Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,009,947	A→G	T260A (ACG→GCG)	pldB →	lysophospholipase L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,009,947	0	A	G	100.0%	138.4 / NA	40	T260A (ACG→GCG)	pldB	lysophospholipase L2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/19);  total (21/19)

CCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTT  >  NC_000913/4009805‑4010094                                                                                                                                               |                                                                                                                                                    ccACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACg                                                                                                                                             >  1:508578/1‑151 (MQ=255)            aGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGc                                                                                                                                   >  1:541682/1‑150 (MQ=255)                atatCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCt                                                                                                                              <  1:192055/151‑1 (MQ=255)                  atCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTg                                                                                                                             <  1:699463/150‑1 (MQ=255)                    cGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGa                                                                                                                            >  1:643814/1‑149 (MQ=255)                     gACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAaga                                                                                                                         <  1:690245/151‑1 (MQ=255)                      aCGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAag                                                                                                                          >  2:760689/1‑149 (MQ=255)                       cGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAgg                                                                                                                       >  1:48508/1‑151 (MQ=255)                             ttACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                                                                 >  1:376073/1‑151 (MQ=255)                              tACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgt                                                                                                                >  2:492716/1‑151 (MQ=255)                                 gCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtggt                                                                                                             <  1:724434/151‑1 (MQ=255)                                   ttCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGtggtgg                                                                                                           >  2:485791/1‑151 (MQ=255)                                       aTGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGa                                                                                                          <  2:693202/148‑1 (MQ=255)                                               gaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCgcatgc                                                                                               <  1:29869/151‑1 (MQ=255)                                                aCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCgcatgc                                                                                               >  2:340946/1‑150 (MQ=255)                                                              gTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTtgtg                                                                                <  2:376120/151‑1 (MQ=255)                                                               tCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTtgtg                                                                                >  1:290031/1‑150 (MQ=255)                                                                    gggCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTcc                                                                          <  2:216664/151‑1 (MQ=255)                                                                     ggCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCg                                                                         <  2:86088/151‑1 (MQ=255)                                                                             tACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCAccgccg                                                                 >  1:42439/1‑151 (MQ=255)                                                                                 aTTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCggg                                                             >  1:129052/1‑151 (MQ=255)                                                                                    gggTACGCGAAAGTATTCTGGCTTGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCa                                                          <  2:689599/151‑1 (MQ=255)                                                                                           cgAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTc                                                   >  2:538368/1‑151 (MQ=255)                                                                                            gAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCg                                                  >  1:311002/1‑151 (MQ=255)                                                                                             aaaGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCg                                                  >  2:237656/1‑150 (MQ=255)                                                                                                   ttCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCTGCGGGCCATCCTGTCGAaggagg                                           >  2:436460/1‑151 (MQ=255)                                                                                                       ggctggcGAACAGGTGTTAGCCGGTGAGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACgg                                       <  2:208311/151‑1 (MQ=255)                                                                                                              gAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTgg                                <  1:13900/151‑1 (MQ=255)                                                                                                              gAACAGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTgg                                <  2:537236/151‑1 (MQ=255)                                                                                                                  aGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAAt                            >  2:209055/1‑151 (MQ=255)                                                                                                                  aGGTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAAt                            <  2:331713/151‑1 (MQ=255)                                                                                                                   ggTGTTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAAtt                           <  1:340912/151‑1 (MQ=255)                                                                                                                     tgtTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCGCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTaa                             <  1:439224/147‑1 (MQ=255)                                                                                                                      gtTAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTaa                         <  2:758603/150‑1 (MQ=255)                                                                                                                        tAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAg                       >  2:770809/1‑150 (MQ=255)                                                                                                                        tAGCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAg                       >  1:652267/1‑150 (MQ=255)                                                                                                                          gCCGGTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTg                    <  1:524806/151‑1 (MQ=255)                                                                                                                             ggTGCGGGTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCtt                 <  1:706685/151‑1 (MQ=255)                                                                                                                                  gggTGATGACGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCAt            >  1:641925/1‑151 (MQ=255)                                                                                                                                     tgatgaCGCCGCGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATg           >  1:133628/1‑149 (MQ=255)                                                                                                                                           cgccgcgccaACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCtt   <  1:484406/151‑1 (MQ=255)                                                                                                                                            gccgcgccaACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCttt  <  2:719723/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    CCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTT  >  NC_000913/4009805‑4010094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑