCCGGCTGCGCTTTTTAACTTTCAGCAAc                                                       >  1:183417/1‑151 (MQ=255)
                                                                                             ctGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTc                                                 >  2:391571/1‑151 (MQ=255)
                                                                                               ggCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGtt                                                  <  1:437965/148‑1 (MQ=255)
                                                                                                  gAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGGCCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTg                                             <  2:128697/150‑1 (MQ=255)
                                                                                                         cTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCcac                                     >  2:465475/1‑151 (MQ=255)
                                                                                                          ttCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCcac                                     >  1:718125/1‑150 (MQ=255)
                                                                                                          ttCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCcac                                     >  1:660949/1‑150 (MQ=255)
                                                                                                            cGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACaa                                   <  1:577629/150‑1 (MQ=255)
                                                                                                            cGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACaa                                   <  2:384791/150‑1 (MQ=255)
                                                                                                            cGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAAc                                  <  1:245830/151‑1 (MQ=255)
                                                                                                                      gTCTTCCGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGgcagca                        <  2:644218/151‑1 (MQ=255)
                                                                                                                           ccGGCAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAg                    <  2:750194/150‑1 (MQ=255)
                                                                                                                               cAACGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCAGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTcc               <  2:47900/151‑1 (MQ=255)
                                                                                                                                aaCGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTcc               >  1:159752/1‑150 (MQ=255)
                                                                                                                                aaCGGCAGCTCTTCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCg              <  2:328949/151‑1 (MQ=255)
                                                                                                                                           ttCACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTc    >  2:194159/1‑150 (MQ=255)
                                                                                                                                            acACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGATGCGCAACGACTTCAGCTTCAGGCTGCGCTTTTTCACTTGCAGCAACCTGTTCAGTGACTTCCACAACGTCTGCAGCCAAAGATTCCGCCTCGGCTTc    >  1:475924/2‑149 (MQ=255)
                                                                                                                                             cACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTc    <  1:651241/148‑1 (MQ=255)
                                                                                                                                             cACGCTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTCAg  <  2:605236/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTCAG  >  NC_000913/3603775‑3604065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑