Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,626,537	T→C	G89G (GGA→GGG)	yhhJ ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,626,537	0	T	C	100.0%	152.7 / NA	43	G89G (GGA→GGG)	yhhJ	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/21);  total (22/21)

TCTGGATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGTAACGG  >  NC_000913/3626392‑3626682                                                                                                                                                  |                                                                                                                                                  tCTGGATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGc                                                                                                                                              >  1:2619/1‑151 (MQ=255)    ggATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGcatc                                                                                                                                           >  1:386776/1‑151 (MQ=255)             cATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATc                                                                                                                                  >  1:330948/1‑151 (MQ=255)                tGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATcgg                                                                                                                                <  1:725989/150‑1 (MQ=255)                  ccGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATcggcgg                                                                                                                             >  2:610264/1‑151 (MQ=255)                   cGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGt                                                                                                                            <  2:495954/151‑1 (MQ=255)                                gCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCgg                                                                                                                >  1:303768/1‑150 (MQ=255)                                   cATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTgg                                                                                                             <  1:472/150‑1 (MQ=255)                                      gcgcGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGTGGCaa                                                                                                          >  2:237989/1‑150 (MQ=255)                                            ggCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCAc                                                                                                   <  2:274972/151‑1 (MQ=255)                                             gCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATTATCTCCCGTGGCaaaaaa                                                                                                      >  1:625226/1‑146 (MQ=255)                                               aTCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGa                                                                                                <  1:263143/151‑1 (MQ=255)                                                 cGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGTGGCAAAAACCACgg                                                                                                 >  1:515734/1‑148 (MQ=255)                                                   aCGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATTTCCCGGGGCAAAAACCACgg                                                                                                 >  2:395611/1‑146 (MQ=255)                                                     gTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAg                                                                                          <  2:235813/151‑1 (MQ=255)                                                     gTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGGGATTATCTCCGGGGGCAAAAAacac                                                                                                   >  2:11943/1‑142 (MQ=255)                                                         aCCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGGGATCATCTCCCGGGGCAAAAAACACGGACGAt                                                                                            >  1:665934/1‑145 (MQ=255)                                                             gAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTg                                                                                    <  1:135581/149‑1 (MQ=255)                                                                         ggCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAACCCACGGACGATAGAAGCTGTTAACGATCCGGtt                                                                      <  2:395255/151‑1 (MQ=255)                                                                         ggCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCAGCAGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGCTCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGtt                                                                      <  1:325265/151‑1 (MQ=255)                                                                              ccGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGAt                                                                  <  2:464233/150‑1 (MQ=255)                                                                                   gAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAACCACGGGCGATAGAAGCTGTTAAAGATCCGGTTCGATAAATGc                                                            >  2:561654/1‑151 (MQ=255)                                                                                       aCATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATTTCCCGTGGCAAAAACCACGAGCGATAAAAGCTGTTAACGGTCCGGTTTGATAACTGCGAtt                                                        >  2:127569/1‑151 (MQ=255)                                                                                       aCATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGAtt                                                        <  2:571350/151‑1 (MQ=255)                                                                                           cACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATTATCTCCGGGGGCAAAAAACACGGACGATAGAAACTGTTTACGATCCGGGTCGATAAATGCGAt                                                         >  1:403166/1‑146 (MQ=255)                                                                                               cTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAAACCCGGACGAAAAAAGCTGGTAAAGAACCCGTTCGACAACTGCGATTGATCCa                                                  >  1:671538/1‑149 (MQ=255)                                                                                                  aaaaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCg                                             <  2:88869/151‑1 (MQ=255)                                                                                                  aaaaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAACCCCGGGAGATAGAAGCCGGTAAAGAACCGGGTCGATAACTGCGATTGATCCatat                                               >  2:220067/1‑149 (MQ=255)                                                                                                    aaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGc                                           <  1:755592/151‑1 (MQ=255)                                                                                                    aaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGc                                           <  2:400663/151‑1 (MQ=255)                                                                                                    aaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGGGGGAAAAAACACCGGCGATAGAAGCTGTTTAAGATCCGGTTCGGTAACTGCGCTTGGTTCATATCgg                                            >  1:212497/1‑150 (MQ=255)                                                                                                        aGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATg                                       <  2:212529/151‑1 (MQ=255)                                                                                                               atatTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTAAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCg                                <  1:650274/151‑1 (MQ=255)                                                                                                                       cgcgAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAAACACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAAATGCGATTGATCCATATCGGCAATGGCGGACGGCGCgggg                        >  2:438504/1‑151 (MQ=255)                                                                                                                                ataGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGAt               <  2:611425/151‑1 (MQ=255)                                                                                                                                ataGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAAACACGGACGATAGAAGCTGTTAACGATCCGGGTCGGTAACTGCGGTTGGTCCCTATCGGCAATGGGGATCGGCGCGGGGGTCAACGa                >  2:207124/1‑150 (MQ=255)                                                                                                                                ataGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGGGGCAAAAAACACGGGCGGTAGAAGCTGTTAACCATCCGGTTCGGTAACTGCGGTTGATCCATATCGGCAATGGCGATCGGGGCGAGGTTCa                    >  2:424281/1‑146 (MQ=255)                                                                                                                                  aGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATcc             <  2:713554/151‑1 (MQ=255)                                                                                                                                   gCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCt            <  2:386825/151‑1 (MQ=255)                                                                                                                                        cGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGt       <  2:500416/151‑1 (MQ=255)                                                                                                                                         ggCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGTa      <  2:640189/151‑1 (MQ=255)                                                                                                                                            gTCCAGCCCGGCATCCATCTCATCGGCGGTGGTCATATCCCGGGGCAAAAAACACGGACGGTAGAAGCTGTTTACGATCCGGTTCGATAACTGCGGATTATCCCTATCGGCAATGGGGGTCGGCGCGAGGGTCAAAGATCCTGGCGTaa     >  1:533436/1‑149 (MQ=255)                                                                                                                                             tCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGTGGCAAAAACCACCGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCCTATCGGCAATGGCGATCGGCGCGAGGGTCAACGATCCTGGCGTAAAgg  >  1:265410/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                  TCTGGATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGTAACGG  >  NC_000913/3626392‑3626682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑