Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,246	T→C	V277A (GTT→GCT)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,246	0	T	C	100.0%	170.5 / NA	46	V277A (GTT→GCT)	rho	transcription termination factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

ACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGT  >  NC_000913/3967107‑3967388                                                                                                                                            |                                                                                                                                               aCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCg                                                                                                                                      >  1:92519/1‑150 (MQ=255)   cTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTc                                                                                                                                    >  1:182095/1‑150 (MQ=255)      tGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGt                                                                                                                                >  1:39854/1‑151 (MQ=255)       gACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGt                                                                                                                                >  1:211358/1‑150 (MQ=255)            aCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAgtg                                                                                                                          <  1:369400/151‑1 (MQ=255)            aCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCACTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAgtg                                                                                                                          >  2:668167/1‑151 (MQ=255)              ccGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGtt                                                                                                                        <  2:150128/151‑1 (MQ=255)                     tcGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggt                                                                                                                 >  1:211901/1‑151 (MQ=255)                      cGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGAcc                                                                                                                    <  1:775358/147‑1 (MQ=255)                      cGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggtg                                                                                                                >  2:593925/1‑151 (MQ=255)                        ccACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggtggt                                                                                                              <  2:595951/151‑1 (MQ=255)                               cAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAt                                                                                                        >  2:165286/1‑150 (MQ=255)                                aGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGc                                                                                                      <  2:601546/151‑1 (MQ=255)                                  gTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCa                                                                                                    <  1:69816/151‑1 (MQ=255)                                  gTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGCGGTGTGGATGcc                                                                                                     >  1:696556/1‑150 (MQ=255)                                           aTGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGc                                                                                           >  2:581088/1‑151 (MQ=255)                                             ggTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCAt                                                                                         <  2:445473/151‑1 (MQ=255)                                              gTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATc                                                                                        <  2:518132/151‑1 (MQ=255)                                                gATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCg                                                                                       <  1:252903/150‑1 (MQ=255)                                                 aTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTc                                                                                     <  2:745993/151‑1 (MQ=255)                                                  tCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTcc                                                                                    >  2:556329/1‑151 (MQ=255)                                                                cGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTg                                                                      >  2:49291/1‑151 (MQ=255)                                                                cGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTg                                                                      >  1:94980/1‑151 (MQ=255)                                                                         gAGCGCAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGTCCGGTGGTGTGGATGCCTACGCCCTGAATCGTCCGAAACGCTTCTTTAGTgcggcg                                                              <  1:330108/150‑1 (MQ=255)                                                                          aGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGcgcg                                                            <  2:506933/151‑1 (MQ=255)                                                                           gCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGt                                                           <  2:480755/151‑1 (MQ=255)                                                                            cacaAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTa                                                          <  2:422191/151‑1 (MQ=255)                                                                              caAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAAc                                                        >  1:45406/1‑151 (MQ=255)                                                                                agaaagaCGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGt                                                      <  1:713690/151‑1 (MQ=255)                                                                                          tATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAggg                                             <  1:548842/150‑1 (MQ=255)                                                                                           aTCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGggcg                                           <  1:183622/151‑1 (MQ=255)                                                                                               ttCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGggcgg                                          >  1:392874/1‑148 (MQ=255)                                                                                                    cTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTg                                   >  2:597538/1‑150 (MQ=255)                                                                                                      cGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGAcc                                >  1:24864/1‑151 (MQ=255)                                                                                                          tCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCAtt                             >  1:671505/1‑150 (MQ=255)                                                                                                           ccATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTa                            <  2:329258/150‑1 (MQ=255)                                                                                                           ccATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTAt                           <  1:540491/151‑1 (MQ=255)                                                                                                                   cGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACg                    <  2:84388/150‑1 (MQ=255)                                                                                                                       tGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGgcgc                >  1:746353/1‑150 (MQ=255)                                                                                                                          cgcgcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTAt            <  2:17461/151‑1 (MQ=255)                                                                                                                           gcgcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATc           <  2:37763/151‑1 (MQ=255)                                                                                                                             gcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGa         <  1:668109/151‑1 (MQ=255)                                                                                                                               gcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCGGCCTGACCATTATCGCGACGGCGCTTATCGATa       <  1:91651/151‑1 (MQ=255)                                                                                                                               gcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATa       <  2:397597/151‑1 (MQ=255)                                                                                                                                 ttACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATAcc     >  1:147350/1‑151 (MQ=255)                                                                                                                                    caacaCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGt  >  2:403709/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                               ACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGT  >  NC_000913/3967107‑3967388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑