Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,998,071 G→T G30V (GGC→GTC)  uvrD → DNA‑dependent ATPase I and helicase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,0710GT100.0% 155.0 / NA 43G30V (GGC→GTC) uvrDDNA‑dependent ATPase I and helicase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/23);  total (20/23)

TGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAA  >  NC_000913/3997930‑3998210
                                                                                                                                             |                                                                                                                                           
tGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGgcggtcgcggg                                                                                                                                       >  1:574513/1‑148 (MQ=255)
tGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCa                                                                                                                                     <  2:76696/150‑1 (MQ=255)
 ggCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAg                                                                                                                                    <  1:635588/150‑1 (MQ=255)
           gTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGAcg                                                                                                                          >  2:336385/1‑150 (MQ=255)
             tGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGGGGTAAGAcgcg                                                                                                                        >  1:465765/1‑150 (MQ=255)
                   tctcCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACt                                                                                                                  >  1:479709/1‑150 (MQ=255)
                   tctcCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTg                                                                                                                 >  2:573625/1‑151 (MQ=255)
                   tctcCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCAGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACt                                                                                                                  <  1:71155/150‑1 (MQ=255)
                      ccGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTg                                                                                                              <  1:646366/151‑1 (MQ=255)
                       cGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGc                                                                                                             >  2:719853/1‑151 (MQ=255)
                        gCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGc                                                                                                             >  2:599573/1‑150 (MQ=255)
                           cAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCg                                                                                                         >  1:376651/1‑151 (MQ=255)
                           cAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCg                                                                                                         >  2:400687/1‑151 (MQ=255)
                               cTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATc                                                                                                     >  1:591997/1‑151 (MQ=255)
                                 aTTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCg                                                                                                    <  2:55711/150‑1 (MQ=255)
                                  tttttACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGcc                                                                                                  >  1:150162/1‑151 (MQ=255)
                                       aCGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGtt                                                                                             >  2:476954/1‑151 (MQ=255)
                                             cggTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAg                                                                                       <  2:451556/151‑1 (MQ=255)
                                                 gCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGt                                                                                    <  1:624039/150‑1 (MQ=255)
                                                     aTGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGaaa                                                                               <  1:294394/151‑1 (MQ=255)
                                                     aTGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGaa                                                                                >  1:767040/1‑150 (MQ=255)
                                                             ttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTc                                                                        >  1:620674/1‑150 (MQ=255)
                                                                   ccTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATAc                                                                 <  2:600777/151‑1 (MQ=255)
                                                                     tGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTc                                                               >  2:257921/1‑151 (MQ=255)
                                                                            cAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATg                                                        >  1:573302/1‑151 (MQ=255)
                                                                                  tAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGt                                                   <  1:188476/150‑1 (MQ=255)
                                                                                   aaTGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGa                                                 <  2:499431/151‑1 (MQ=255)
                                                                                       aCAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGtt                                             <  2:62952/151‑1 (MQ=255)
                                                                                       aCAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGGGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCggggggg                                               >  1:751085/1‑144 (MQ=255)
                                                                                        cAATCAGCGCGCAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGGGGGAAGACGCGCGTACTGGTGAATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATTCTCGATTATGGCg                                                     >  1:601611/1‑142 (MQ=255)
                                                                                         aaaCAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTa                                           <  1:81122/151‑1 (MQ=255)
                                                                                          aaCAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGGAAACTGCTCGCCATACTCGATTATGGCgggcgggctt                                            >  1:40174/1‑141 (MQ=255)
                                                                                                gcgAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACcaacaa                                    <  2:748966/151‑1 (MQ=255)
                                                                                                gcgAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACcaacaa                                    <  2:364642/151‑1 (MQ=255)
                                                                                                gcgAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGGAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCggggggggt                                             >  1:619197/1‑135 (MQ=255)
                                                                                                  gAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGGGCGGTTTCcaaca                                     >  1:401937/1‑148 (MQ=255)
                                                                                                    aGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcg                                <  2:214444/151‑1 (MQ=255)
                                                                                                      cGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggc                              <  1:561160/151‑1 (MQ=255)
                                                                                                      cGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggc                              <  2:74337/151‑1 (MQ=255)
                                                                                                       ggTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggcg                             <  1:156107/151‑1 (MQ=255)
                                                                                                          ggCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggcggcg                          <  2:574568/151‑1 (MQ=255)
                                                                                                                       agcaACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATc             <  1:476903/151‑1 (MQ=255)
                                                                                                                        gcaACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCg            <  2:381568/151‑1 (MQ=255)
                                                                                                                                  ggTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCaa  <  2:685021/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAA  >  NC_000913/3997930‑3998210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑