Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,913,871	T→C	T444A (ACT→GCT)	glmU ←	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,913,871	0	T	C	100.0%	146.9 / NA	41	T444A (ACT→GCT)	glmU	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/26);  total (15/26)

GGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGT  >  NC_000913/3913723‑3914018                                                                                                                                                     |                                                                                                                                                    gggCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGCGATTATGTTATCTACTCATCCCATGTGACCGGGTTAGCCGGCCAGCATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCt                                                                                                                                                   >  1:457824/1‑151 (MQ=255)  ggCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTg                                                                                                                                                  <  2:759826/151‑1 (MQ=255)    cgcCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCg                                                                                                                                                <  2:723029/151‑1 (MQ=255)          gAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCacac                                                                                                                                          <  2:144142/151‑1 (MQ=255)                tGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTg                                                                                                                                     <  2:287312/150‑1 (MQ=255)                tGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTg                                                                                                                                     <  1:124145/150‑1 (MQ=255)                 gTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGAt                                                                                                                                   >  1:353772/1‑151 (MQ=255)                       agTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTaa                                                                                                                             <  2:535002/151‑1 (MQ=255)                              ttATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCAttt                                                                                                                      >  1:636009/1‑151 (MQ=255)                                aTTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCAtttt                                                                                                                     <  2:746752/150‑1 (MQ=255)                                aTTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTc                                                                                                                    >  2:678794/1‑151 (MQ=255)                                aTTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTc                                                                                                                    <  1:621913/151‑1 (MQ=255)                                        aGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACa                                                                                                             <  1:446513/150‑1 (MQ=255)                                        aGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAt                                                                                                            <  2:78860/151‑1 (MQ=255)                                        aGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAACGCATTTTCGCCGACAt                                                                                                            <  1:677833/151‑1 (MQ=255)                                         gTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAtt                                                                                                           <  1:500930/151‑1 (MQ=255)                                         gTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAtt                                                                                                           <  2:434359/151‑1 (MQ=255)                                          ttACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAtt                                                                                                           >  2:186898/1‑150 (MQ=255)                                           tACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTa                                                                                                          <  2:262896/150‑1 (MQ=255)                                             cTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTAcg                                                                                                        <  1:759471/150‑1 (MQ=255)                                               gCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGTACACGGCTGATAGCTAATGCATTTTCGCCGACATTAcgc                                                                                                       <  2:772509/149‑1 (MQ=255)                                                cTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTc                                                                                                    >  2:575744/1‑151 (MQ=255)                                                                tATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCCGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCg                                                                                       >  1:561098/1‑148 (MQ=255)                                                                  tGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCgcagc                                                                                   >  1:742104/1‑150 (MQ=255)                                                                         tcctcATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTcg                                                                           >  1:540567/1‑151 (MQ=255)                                                                             cATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                                                       >  1:731874/1‑151 (MQ=255)                                                                                 ccATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTg                                                                   <  2:396922/151‑1 (MQ=255)                                                                                      tgACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTAc                                                              >  2:587061/1‑151 (MQ=255)                                                                                             gTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTAcc                                                       <  2:206763/151‑1 (MQ=255)                                                                                                   cGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGcc                                                 >  2:52983/1‑151 (MQ=255)                                                                                                          aaTCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCt                                          <  1:333668/151‑1 (MQ=255)                                                                                                          aaTCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCt                                          <  1:302833/151‑1 (MQ=255)                                                                                                            tCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTg                                         >  2:550219/1‑150 (MQ=255)                                                                                                             cACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGa                                        <  1:278094/150‑1 (MQ=255)                                                                                                                 ttttCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTc                                   >  1:759520/1‑151 (MQ=255)                                                                                                                                 acgCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAAcaca                   <  2:561618/151‑1 (MQ=255)                                                                                                                                   gCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtc                 >  1:23604/1‑151 (MQ=255)                                                                                                                                     cAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtcgt               <  2:636066/151‑1 (MQ=255)                                                                                                                                       gCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtcgtcg             <  2:687722/151‑1 (MQ=255)                                                                                                                                        ccTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGc            <  2:42386/151‑1 (MQ=255)                                                                                                                                                  gAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGt  <  2:291071/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGT  >  NC_000913/3913723‑3914018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑