Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	440,471	A→G	Y211H (TAT→CAT)	ispA ←	geranyltranstransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	440,471	0	A	G	100.0%	188.3 / NA	49	Y211H (TAT→CAT)	ispA	geranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/30);  total (19/30)

CTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGT  >  NC_000913/440327‑440614                                                                                                                                                 |                                                                                                                                                cTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTc                                                                                                                                           >  2:575013/1‑151 (MQ=255)    gCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCCCCCCATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGa                                                                                                                                         <  1:499699/150‑1 (MQ=255)    gCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAg                                                                                                                                        <  1:87707/151‑1 (MQ=255)       cAAGGCCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGt                                                                                                                                       <  2:592324/149‑1 (MQ=255)            cccAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCa                                                                                                                                <  2:474458/151‑1 (MQ=255)               aGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCag                                                                                                                               >  2:384587/1‑149 (MQ=255)                   gTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacg                                                                                                                         <  1:31496/151‑1 (MQ=255)                    tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacg                                                                                                                         <  2:36413/150‑1 (MQ=255)                    tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacg                                                                                                                         >  1:36382/1‑150 (MQ=255)                                aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                                                                                            >  2:38102/1‑151 (MQ=255)                                 cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                                                                                           >  1:247530/1‑151 (MQ=255)                                  ttttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTcc                                                                                                          >  1:708264/1‑151 (MQ=255)                                    ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCgg                                                                                                        >  1:216903/1‑151 (MQ=255)                                     tACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                                                                                       >  2:466059/1‑151 (MQ=255)                                     tACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                                                                                       >  2:181137/1‑151 (MQ=255)                                      aCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                                                                                       <  2:511806/150‑1 (MQ=255)                                         aaGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCtt                                                                                                   <  2:212338/151‑1 (MQ=255)                                          aGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCtt                                                                                                   >  1:418772/1‑150 (MQ=255)                                             tGCTGGTCGGCTCCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAt                                                                                                <  2:443747/150‑1 (MQ=255)                                             tGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAt                                                                                                <  1:413647/150‑1 (MQ=255)                                                     ggCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAgg                                                                                       <  2:431787/151‑1 (MQ=255)                                                       cACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGc                                                                                      <  2:629038/150‑1 (MQ=255)                                                              gCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcg                                                                              <  1:2597/151‑1 (MQ=255)                                                                gTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACggcggc                                                                            <  2:144788/151‑1 (MQ=255)                                                                gTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACggcggc                                                                            <  2:323532/151‑1 (MQ=255)                                                                   ttCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGcgcg                                                                         >  2:633644/1‑151 (MQ=255)                                                                          cGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt                                                                  <  2:180262/151‑1 (MQ=255)                                                                              gCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcg                                                               <  2:373144/150‑1 (MQ=255)                                                                                 gTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCgg                                                           <  2:238584/151‑1 (MQ=255)                                                                                   aTCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGt                                                          <  2:446185/150‑1 (MQ=255)                                                                                    tctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGttt                                                        >  1:575355/1‑151 (MQ=255)                                                                                       cccACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTa                                                      <  1:39286/150‑1 (MQ=255)                                                                                       cccACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTAt                                                     >  1:411263/1‑151 (MQ=255)                                                                                           ccacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACg                                                 <  1:101793/151‑1 (MQ=255)                                                                                              caTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATg                                              >  1:76725/1‑151 (MQ=255)                                                                                                  cAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAAt                                           >  1:506491/1‑150 (MQ=255)                                                                                                   aGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATAc                                         >  2:114308/1‑151 (MQ=255)                                                                                                      aTGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCt                                      <  2:386447/151‑1 (MQ=255)                                                                                                        gTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCa                                    <  2:247811/151‑1 (MQ=255)                                                                                                               tGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGt                             >  2:85821/1‑151 (MQ=255)                                                                                                                   ccTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCag                         <  2:432027/151‑1 (MQ=255)                                                                                                                      ggAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCaga                        <  2:680075/149‑1 (MQ=255)                                                                                                                           gcaaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACgt                 <  1:302557/151‑1 (MQ=255)                                                                                                                            caaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACgtg                >  1:520510/1‑151 (MQ=255)                                                                                                                              aggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGtt              >  2:601885/1‑151 (MQ=255)                                                                                                                              aggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGtt              <  2:633528/151‑1 (MQ=255)                                                                                                                               ggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGttt             <  2:338453/151‑1 (MQ=255)                                                                                                                                    aTGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCtt        <  1:333644/151‑1 (MQ=255)                                                                                                                                             gCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGt  <  2:740580/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                CTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGT  >  NC_000913/440327‑440614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑