Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,614	A→G	N248S (AAC→AGC)	yaiW →	microcin Bac7 uptake protein; outer membrane surface‑exposed lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,614	0	A	G	100.0%	152.6 / NA	43	N248S (AAC→AGC)	yaiW	microcin Bac7 uptake protein; outer membrane surface‑exposed lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

GTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGA  >  NC_000913/398466‑398746                                                                                                                                                     |                                                                                                                                     gTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGcg                                                                                                                                    >  1:238826/1‑151 (MQ=255)  tCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAgcgc                                                                                                                                   >  2:392332/1‑151 (MQ=255)       cAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTc                                                                                                                               <  1:423905/150‑1 (MQ=255)          gAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTa                                                                                                                           <  2:743380/151‑1 (MQ=255)             gTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAgg                                                                                                                        >  1:607162/1‑151 (MQ=255)             gTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAgg                                                                                                                        <  2:381022/151‑1 (MQ=255)                  cAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgc                                                                                                                   >  2:77038/1‑151 (MQ=255)                   aGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgcg                                                                                                                  >  2:339282/1‑151 (MQ=255)                      cgccgcGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgcggc                                                                                                                <  2:318384/150‑1 (MQ=255)                       gccgcGGCGGGTTGTGGGTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgcgg                                                                                                                 >  1:72840/1‑148 (MQ=255)                                ggTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGc                                                                                                     <  1:19287/151‑1 (MQ=255)                                    gtgGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACt                                                                                                  <  2:536273/150‑1 (MQ=255)                                       gTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGa                                                                                               >  1:357706/1‑150 (MQ=255)                                           ggTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTg                                                                                          >  2:125584/1‑151 (MQ=255)                                               cTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGAttt                                                                                      >  2:623617/1‑151 (MQ=255)                                                ttACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGAttt                                                                                      >  2:451368/1‑150 (MQ=255)                                                ttACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGAttt                                                                                      >  1:119778/1‑150 (MQ=255)                                                    cATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTc                                                                                 <  1:264511/151‑1 (MQ=255)                                                      tttACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAAtt                                                                                  <  2:81274/148‑1 (MQ=255)                                                             aaTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACg                                                                           <  1:523385/148‑1 (MQ=255)                                                             aaTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGAc                                                                         >  1:216856/1‑150 (MQ=255)                                                              aTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGAc                                                                         >  2:128402/1‑149 (MQ=255)                                                                 aTCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTa                                                                     >  1:659162/1‑150 (MQ=255)                                                                   cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTaa                                                                    <  2:18442/149‑1 (MQ=255)                                                                     cGCCAGTTATATCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGaa                                                                <  2:47766/151‑1 (MQ=255)                                                                             ataGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGaa                                                        <  2:153626/151‑1 (MQ=255)                                                                             ataGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGaa                                                        <  1:682479/151‑1 (MQ=255)                                                                              taGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGaa                                                        >  1:427402/1‑150 (MQ=255)                                                                               aGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGaaa                                                       <  1:508066/150‑1 (MQ=255)                                                                                   cACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGa                                                  <  2:347259/151‑1 (MQ=255)                                                                                      cGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACt                                               >  2:72591/1‑151 (MQ=255)                                                                                      cGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACt                                               <  1:430731/151‑1 (MQ=255)                                                                                      cGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACt                                               <  2:734253/151‑1 (MQ=255)                                                                                       gATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTg                                              >  1:566342/1‑151 (MQ=255)                                                                                        atatACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                             >  2:113811/1‑151 (MQ=255)                                                                                           taCCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCa                                           >  1:738731/1‑150 (MQ=255)                                                                                                      tGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGaa                                                                >  2:160658/1‑118 (MQ=255)                                                                                                      tGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGaa                                                                <  1:160625/118‑1 (MQ=255)                                                                                                        aTTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGc                             >  1:312530/1‑151 (MQ=255)                                                                                                                   ggctggTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgg                  >  1:579982/1‑151 (MQ=255)                                                                                                                     ctggTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgggg                >  2:122882/1‑151 (MQ=255)                                                                                                                            gCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCg         <  1:249665/151‑1 (MQ=255)                                                                                                                                 ccGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGc    <  1:14137/151‑1 (MQ=255)                                                                                                                                   gTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGa  <  2:339790/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                     GTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGA  >  NC_000913/398466‑398746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑