Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,930,513	T→C	Q194R (CAG→CGG)	ravA ←	hexameric AAA+ MoxR family ATPase, putative molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,930,513	0	T	C	100.0%	151.5 / NA	42	Q194R (CAG→CGG)	ravA	hexameric AAA+ MoxR family ATPase, putative molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/20);  total (22/20)

GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCGGG  >  NC_000913/3930366‑3930659                                                                                                                                                    |                                                                                                                                                   ggTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGtt                                                                                                                                                 >  2:102697/1‑151 (MQ=255)    aaTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGAc                                                                                                                                              >  2:98650/1‑151 (MQ=255)        tATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTgg                                                                                                                                           <  2:585830/150‑1 (MQ=255)         aTCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACAAGATCGGGCAGCGTAATTTCCCCAATCGCATTCGGCCGGCGTTCATATTCTTCATCTGTGCCCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGt                                                                                                                                          <  1:121925/150‑1 (MQ=255)             aGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCa                                                                                                                                     <  2:659412/151‑1 (MQ=255)                 gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATgg                                                                                                                                  >  1:27672/1‑150 (MQ=255)                 gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                                 <  2:701680/151‑1 (MQ=255)                    ggCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAg                                                                                                                                <  2:145735/149‑1 (MQ=255)                           caTAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTc                                                                                                                       >  1:483442/1‑151 (MQ=255)                            aTAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCg                                                                                                                      >  2:615068/1‑151 (MQ=255)                              aaaaaTCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCt                                                                                                                    <  1:92809/151‑1 (MQ=255)                                aaaTCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCttt                                                                                                                  <  2:22845/151‑1 (MQ=255)                                   tCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATc                                                                                                               >  2:680757/1‑151 (MQ=255)                                           aaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACtt                                                                                                       >  2:461769/1‑151 (MQ=255)                                              aCATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTa                                                                                                     <  1:546427/150‑1 (MQ=255)                                                  gATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTaa                                                                                                <  1:372050/151‑1 (MQ=255)                                                  gATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTa                                                                                                 <  1:44051/150‑1 (MQ=255)                                                    tCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAcc                                                                                              >  1:292160/1‑151 (MQ=255)                                                        gCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACcac                                                                                            >  2:13676/1‑149 (MQ=255)                                                          aGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGAc                                                                                        <  2:26838/151‑1 (MQ=255)                                                               aaTTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAAt                                                                                    <  2:735575/150‑1 (MQ=255)                                                               aaTTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATc                                                                                   >  1:29657/1‑151 (MQ=255)                                                                    cACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCAt                                                                              <  1:199703/151‑1 (MQ=255)                                                                                  tGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACg                                                                >  1:538951/1‑151 (MQ=255)                                                                                   gCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATaa                                                                  <  2:417190/148‑1 (MQ=255)                                                                                    ccAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCt                                                              <  1:484706/151‑1 (MQ=255)                                                                                     cAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTa                                                                                                 >  2:236266/1‑115 (MQ=255)                                                                                     cAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTa                                                                                                 <  1:236233/115‑1 (MQ=255)                                                                                     cAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCtt                                                             >  2:675452/1‑151 (MQ=255)                                                                                             atatTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGActg                                                     >  1:412425/1‑151 (MQ=255)                                                                                                     cATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTg                                              <  1:173623/150‑1 (MQ=255)                                                                                                       tCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCtt                                           >  2:643075/1‑151 (MQ=255)                                                                                                          gtgACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCg                                        <  2:75150/151‑1 (MQ=255)                                                                                                            gACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGc                                      >  1:14344/1‑151 (MQ=255)                                                                                                            gACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGc                                      <  2:137497/151‑1 (MQ=255)                                                                                                             aCCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCa                                     <  1:39746/151‑1 (MQ=255)                                                                                                                 gCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTc                                 >  2:522001/1‑151 (MQ=255)                                                                                                                  cAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCg                                >  1:222168/1‑151 (MQ=255)                                                                                                                   aaGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGt                               >  2:504395/1‑151 (MQ=255)                                                                                                                           aGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgcc                       >  1:551516/1‑151 (MQ=255)                                                                                                                                             ttCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATc     >  2:169686/1‑151 (MQ=255)                                                                                                                                                aTCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCTACCAGCAGACGCATCggg  >  1:407217/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCGGG  >  NC_000913/3930366‑3930659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑